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Versatile Bioassays Using Surface Plasmon Resonance Xiaodi Su Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), Singapore [email protected] Surface Plasmon Resonance (SPR) spectroscopy from a planar gold film is an important technique for studying biomolecular interactions on solid-liquid interface. Noble metal nanoparticles, i.e. gold nanoparticles (AuNPs), have strong interactions with light to generate localized SPR (LSPR) that leads to unique optical properties. We have developed versatile SPR assay protocols and AuNPs-based bioassays for interrogating biomolecular interactions concerning transcription factors, DNA, small molecular drugs, DNA aptamer, peptide nucleic acids, and antibody. Complementary surface sensitive techniques, i. e. quartz crystal microbalance (QCM) and dual polarization interferrometry (DPI), have been coupled with SPR measurement for comprehensive understanding of biological binding events. AuNPs’ particle distancedependent colorimetric property and super quenching to fluorophores have been exploited to meet the sensitivity of specific applications. Through parallel study of the same biological binding events using planar gold-based SPR and nano gold-based LSPR we have compared the performance of the solid-liquid phase and homogenous phase SPR assays and can comment on their utilities for fundamental research and for on-site applications.
