Download Increase your protein yield with Nmr

Increase your protein yield with Nmr-Bio rich labeling media
for the production of [1H,13C]-Methyl, U-[D] proteins.
The use of NMR-Bio rich labeling media
is a cost effective solution to :
• Enhance protein yield • Increase protein solubility
• Speed-up protein production
• Produce protein libraries in parallel
• Simplify purification
Cell growth in D2O using
Production of a 37 kDa U-[D] protein 13CH -Alaβ,
3
Nmr-Bio medium
3
MW
kDa
M9
M9/D2O
medium
Ins.
O.D. (600 nm)
Due to the toxicity of D2O, the yield of many
valuable protein targets, overexpressed in
D2O/M9 media, is considerably reduced
compared to standard H2O/LB based cultures.
Furthermore, many interesting proteins are
recovered in inclusion bodies when produced
in perdeuterated M9 medium, requiring
complex refolding steps.
Sol.
Nmr-Bio
medium
Ins.
Sol.
116
2
Increase soluble proteins
66
45
1
35
25
100
300
500
Time (min)
Reduce proteins in inclusion bodies
An increase of protein yield by a factor of 3 to
10 has been reported by NMR-bio kits users.
NMR-Bio–RLM rich labeling media are supplemented with perdeuterated 12C-carbon and 15N-nitogen
sources and are calibrated for direct addition into pure M9 D2O culture medium (w/o glucose &
ammonium chloride). The version which is nitrogen and carbon sources-free (NMR-Bio–RLC ) is also
available to respond to your specific need! Please visit our booth for more information.
Prices/volume of culture Examples of NMR-Bio
13CH
3
rich labeling media
SLAM–RLC Rich labeling complement for single labeled methyl
group (does not contain 15N and 12C sources)
DLAM-RLC
Rich labeling complement for double labeled methyl
groups (does not contain 15N and 12C sources)
TLAM–RLC
Rich labeling complement for triple labeled methyl
groups (does not contain 15N and 12C sources)
groups
Labeled
Single methyl group (Aβ or Mε or Tγ)
Two methyl groups combination
(Aβ / Mε or Aβ / Tγ or Mε / Tγ )
Three methyl groups combination
(Aβ / Mε / Tγ)
SLAM–RLM
Single methyl group Rich labeling medium for single labeled methyl
group (including U-[2H] 15N and 12C sources)
(Aβ or Mε or Tγ)
DLAM-RLM
Two methyl groups combination
Rich labeling medium for double labeled methyl
groups (including U-[2H] 15N and 12C sources)
(Aβ / Mε or Aβ / Tγ or Mε/ Tγ )
TLAM–RLM
Three methyl groups combination
Rich labeling medium for triple labeled methyl
groups (including U-[2H] 15N and 12C sources)
(Aβ / Mε / Tγ)
0.25 L
0.5 L
1 L
390 € 590 €
990 €
520 € 790 €
1290 €
590 € 890 €
1500 €
650 €
990 €
1600 €
720 €
1090 €
1800 €
790 €
1190 €
2000 €
* Prices in Euro (€) are converted into USD ($) and Yen (¥) according to the currency exchange rate corresponding to the order date. The listed prices
exclude shipping fees and importation tax. Discounts apply for larger quantities. For specific quote contact us at [email protected]. Visit Nmr-Bio booth and get 10% discount
on quotes issued during the conference !
More than 100 kits available online!
[email protected]
– RESEARCH USE ONLY – PRINTED IN FRANCE – 06/2015 –