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An endocrine disrupting chemical, octylphenol, resulted in the inhibition of
mesenchymal stem cell growth derived from umbilical cord blood via alteration
of cell-cycle related genes [14 font]
Ryeo-Eun Go1, Seung-Hee Kim1, David Smith2 and Kyung-Chul Choi 1,* [12 font bold]
1
Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk
National University, Cheongju, Chungbuk, Republic of Korea [12 font italic]
2
Author Affiliation, Country [12 font italic]
Umbilical cord blood is defined as a blood that exists in the placenta and in the attached
umbilical cord after childbirth. Recently, cord blood has been considered for research because
it contains mesenchymal stem cells (MSCs), stromal cells which have multipotency that
allow them to differentiate into a variety of cell types. Among endocrine disrupting chemicals
(EDCs), octylphenol (OP) is one of the alkylphenols used widely in the industry, which have
lead to human health problems such as reproductive abnormality. In this study, we isolated
human MSCs derived from umbilical cord blood and showed that cultured MSCs were shown
to express surface marker of CD34, but did not express CD105. We further examined the
effect of OP on human umbilical cord blood through examination of MSCs following
exposure to OP by a cell proliferation assay, RT-PCR and Western blot analysis. It was shown
that both transcriptional and translational levels of cyclin D1 were induced, while p21 level
was suppressed by OP compared to a negative control in these MSCs. This collapse of cell
cycle regulation by OP directly stimulated MSC growth, suggesting that the disregulation of
cell cycle by OP in these MSCs were derived from umbilical cord blood. These results may
help elucidate the action of common EDCs on MSCs from human umbilical cord blood.
However, further study is required to determine signaling pathway related to the effects of
EDCs in MSCs. [limit 300 words; 12 font; no figures nor tables]
Keyword: Endocrine disrupting chemicals, octylphenol, mesenchymal stem cells [at least 3]
Funding Source: This work was supported by a grant (no. PJ009599) from the NextGeneration BioGreen 21 Program, Rural Development Administration, Republic of Korea.
Disclosure Statement: None of the authors have any conflicts of financial interest to declare.
* Correspondence to : [email protected]