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CURRICULUM VITAE
Vladimir F. Eremin, Ph.D., M.D.
1. Personal information
Family name:
Eremin
First name:
Vladimir
Patronymic name: Fedorovich
Personal Data:
Home Address:
8 Suharevskja st., ap.107, Minsk,
220022, Belarus.
Business Address: Republican Research & Practical Centre for Epidemiology & Microbiology
(RRPCEM), 23 Filimonova st., Minsk, 220114, Belarus
Date of Birth:
May 20, 1958
Place of Birth:
Nerchinsk, Russian
Citizenship:
Byelorussian
Marital Status:
Married, two children (sons, 1982, 1990)
Phone ( in Minsk ):
Fax:
E.mail.:
(H) (+37517) 315-84-56
(W) (+37517) 268 04 16
(+37517) 268-04-16
[email protected]
2. Academic background
Education:
Diploma doctor of medicine in hygiene and epidemiology
Medical Institute in Minsk, 1976 – 1982;
Ph.D. Degree (candidate of biological science) for the thesis “Prepare antigen for
bovine leukemia virus diagnosis”, Moscow, May 26 1986;
Postgraduate special course at the D.I. Ivanovsky Institute of Virology,
Moscow 1982 – 1985;
Doctor of medical science for the thesis “HIV-infection in
Belarus (virological, serological and molecular-biological
investigations)”, Minsk April 15 1998;
Current Appointment: Head of laboratory: HIV and accompanying infection diagnosis
23 Filimonova st., Republican Research & Practical Centre for
Epidemiology & Microbiology, Minsk, 220114, Belarus.
Certification &
Licensure:
Diploma IV No. 175241
26 June, 1982, Minsk
Diploma of Ph.D. BL No. 017088
5 November, 1986, Moscow
Diploma of Doctor of Medical Science DN No. 000057
15 April 1998, Minsk
Certificate of Senior Researcher
SN 074937 17 April 1992, Moscow
Research work: In 1982 I began my scientific research work as a post - graduate student at the
Retroviruses laboratory at the Research Institute for Virology, named after D.I. Ivanovsky of the
Academy of Medical Sciences of the USSR in Moscow. My Investigations were mainly devoted
to bovine leukemia virus (BLV) infection diagnosis.
In 1985 I started working with HIV: cultivation, purification, preparing EIA kits at D.I.
Ivanovsky Institute of Virology. In May 26 th 1986 I defended my PhD thesis “Prepare antigen
for bovine leukemia virus diagnosis”, and got a scientific degree of candidate of biological
science (Ph D).
From 1985 to 1987 I worked at the Hepatitis A and Rotaviruses diagnosis laboratory at the
Byelorussian research Institute for Epidemiology and Microbiology. My investigations dealt
with diagnosis of Hepatitis A and rotaviruses infections.
In 1987 a new AIDS laboratory was organized at our Institute and I started working in it as a
senior scientific researcher.
At present - head of Laboratory for HIV and accompanying infection diagnosis, my
investigations are focused on wild HIV strains isolation and pheno- and genotyping from patients
screened on the territory of Belarus, as well as opportunistic infections (HSV, Varizella Zoster,
CMV, Chlamydia, Hepatitis B, C, D and so on) diagnosis. Nowadays, more than 15500 HIV
cases are reported in Belarus. We had only 113 HIV cases from 1986 to January 1996 and more
1900 cases from May 1996 to May 1997. IDU’s - the main reason of this outbreak.
I isolated more than 100 HIV-I and HIV-II strains. Two rapid/high HIV-I strains were the first
in the former USSR. Now they are used in Western blot kit preparation in Belarus.
To study HIV-neuroinfection we used primary human astrocyte cell cultures. We proved that
astrocytes can be infected in two ways: by means of receptors and phagocytosis of infected cells
and it makes it possible to get latent and productive forms of HIV neuroinfection, meanwhile
astrocytes can serve as HIV depot in CNS just like macrophages in blood. We revealed
multinuclear giant cells in vitro (in astrocytes), like those in brains of died patients.
At present, HIV pheno-, sero- and genotyping, search of anti-HIV compounds, HIV, hepatitis
B, C, HPV molecular epidemiology, as well as HIV, hepatitis C, B resistance – all these
problems lie in the sphere of my scientific interests.
