Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download View/Open - Repository Unhas
Document related concepts
DNA vaccination wikipedia , lookup
Transmission (medicine) wikipedia , lookup
Neonatal infection wikipedia , lookup
Infection control wikipedia , lookup
Childhood immunizations in the United States wikipedia , lookup
Common cold wikipedia , lookup
Human cytomegalovirus wikipedia , lookup
Marburg virus disease wikipedia , lookup
Transcript
Detection of viruses from feces of tiger shrimp (Penaeus monodon) broodstocks using multiplex PCR Hilal Anshary1, Sriwulan1 and Sukenda2 1) Laboratory of Fish Parasites and Diseases, Department of Aquaculture, Faculty of marine science and fisheries, Hasanuddin University (Contact person: Hilal Anshary: E-mail, [email protected]) 2) Departemen of Aquculture, Bogor Agricultural University Abstract The purpose of this research was to apply a non-detrimental technique to screen shrimp broodstocks for virus infection. Individual or pooled feces samples were collected and analyzed using multiplex PCR. The total of 125 and 75 broodstocks from Bombana and Aceh, respectively, were examined for viruses. Genomic DNA of the shrimp feces were extracted following the manual of QiaAmp DNA stool extraction kit. We were able to detect WSSV, IHHNV and MBV from the broodstocks feces. MBV and IHHNV were frequently found. This finding suggests that broodstocks may function as source of virus infection in growout ponds through infected seeds. Key words: broodstocks, feces, multiplex PCR, tiger shrimp, virus
