Download Surface Plasmon Resonance Measurement of Neutravidin Layer on

7th Symposium of European Vacuum Coaters -- Anzio (IT), Oct. 2nd – Oct. 4th , 2006
Surface Plasmon Resonance Measurement
of Neutravidin Layer on Gold Thin Film
A. Taormina, A. Busacca, S. Riva Sanseverino
Dipartimento Ingegneria Elettrica, Elettronica e delle TLC, Università di Palermo
Viale delle Scienze - 90128 Palermo
A.C. Cino, G. D’Arpa, S. Guarino, S. Maddiona, A. Parisi, A. Piazza, S. Sclafani
CRES - Centro per la Ricerca Elettronica in Sicilia
Via Regione Siciliana 49 - 90046 Monreale (PA)
The surface plasmon resonance (SPR) technique is an optical method that can be used to
measure the refractive index of organic nano-layers adsorbed on a thin metal film. Although
there are many techniques for measuring biomolecular interactions, SPR-based techniques
play a central role in many current biosensing experiments, since they are most suited to
sensitive and quantitative kinetic measurements.
Here we give some results from the analysis and numerical elaboration of SPR data
regarding the deposition of Neutravidin protein on the gold thin film of a Spreeta™ cell, a
SPR-based sensor head developed by Texas Instruments and distributed for research purposes.
The deposition of Neutravidin on the gold thin film is required in order to fix a desired
biotinylated Antibody, which in turn can react selectively with its specific Antigen. Actually,
the Neutravidin is adsorbed on the sensing surface and establishes a strong tie with the Biotin,
which is conjugated with the Antibody.
After a brief discussion of the principles of SPR and a description of the system and fluid
circulation setup, we show results of real time interaction between gold thin film and
Neutravidin, pointing out that a proper choice of the protein-film layer thickness on the gold
surface is critical in order to obtain a detectable signal from the following Antigen-Antibody
reaction.