As for my own experience and practice, I became proficient in the following methods:
oligonucleiteds synthesis on expedite nucleic acid synthesis system (PerSeptive Biosystems,
USA), HIV isolation, lymphoid and astrocytes cell cultures cultivation, SDS-PAGE, protein
chromatography purifying of proteins, HIV antigens and anti-HIV antibodies testing in cell
cultures and blood (EIA – Ag+Ag/Ab), RT-PCR, PCR, peptides EIA, MTT, XTT farmazan
assay for searching of anti-HIV compounds, sequencing ABI Prism 3100, 3500 (ViroSeq HIV-1
genotyping System v.2.0, Sequencing Analysis, BioEdit, SeqScape, Mega6- Software).
The Belorussian research and practical center for epidemiology and microbiology is
leading scientific institution in Republic of Belarus. The basic lines of activity of Center is
maintenance of epidemiological well-being in the country, control over the most significant
infectious diseases: HIV, hepatitis B, C, Influenza, rotaviruses, measles, Papilloma viruses,
Togaviruses, Arboviruses, Herpesviruses, Hantaviruses, and so on. The institute is engaged in
working out and manufacture of diagnostic preparations which are used both in Belarus, and in
Russia, other CIS countries.
Research practice:
11 - 12. 1987 - National Institute of Health, Tokyo, Japan (prof. Ketamura)
01 - 02. 1990 - National Institute of Health, Bethesda, Maryland ( Dr. David Asher )
03 - 04. 1991 - Institute of Physiology Chemistry, Mainz, Germany ( prof. W-E. G. Muller )
09 - 11. 1993 - AIDS Center, Robert Koch Institute, Berlin, Germany ( prof. G. Pauli )
Vladimir F. Eremin, Ph.D., M.D.
List of main publications
Patents:
1. Sharabrin O.I., Sukharev O.I., Gunenkov V.V., Osidze D.F., Sedov V.A., Kasjuk V.I., Sirota
V.F., Parphanovich M.I., Zhdanov V.M., Ratushkina L.S., Eremin V.F.(1986): Media for cell
culture and virus grow. USSR Patent, No.1228487 dated 3.01.1986.
2. Parphanovich M.I., Gunenkov V.V., Osidze D.F., Zhdanov V.M., Gushin V.N., Nochevni
V.T., Eremin V.F., Ratushkina L.S., Laktionov A.M. (1986): Method for obtaining of bovine
leukemia virus antigen. USSR Patent, No.1298983 dated 22.11.1986.
3. Shket V.A., Gudkov V.G., Rytik P.G., Eremin V.F. (1990): Method of Hepatitis A virus
isolation in cell culture. USSR Patent, No.1577519 dated 8.04.1990.
4. Rytik P.G., V.F. Eremin., Kolomiez N.D., Popov S.A. (1991): HIV strain for diagnostic kits
preparing. USSR Patent, No.1703686, dated 8.09.1991.
5. Remedy against HSV/ Patent ¹а 19990066, January 21, 2004 // Votjakov V.I., Losev Y.P.,
Phedulov A.S., Kvacheva Z.B., Nikolaeva S.N., Boreko E.I., Leontiev Y.M., Titov L.P., Eremin
V.F.
Articles:
1. Bukrinsky M.I., Zlobin A.Yu.,Arakelov S.A., Popov S.A., Chaplinskas S.A., Eremin V.F.,
Korneeva M.N., Nosik D.N., Rudneva I.A., Karamov E.V., Rytik P.G., Stakhanova V.M.,
Uryvaev L.V. /Analysis of sera by immunoblot method based on natural and recombinant HIV
antigen. // Problems of Virology.- 1988.-N.6.-P.700-703.
2. P.G.Rytik., G.van Der Groen., V.F. Eremin., N.D. Kolomietz., S.A.,Popov., P.Nys., V.
Willems., G. Vercauteren., Yu.G. Ilkevich., N.N.Lemeshko. /Biologic properties of HIV isolated
from an inhabitant in Byelorussia. // Zdravoohranenie Belorussii.- 1989.-N 11.-P.11-12.
