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Questions for candidates applying for MSF laboratory technicians’ positions
Name:
_ _ _ _ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ _
Date:
_ _ _ _ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ __ _ _
Please be as specific / detailed as possible when answering the questions below. Thank you in
advance for this additional information.
Here is a list with essential skills that are often needed in MSF context, to a higher or less
extent depending on the focus of the mission. Please describe your experiences in these
techniques in detail.
1. Management
Including (non-exhaustive list):
-Training
-HR management (evaluations, managing difficult relationships, coaching, recruitment)
-Supply management (ordering, consumption calculation, stock management)
- Equipment maintenance and troubleshooting
-…
2. Quality assurance / quality control
QA/QM systems used
QC methodologies, including Levey-Jennings/Westgard rules, external QC/proficiency testing, any
other systems
3. Haematology
Hb determination (colorimetric), WBC count (manual dilution and counting – counting chamber),
manual blood differential, automated methods (please list instruments and tests)
4. Transfusion
Donor recruitment, donor screening including infectious disease testing, Blood grouping, cross-match,
blood-bank management
5. Tuberculosis – please also complete ‘Laboratory skills related to Tuberculosis diagnosis’ at
the end of this form
AFB examinations (smearing, Ziehl Neelsen and fluorescent staining and microscopy, culture and
drug sensitivity testing (please list method incl LJ, MGIT or any other), GeneXpert, Hain test, any other
PCR)
6. Malaria
Microscopic smear examinations (thin film and thick film – Giemsa and or Field staining), species
differentiation
RDT; Other methods, incl e.g. PCR
7. Stool Parasitology
List any experience, from rapid tests to microscopy and culture
8. Urine analysis
List any experience, from rapid tests to microscopy and culture
9. Bacteriology : CSF, Pleural... fluid examinations
Wet preparation: cell count
Differential count
Gram stain
Culture and antibiograms/ drug sensitivity testing
10. HIV-1 / AIDS
Diagnosis (rapid tests, ELISA, confirmation tests?)
CD4 determinations
Viral load
DNA PCR
Other methologies?
11. Outbreak investigation
Sample packaging and transport, any other experiences
Laboratory skills related to Tuberculosis diagnosis
Please answer the following questions by selecting Yes or No. Feel free to comment / give additional
information,
1.
Specific questions related to TB microscopy and culture
Comments
Ziehl-Nielsen Microscopy
Other techniques for Mycobacteria
microscopy:
- Bleach Concentration
- Auramine fluorescence
Yes / No
Quality Control
Yes / No
Mycobacterium culture
- Lowenstein-Jensen (LJ)
- MGIT
- Other (TLA, MODS...)
- The “associated” techniques:
Mcfarland densitometry,
Blood Agar plates, etc
Yes / No
Yes / No
Yes / No
Yes / No
Yes / No
Yes / No
Species confirmation:
- Niacin, Utrease, Nitrate
Reductace, etc
- Chord formation (microscope)
- Dipsticks (Capillia)
- Other
Yes / No
Yes / No
Yes / No
Yes / No
Yes / No
Yes / No
Drug Sensitivity Test:
- MGIT SIRE
- LJ proportion method
- LJ concentration method
- Agar
- Other
Yes / No
Yes / No
Yes / No
Yes / No
Yes / No
Biosafety
Basic Rules
Lab setting
Cabinets
other
Yes / No
Yes / No
Yes / No
Yes / No
Yes / No
Management of STI’s using a
syndromic approach
Yes / No
PMTCT if going to an HIV project
Yes / No
2.
Other lab tests
Comments
PCR based tests
HAIN MTBDRplus
Other
Yes / No
Yes / No
Yes / No
ELISA
Yes / No
Rapid Diagnostic Tests (RDT)
Yes / No
CD4
FACSCount
FACSCAlibur
EPICSXL
Other
Yes / No
Yes / No
Yes / No
Yes / No
Yes / No
Biochemistry
Spectrophotometer
other
Yes / No
Yes / No
Yes / No
Any addition or comments welcome: