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New CFSE-based assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell population by Inge Jedema, Nicole M. van der Werff, Renée M. Y. Barge, Roel Willemze, and J. H. Frederik Falkenburg Blood Volume 103(7):2677-2682 April 1, 2004 ©2004 by American Society of Hematology Correlation between the CFSE-based cytotoxicity assay and the conventional 51Cr release assay. Inge Jedema et al. Blood 2004;103:2677-2682 ©2004 by American Society of Hematology Manipulation of the proliferative status of primary leukemic cells by cytokines. Inge Jedema et al. Blood 2004;103:2677-2682 ©2004 by American Society of Hematology Analysis of the specific cytotoxicity induced by a T-cell clone in different cell types within a heterogeneous target cell population. Inge Jedema et al. Blood 2004;103:2677-2682 ©2004 by American Society of Hematology Analysis of the HLA restriction of CTL-induced target cell killing using the CFSE-based cytotoxicity assay. Inge Jedema et al. Blood 2004;103:2677-2682 ©2004 by American Society of Hematology Susceptibility to CTL-induced lysis of chronic-phase CML. A conventional 4-hour 51Cr release assay was performed by using the MNCs derived from the peripheral blood of a male patient with chronic-phase CML positive for HLA-A1 as target cell population. Inge Jedema et al. Blood 2004;103:2677-2682 ©2004 by American Society of Hematology Differential recognition of proliferating CD34+ CML precursors. Inge Jedema et al. Blood 2004;103:2677-2682 ©2004 by American Society of Hematology