3. P.G. Rytik., G.van der Groen., V.F. Eremin., N.D. Kolomietz., S.A. Popov., P. Nys., V.
Willems., G. Verkauteren., Yu.G. Ilkevich., N.N. Lemeshko. / Biological properties of HIV
isolated from a virus carrier in the USSR. // Problems of Virology.- 1990.-N.5.-P.389-390.
4. Eremin V.F. / Structural organization and intravirion interactions of bovine leukemia virus
proteins. // Experimental Oncology -1989.- V.11.- 1-35.
5. P.G. Rytik., N.D. Kolomietz., V.P. Luchko., R.I. Zharkevich., A.G. Kolomietz., Z.S.
Kravchuk., V.F. Eremin. /Clinic-laboratory observations of HIV-infection's cases. Proceedings
of the D.I.Ivanovsky Institute of Virology of the Academy of Medical Sciences of the USSR. "
Fundamental and Applied Aspects of AIDS.,Moscow., 1988.,P. 42-48.
6. A.G. Kolomietz., N.N. Poleschuk., V.F. Eremin ., et al. Especialites of patogenesis, diagnosis
and therapy CNS infections associated with herpes virus. Proceedings of the D.I. Ivanovsky
Institute of virology of the Academy of Medical Sciences of the USSR." Herpes"., Moscow.,
1990.,p.18-21.
7. P.G.Rytik., V.F. Eremin ., Z.B. Kvacheva .,N.N. Poleschuk., S.A. Popov., .C.Schroder.,
M.Bachman., B.E.Weiler., W.E.G Muller./Susceptibility of primary human glial fibrillary acidic
protein positive brain cells to human immunodeficiency virus infection in vitro: anti-HIV
activity of memantine.//AIDS-Research and Human Retroviruses.- 1991.-V.1.-P.89-94.
8. V.F. Eremin., S.A. Popov., I.I. Kutcherov., P.G .Rytik./A use of PCR for the controle of a
latent HIV - infection in vitro.// Problems of Virology.- 1991.-N.4.-p.331-332.
9. S.S. Marennikova., L.G. Stepanova., M.N. Nosik, P.G. Rytik., V.F. Eremin.,
G.R.Matsevich.,N.N.Yanova./Strain features of HIV-1 occuring in the USSR according to the
results of its study in cell cultures.// Problems of Virology. - 1991.- N.5.-p.356-361.
10. Werner E.G. Muller, Karin Pfeifer, Jock Forrest, Peter G. Rytik, Vladimir F. Eremin,
Sergei A.Popov, Heinz C.Schroder. /Accumulation of transcripts coding for prion protein in
human astrocytes during infection with human immunodeficiency virus. //Biochimica et
Biophysica Acta.- 1992.-1139.-p.32-40.
11.P.G.Rytik, Z.B. Kvachevaя1., V.F. Eremin., N.N. Poleschukя1., S.A. Popov., W.E.G.
Muller., D.M. Asher./A use of primary astrocites cell culture for modeling HIVneuroinfection.//J. Microbiol.Epidemiol.Immunol1.- 1993.- N1.- P.47-51.
12. Morphological diagnosis of a seronegative case of HIV-infection /M.K. Nedzved, V.F.
Eremin, I.I. Protas, B.F. Pochebyt //Arch. Patol.- 1995.- N4.- P.80-83.
13. Complex approach to the diagnosis of HIV-1 infection: use of polymerase chain reaction as
an alternative confirmation test / Eremin V.F., Popov S.A., Pyzhova N.S., Kucherov I.I.,
Kucherer K., Pauli G., Rytik P.G. //J. Microbiol. Epidemiol. Immunol.- 1996.-N5.-P.23-27.
14. Inhibition of the reproduction of human immunodeficiency virus ( HIV ) with cytokine-like
anti-virus factor / Gribkova N.V., Eremin V.F., Votyakov V.I., Shchelkanov M. Yu., Pashkova
T.A., Karamov E.V. //J. Microbiol. Epidem. Immunol. - 1996.-N5.-P.27-29.
