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Effects of combined chelation treatment with pyridoxal isonicotinoyl hydrazone analogs and deferoxamine in hypertransfused rats and in iron-loaded rat heart cells by Gabriela Link, Prem Ponka, Abraham M. Konijn, William Breuer, Z. Ioav Cabantchik, and Chaim Hershko Blood Volume 101(10):4172-4179 May 15, 2003 ©2003 by American Society of Hematology Structure of pyridoxal isonicotinoyl hydrazone (PIH) and its analogs.These compounds were synthesized by Schiff base condensation as described previously,12 and the absence of significant amounts of the starting materials in these preparations was confirmed... Gabriela Link et al. Blood 2003;101:4172-4179 ©2003 by American Society of Hematology Effects of 108-o dose.Effect on radioiron excretion (A) and on residual hepatic radioactivity (B) with (■) or without (▪) the addition of a constant (100 mg/kg) dose of DFO. Results are expressed as net 7-day measurements (percentage of injected radioactivi... Gabriela Link et al. Blood 2003;101:4172-4179 ©2003 by American Society of Hematology Effect of treatment on radioiron uptake by cultured heart cells.Five-day-old cultures were incubated with 160 μM59Fe-labeled ferric ammonium citrate for 24 hours in the presence of PIH analogs at concentrations ranging from 0 to 160 μM iron-binding equivale... Gabriela Link et al. Blood 2003;101:4172-4179 ©2003 by American Society of Hematology Effect of treatment on radioiron mobilization from cultured iron-loaded heart cells.Five-day-old cultures were incubated with 160 μM. Gabriela Link et al. Blood 2003;101:4172-4179 ©2003 by American Society of Hematology Effect of increasing PIH analog and DFO concentrations on radioiron mobilization from cultured, iron-loaded heart cells.DFO ± analog 1: Molar concentration of mixed DFO + analog was identical to the sum of the corresponding single drugs—that is, it was twic... Gabriela Link et al. Blood 2003;101:4172-4179 ©2003 by American Society of Hematology Dose-response curves.Dose-response curves of chelator 108 in the absence (○) and presence (●) of 20 μM (left) or 40 μM (right) deferoxamine. Gabriela Link et al. Blood 2003;101:4172-4179 ©2003 by American Society of Hematology Effect of 108-o concentration on iron exchange between 108-o-Fe and fluorescein-DFO (FlDFO).Complexes of 108-o:Fe were preformed by mixing Fe:NTA (5 mM ferrous ammonium sulfate/35 mm nitrilotriacetate) with 108-o in HBS to yield solutions containing 10 μM ... Gabriela Link et al. Blood 2003;101:4172-4179 ©2003 by American Society of Hematology
