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Name_________________________________________________Per._____________Date___
Enzyme Experiment Research Plan
Due Date:_____________Dec. 9th, Tues______________
Read the following directions carefully
Your Research Plan/Results and Conclusions should be in the following format
Read all sections-A through G! Proofread and do not wait until the last minute!
Format: 12 font New Times Roman or Ariel, double spaced, centered headings, spelling
correct. (each section also has individual instructions).
A. QUESTION BEING ADDRESSED
What is the question you are trying to answer by doing your research? (this is
something that you can only answer after doing some background research) For this
assignment you should research the following questions: What type of molecule is an
enzyme? Explain how they function (include terms: substrate, active site, products,
activation energy, biological catalyst. . Describe the purpose of the enzyme Catalase in
living things. Explain how hydrogen peroxide is produced in living things and it’s
function in living things. Describe at least 3 things that could influence enzyme action.
This part of the assignment should be headed BACKGROUND RESEARCH, typed
according to the correct format, and have the Bibliography also. There are no minimum
number of sources (1 if fine if it is good), so citations are only necessary if you have more
than 1. This should be 150-250 words (1/2 to 1 page only!).
B. HYPOTHESIS
What is the hypothesis? Use an If…., then…. format. This should include your
Independent and Dependent Variable you will be using in your lab.
C. MATERIALS
The material list should be in the following bulleted format and follow the guidelines
listed below.




Items are not numbered
Size and quantity of each item listed
Specific names and/or scientific names are used
All measurements in metric units
(For this lab, we will give you the possible materials, if you use something different
you must add it.)
Your available materials:
3% Hydrogen Peroxide
Test tubes, beakers, graduated cylinders, droppers
Filter paper
Raw carrot, potato, calf liver (if you use another substance it must be listed)
Ice
Hotplate
Acid solution
Base solution
Morter and pestle (for crushing)
Blender
Distilled water
With a partner, after doing background research, you will design your own
experiment using the above materials ( you do not have to use them all, but
you must have a source of catalase and you must at least use hydrogen
peroxide). You will write a very detailed step by step procedure according to
the directions below.
D. PROCEDURES
The procedure should be written in the following format and follow the guidelines:
1. Steps should be numbered.
2. Each action is written as a different step
Ex.
1. Fill 100 ml beaker with 25 mL of H2O2.
2. Measure 25 ml of catalase with a 50 ml graduated cylinder.
3. Pour the 25 ml of H2O2 into the 100 ml beaker, NOT IN PARAGRAPH
FORM.
3. It is written so that another student or researcher could exactly reproduce the
experiment.
4. Steps include all equipment used in the experimental set-up.
5. Steps include an exact description of how measurements will be taken and tools
that will be used
6. Steps are written in the 3rd person. Do not write your procedure in 1st person
(no I, We, You)
7. Include enough repeated trials in the procedure, this will depend on your
particular experiment, more is better (15 trials is the minimum)
8. Identify a control in the steps.
9. Describe constants and plan for uniform conditions for all trials
E. DATA ANALYSIS
You need to describe how you will analyze your data. You will use descriptive and
inferential statistics to evaluate the validity of your data. Write a statement to this effect.
“Descriptive and inferential statistics will be used to evaluate the data.”
DUE DATE:
Points
Possible
Question/Short Background Research
This is in the correct format and a question that can be
answered by the experiment.
Format
Paper is written in the correct format, no spelling or grammar
issues, correct APA format, font size and type, Headings are
centered.
Hypothesis
This is in an If…, then…. format? It addresses both the IV and
DV.
15
5
5
Materials
Items not numbered (bullet points), size and number of each
item listed, specific names and/or scientific names used,
measurements in Metrics, all necessary equipment is included,
ordering information if included
10
Procedures
Numbered steps, each action a different step, can be
reproduced, specific equipment listed in steps, written in 3rd
person, repeated trials, control included and described,
constants and uniform conditions described
25
Total
60
Points
Earned