15. Antibody to hepatitis E virus in HIV-infected individuals and AIDS patients / Balayan M.S.,
Fedorova O.E., Mikhailov M.I., Rytik P.G., Eremin V.F., Danilova T.I., Shevelev B.I.,
Gorbacheva E.C., Pankova G.Y. // J. Viral Hepatitis.- 1997.- N.4.- P.1-5.
16. HIV-I phenotype, chemokines, cytokines and its role in HIV-infection progression / Eremin
V.F., Votjakov V.I., Titov L.P. // J. Molecular Biology.- 1998, N6, P.783-789 (Rus.).
17. Phenotype characteristics of HIV isolates, isolated in the Belarus Republic / Eremin V.F //
Problems of Virology.- 1998, N3, P.113-117 ( Rus ).
18. Analysis of the immunologic heterogeneity of HIV-1: I. “Arginine-glutamine trigger” in the
fourth position of the central tetrapeptide of the V3 loop of gp 120 / Shchelkanov M.Iu.,
Iaroslavtseva N.G., Iudin A.N., Eremin V.F., Pyzhova N.S., Semiletov Iu.A., Abelian A.V.,
Karamov E.V. // Mol. Biol. (Mosk). – 1998. – V.4. – P. 717-728. ( Rus. ).
19. Analysis of the immunologic heterogeneity of HIV-1. II. Effect of amino acid residues,
flanking the C-terminus of the central tetrapeptide loop V3 gp 120 / Shchelkanov M.Iu.,
Iaroslavtseva N.G., Iudin A.N., Eremin V.F., Pyzhova N.S., Semiletov Iu.A., Abelian A.V.,
Karamov E.V. // Mol. Biol. (Mosk). – 1998. – V.4. – P. 729-734. ( Rus. ).
20. Extreme founder effect in an HIV type 1 subtype A epidemic among drug users in
Svetlogorsk, Belarus / Lukashov V.V., Karamov E.V., Eremin V.F., Titov L.P., Goudsmit J. //
AIDS Res. Hum. Retroviruses. – 1998. – V. 14. – P. 1299 – 1303.
21. Shchelkanov M.Iu., Iudin A.N., Eremin V.F., Gadynskaia N.I., Iaroslavtseva N.G., Karamov
E.V. / Increase in the serotypical heterogenety in HIV-1 in Svetlogorsk focus 1 year after the
start of an epidemic // Vopr. Virusol. - 1999. - V.4. - P.174-177; ( Rus. ).
22. Eremin V.F., Amvrosieva T.V., Votjakov V.I., Kvacheva Z.B., Diakonova O.V., Sharko
R.M., Titov L.P. / Use of rapid methods in detection of enteroviruses // Klin.Lab.Diagn. - 1999. V.3. - P.23-24, 33; ( Rus. ).
23. Shchelkanov M.Iu., Eremin V.F., Sakhuriya I.B., Burunova V.V., Pavlova T.V., Kornilaeva
G.V., Karamov E.V. / Dehydrogenase activity of infected cells and biological properties of HIV1 variants // Biochemistry - 1999. - V.4. - P.431-436. ( Rus. ).
24. Amvros'eva T.V., Titov L.P., Malders M., Hovi T., D'iakonova O.V., Votiakov V.I.,
Kvacheva Z.B., Eremin V.F., Sharko R.M., Orlova S.V., Kazinets O.N., Bogush Z.F. / Waterborne outbreak of serous meningitis caused by Echovirus-30 in Belarus // Zh Mikrobiol
Epidemiol Immunobiol. 2001 Jan-Feb;(1):21-5.
25. Eremin V.F., Barabanov L.G., Gasich E.L., Schimanskaja I.G., Dubiskaja G.P.,
/Serological, molecular biological diagnosis of STDs // Herald of dermatology and venerology //
2002.,-V.2., P.22-29.
26. Flekhter O.B., Boreko E.I., Tret'iakova E.V., Pavlova N.I., Baltina L.A., Nikolaeva S.N.,
Savinova O.V., Eremin V.F., Galin F.Z., Tolstikov G.A./ Synthesis and antiviral activity of
amides and conjugates of betulonic acid with amino acids // Bioorg Khim. 2004 JanFeb;30(1):89-98;
27. Flekhter O.B., Boreko E.I., Nigmatullina L.R., Pavlova N.I., Nikolaeva S.N., Savinova O.V.,
Eremin
V.F.,
Baltina
L.A.,
Galin
F.Z.,
Tolstikov
G.A.
