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Questions for candidates applying for MSF laboratory technicians’ positions Name: _ _ _ _ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ _ Date: _ _ _ _ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ _ Please be as specific / detailed as possible when answering the questions below. Thank you in advance for this additional information. Here is a list with essential skills that are often needed in MSF context, to a higher or less extent depending on the focus of the mission. Please describe your experiences in these techniques in detail. 1. Management Including (non-exhaustive list): -Training -HR management (evaluations, managing difficult relationships, coaching, recruitment) -Supply management (ordering, consumption calculation, stock management) - Equipment maintenance and troubleshooting -… 2. Quality assurance / quality control QA/QM systems used QC methodologies, including Levey-Jennings/Westgard rules, external QC/proficiency testing, any other systems 3. Haematology Hb determination (colorimetric), WBC count (manual dilution and counting – counting chamber), manual blood differential, automated methods (please list instruments and tests) 4. Transfusion Donor recruitment, donor screening including infectious disease testing, Blood grouping, cross-match, blood-bank management 5. Tuberculosis – please also complete ‘Laboratory skills related to Tuberculosis diagnosis’ at the end of this form AFB examinations (smearing, Ziehl Neelsen and fluorescent staining and microscopy, culture and drug sensitivity testing (please list method incl LJ, MGIT or any other), GeneXpert, Hain test, any other PCR) 6. Malaria Microscopic smear examinations (thin film and thick film – Giemsa and or Field staining), species differentiation RDT; Other methods, incl e.g. PCR 7. Stool Parasitology List any experience, from rapid tests to microscopy and culture 8. Urine analysis List any experience, from rapid tests to microscopy and culture 9. Bacteriology : CSF, Pleural... fluid examinations Wet preparation: cell count Differential count Gram stain Culture and antibiograms/ drug sensitivity testing 10. HIV-1 / AIDS Diagnosis (rapid tests, ELISA, confirmation tests?) CD4 determinations Viral load DNA PCR Other methologies? 11. Outbreak investigation Sample packaging and transport, any other experiences Laboratory skills related to Tuberculosis diagnosis Please answer the following questions by selecting Yes or No. Feel free to comment / give additional information, 1. Specific questions related to TB microscopy and culture Comments Ziehl-Nielsen Microscopy Other techniques for Mycobacteria microscopy: - Bleach Concentration - Auramine fluorescence Yes / No Quality Control Yes / No Mycobacterium culture - Lowenstein-Jensen (LJ) - MGIT - Other (TLA, MODS...) - The “associated” techniques: Mcfarland densitometry, Blood Agar plates, etc Yes / No Yes / No Yes / No Yes / No Yes / No Yes / No Species confirmation: - Niacin, Utrease, Nitrate Reductace, etc - Chord formation (microscope) - Dipsticks (Capillia) - Other Yes / No Yes / No Yes / No Yes / No Yes / No Yes / No Drug Sensitivity Test: - MGIT SIRE - LJ proportion method - LJ concentration method - Agar - Other Yes / No Yes / No Yes / No Yes / No Yes / No Biosafety Basic Rules Lab setting Cabinets other Yes / No Yes / No Yes / No Yes / No Yes / No Management of STI’s using a syndromic approach Yes / No PMTCT if going to an HIV project Yes / No 2. Other lab tests Comments PCR based tests HAIN MTBDRplus Other Yes / No Yes / No Yes / No ELISA Yes / No Rapid Diagnostic Tests (RDT) Yes / No CD4 FACSCount FACSCAlibur EPICSXL Other Yes / No Yes / No Yes / No Yes / No Yes / No Biochemistry Spectrophotometer other Yes / No Yes / No Yes / No Any addition or comments welcome: