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BIOL 201 - Assignment 4 - Due April 3, 2008
How differences in animal cell division are regulated
EK Kieserman et al. (2008) Developmental regulation of central spindle assembly and
cytokinesis during vertebrate embryogenesis. Current Biology 18:116-123
You may do this assignment by yourself or in groups of two or three. You may
discuss this assignment with anyone you want but when writing this assignment only
people listed as authors may be present. Your answers must be in your own words and
not paraphrased from the article or from other sources. When writing your answers
consider myself to be your audience. Assume that I am familiar with cell biology
techniques and information but that I read this paper a year ago and have forgotten the
details. Cite any sources used other than the article itself. Your assignment is to be
single sided, use a cover page, and not be enclosed within a folder. You are
encouraged to use hand drawn diagrams rather than computer drawn ones. This
assignment is out of ****** marks and is worth 7.5% of your total course mark.
Question 1 Figure 2D shows a cell in anaphase. Draw what the figure would have
looked like if the authors had used antibodies against DNA and (i) Actin, (ii) Myosin, (iii)
intermediate filaments, (iv) Condensin, and (v) Pom121. Use one diagram for each.
(5 marks)
Question 2 In a short paragraph describe the experiments shown in Figures 1A, 1B,
and 1C and movies S1 and S2. Your answer must be in your own words and not those
of the authors. Include in your answer the purpose of this experiment, how was it done,
the results, and the conclusion. (5 marks)
Question 3 Figures 2A-B and C-J look similar but there are two major differences in
how these images were obtained. What are they? (2 marks)
Question 4 In a short paragraph describe the experiment shown in Figures 3D and 3E
and movies S9 and S10. Your answer must be in your own words and not those of the
authors. Include in your answer the purpose of this experiment, how was it done, the
results, and the conclusion. (5 marks)
Question 5 Design an experiment to determine whether the amount of PRC1 has an
affect on yeast mitosis. (Assume that yeast mitosis is regulated by PRC1 as well.) Your
experiment should use a fluorescent microscopy base approach. You have access to
any protein, cell type, reagent, or laboratory equipment you want. Include controls in
your experiments and how you would interpret the results. Include a labeled diagram of
possible results with your answer. (5 marks)
BIOL 201 - Assignment 4
Due: April 3, 2008
Your name(s) here