/ Synthesis and antiviral activity of hydrazides and substituted benzalhydrazides of betulinic acid
and its derivatives // Bioorg Khim. 2003 May-Jun;29(3):326-32;
28. Lazouskaya N.V., Eremin V.F., Adema K.W., Gasich E.L., Baan E., Lukashov V.V. / The
HIV type 1 epidemic in Belarus: predominance of Eastern European subtype A strains and
circulation of subtype B viruses // AIDS Res Hum Retrov.-2005.-V.21.-P.830-833.
29. Christophe M. Olinger, Natallia V. Lazouskaya, Vladimir F. Eremin and Claude P. Muller/
Multiple genotypes and subtypes of hepatitis B and C viruses in Belarus: similarities with Russia
and West European influences // Clinical Microbiology and Infection J. – 2008.-V.14.- P.575581.
30. Distribution of Varizella-zoster virus (VZV) wild-type genotypes in northern and southern
Europe: evidence for high conservation of circulating genotypes // Loparev VN., Rubtcova EN.,
Bostik V., Tzaneva V., Sauerbrei A., Robo A.,Sattler-Dornbacher E., Hanovcova I.,Stepanova
V.,Splino M., EreminV., Koskiniemi M., Vankova O.E.., Schmid DS./ Virology- 2009.-V.383.P.216-225 .
31. Prevalence of high- and low-risk oncogenic human papillomaviruses in patients with external
genital pathology./Vergeichik GI, Stribuk ZhA, Eremin VF. //Vopr Virusol. 2011 MarApr;56(2):26-8. Russian.
32. A new unique recombinant HIV type 1 isolated from a child born to an HIV-infected
mother./ Eremin VF, Gasich EL, Sasinovich SV. // AIDS Res Hum Retroviruses. 2011
Dec;27(12):1323-6. doi: 10.1089/aid.2011.0112. Epub 2011 Jun 1.
33. HBV and HCV genotypes distribution on the territory of Belarus /Retrovirology, 2012,
9(Suppl 1). P.57 //Gasich E.L., Eremin V.F., Sasinovich S.V., Tulinova M.G.
34. A new unique recombinant HIV-1 revealed in Belarus / Retrovirology, 2012, 9(Suppl 1).
P.32 // Eremin VF., Gasich EL., Sasinovich SV;
35. Transmitted HIV drug resistance (tHIVDR) in treatment naïve patients in Belarus /Reviews
in Antiviral Therapy & Infectious diseases, 2012.-V.2. P.42-43 (Abst.17) // Ilyenkova, V
Eremin, I. Eramova, E.Gasich, S.Sasinovich;
36. Molecular epidemiology of genital papillomavirus infection in the Republic of Belarus
/.Vergeĭchik GI, Eremin VF. / Zh Mikrobiol Epidemiol Immunobiol. 2012 Mar-Apr;(2):55-60.
Russian.
37. The HIV-1 resistance mutations revealed at patients on HAART in Belarus / Antibiotic
resistance and infection control (ARIC), European Conference, 17th November, 2011, Kaunas,
Lihuania p.27// Eremin V.F., Gasich E.L.,Sasinovich S.V., Suetnov O.N., Gorbunova N.A.,
Anbarzumjan E.G., Iljenkova V.S.,Grushko T.P., Grushko P.N., Vasilenko A.I., Karpov I.A.
38. HIV/AIDS molecular epidemiology in Belarus, 2008-2011 / Antibiotic resistance and
infection control (ARIC), European Conference, 17th November, 2011, Kaunas, Lihuania p.42 //
Eremin V.F., Gasich E.L., Sasinovich S.V., Suetnov O.N., Grushko T.P., Grushko P.N.,
Iljenkova V.S.,Gorbunova N.A., Karpov I.A.
39. Andrianov, A.M., Kornoushenko, Y.V., Anishchenko, I.V., Eremin, V.F., Tuzikov, A.V.
Structural analysis of the envelope gp120 V3 loop for some HIV-1 variants circulating in the
countries of Eastern Europe // Journal of Biomolecular Structure and Dynamics - 2013 –
V.31 (7) PP. 665 - 683 Cited 2 times.
