Download Requirements for Customized Transgenic Cell Line

Custom CRISPR Cell Line Service
Instructions
1. Please complete and email this form to [email protected]
2. Our Account Manager will contact you with a quote.
Customer Information
If you have registered an account with SBI, you can just identify yourself by giving us your name and email
address or Account No.
Name:
Account No.:
Phone:
Organization:
Shipping Address:
Email Address:
Genomic Editing
□Knockout or □Knockin/Mutation by Design
(If knockin/mutation by design service is required, please fill out knockin/mutation by design
section on page 3)
Suggested method for transfection
□Viral □Chemical transfection, please specify the reagent:_________________
□Electroporation □Nucleofection
Name of Target Gene (NCBI Accession Number):
DNA Sequence for Targeting:
Does KO/KI of the target gene affect cell growth?
□Yes □No □Not sure
If yes, how to rescue the KO/KI clones:__________________________________
Does KO/KI of the target gene affect cell survival?
□Yes
□No
□Not sure
If yes, how to rescue the KO/KI clones:__________________________________
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Host Cell Line
Name of Host Cell Line:
Who provides host cell line?
□SBI (Note: Extra fee will be charged) □Client(Note:Mycoplasma free certificate will be
required)
Growth condition of host cell line?
□Adherent
□Suspension
□Both
Please provide cell doubling time:____________________________________
Resistance of host cell line?
□G418□Puromycin □Zeocin □Hygromycin B □Blasticidin S □Other___________
□No resistance.
Will serial dilution affect cell growth rate?
□Yes
□No
□Not sure
What are the medium and additives for cell growth?
Medium:______________________________________________________
Additives:_____________________________________________________
Do you need SBI to follow any special cell culture routine? □Yes, see below
□No
Please provide protocol with information about the cell line and any special growth characteristics
or requirements.
Do the cells contain any human pathogen?
□Yes, please specify:___________________________ □No
Requirements for Customized Transgenic Cell Line:
Number of allele(s) to be modified:
□Single allele
□Two alleles
□Multiple alleles (indicate number)_________
Please indicate the preferred type of analysis to characterize transgenic cell line (Extra fees
may be charged):
□Growth curve
□Western blot(antibodies to be provided) □Not required
What is the final application of the transgenic cell line?
□Gene function analysis
□Assay development
□Drug screening
□Other
If Other, Please indicate your specific application and requirements:____________________
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Comments:
Gene Name for Knockin/Mutation by Design:
Gene Accession Number:
For
Knockin/Mutation
by Design Services
DNA Sequence:
Reporter/Tag:
N- or C-terminal:
Selection Marker:
Project Information
Is this project for grant application purpose?
□Yes□No
When will the project start?
□Immediately□Within one month□Within three months□Half a year later
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