Download work plan and methodology

3. Aim and Objectives:
1.
The specific objective of the work will optimization of process parameter viz. concentration of
monomer and various supporting electrolytes (dopant) like potassium nitrate, sodium nitrate
and nitric acid etc.
2.
Optimize the pH of electrolyte; current density etc for the synthesis of conducting polymer
base thin film uses it as a biosensor.
3.
Synthesis of conducting polymer film by using Polypyrrole, Polythiophene and their copolymer .
4.
Development of various enzymes sensor.
4. WORK PLAN AND METHODOLOGY
In existing experimental setup we synthesized conducting polymer matrix with various
dopants and composite dopants can be used for the development if the various biosensor for
detection of Glucose, Cholesterol, Lactate, Urea, Hemoglobin etc. Our efforts are also directed
to develop mobile equipment for the uniquely design biosensor.
have been recently proposed for
The conducting polymers
biosensing applications because of a number of favorable
characteristics, such as direct and easy deposition on sensor electrode by electrochemical
oxidation of monomer, Control of thickness and Redox conductivity. Aqueous electrochemical
process is an environment friendly and efficient technique being used for synthesis of
conducting polymer. Conducting polymer film is incorporated in biosensor to facilitate electron
transfer from biocomponent to the electrode surface for the incorporation of mediator and in
the
immobilization of enzyme. Conducting Polymer Films are capable of conducting electronic
charge such as
1. Aliphatic polymer e.g. Polyacetylene
2. Aromatic polymer e.g. Polyaniline and
3. Heterocyclic polymer e.g. Polypyrrole
Polymer
films
will
prepared
by
galvanostatic
electrochemical
synthesis,
which
provides a constant oxidative current at the anode. The electrochemical deposition of monomer
and their copolymer films will carried out by using a galvanostatic technique at temperature 27
°C in a one-compartment, three-electrode glass cell. The ITO coated glass plate will have to use
as a working electrode, platinum foil as a counter electrode and Ag/AgCl as a reference
electrode. The electrolyte solution will prepare in deionized water with optimum parameter.
After synthesis the polymer coated electrodes will rinsed thoroughly in deionized water dried in
cold air and then used for subsequent characterization. The synthesized composite films will
subjected to various characterization viz. galvanostatic electrochemical technique, electrical
conductivity. The FTIR,SEM,X-RD etc methods use for characterization.