40. A New Unique Recombinant HIV Type 1 Isolated from a Child Born to an HIV-Infected
Mother/ Eremin VF, Gasich EL, Sasinovich SV. // AIDS Res Hum Retroviruses. 2011
Dec;27(12):1323-6.
41. Prevalence of high- and low-risk oncogenic human papillomaviruses in patients with external
genital pathology./Vergeichik GI, Stribuk ZhA, Eremin VF. // Vopr Virusol. 2011 MarApr;56(2):26-8. Russian.
42. Molecular epidemiology of genital papillomavirus infection in the Republic of Belarus /
Vergeĭchik GI, Eremin VF. / Zh Mikrobiol Epidemiol Immunobiol. 2012 Mar-Apr;(2):55-60.
Russian.
43. Pereslavtseva, A.V., Tolmacheva, I.A., Slepukhin, P.A., El'Tsov, O.S., Kucherov,
I.I., Eremin, V.F., Grishko, V.V. / Synthesis of A-Pentacyclic Triterpene α,β-Alkenenitriles
// Chemistry of Natural Compounds – 2014.- 49 (6) PP. 1059 - 1066
44. Eremin, V., Gasich, E., Sasinovich, S., Suetnov, O., Kucherov, I. / HIV drug resistance at
patients on HAART and transmitted HIV drug resistance (tHIVDR) in treatment naive patients
in Belarus // Journal of AIDS and Clinical Research -2013.- V.4 (12) doi: 10.4172/21556113.1000258
45. Tolmacheva, I.A., Igosheva, E.V., Vikharev, Yu.B., Grishko, V.V., Savinova, O.V., Boreko,
E.I., Eremin, V.F. / Synthesis and biological activity of mono- and diamides of 2,3secotriterpene acids // Russian Journal of Bioorganic Chemistry 2013. -39 (2) PP. 186 - 193
46. Tolmacheva, I.A., Igosheva, E.V., Vikharev, I.B., Grishko, V.V., Savinova, O.V., Boreko,
E.I., Eremin, V.F. / [Synthesis and biological activity of 2,3-secotriterpene acid mono- and
diamides]. // Bioorganicheskaia khimiia – 2013. - 39 (2) PP. 212 - 220
47. Andrianov, A.M., Anishchenko, I.V., Kisel, M.A., Kornoushenko, Y.V., Nikolayevich,
V.A., Eremin, V.F., Kucherov, I.I., Tuzikov, A.V. / Computer-aided design of novel HIV-1 entry
inhibitors blocking the virus envelope gp120 V3 loop // Biopolymers and Cell - 2012 28 (6). PP. 468 - 476
48. Igosheva, E.V., Tolmacheva, I.A., Vikharev, Yu.B., Grishko, V.V., Savinova, O.V., Boreko,
E.I., Eremin, V.F. / Synthesis and biological activity of amides of 28-methoxy-28-oxo-1-cyano2,3-seco-lup-20(29)-en-3-oic acid // Chemistry of Natural Compounds -2012. - PP. 1 - 6
49. Igosheva, E.V., Tolmacheva, I.A., Vikharev, Yu.B., Grishko, V.V., Savinova, O.V., Boreko,
E.I., Eremin, V.F. Synthesis and biological activity of amides of 28-methoxy-28-oxo-1-cyano-2,
3-seco-lup-20(29)-en-3-oic acid // Chemistry of Natural Compounds - 2012 – V. 48 (3) PP.
426 – 431.
50. Eremin, V.F., Gasich, E.L., Sosinovich, S.V. / A new unique HIV-1 recombinant form
detected in Belarus // Voprosy Virusologii - 2012 57 (3) PP. 9 - 13
51. Eremin, V.F., Gasich, E.L., Sasinovich, S.V. / A new unique recombinant HIV type 1
isolated from a child Born to an HIV-infected mother // AIDS Research and Human
Retroviruses -2011.- V.27 (12) PP. 1323 - 1326
Business Address: RRPCEM, 23 Filimonova st., Minsk, 220114, Belarus