Download Zinc Finger Consortium Expression Vector and Modular

Zinc Finger Consortium Expression
Vector and Modular Assembly Kit
Description: The Zinc Finger Consortium (www.zincfingers.org) was
established to promote continued research and development of
engineered zinc finger technology. Consortium members Keith Joung
and Daniel Voytas have deposited their Expression Vector Kit and
Modular Assembly Kit at Addgene. Detailed information can be found at
www.addgene.org/zfc.
References:
1. Standardized reagents and protocols for engineering zinc finger
nucleases by modular assembly. Wright et al., Nature Protocols 1,
1637-1652 (2006).
2. Unexpected failure rates for modular assembly of engineered zincfingers. Ramirez, et al., Nature Methods 5, 374-375 (2008).
Expression Vector Kit v1.0:
* pGP-FF
* pGP-FB-orig BA (positive control)
* pAC-Kan-alphaGal4
* pBAC-lacZ
* pBAC-BA-lacZ (positive control)
* KJBAC1 bacterial strain
* pST1374 (mammalian expression vector)
* pDW1775 (plant expression vector)
Modular Assembly Kit v1.0:
The 141 pc3XB-based zinc finger plasmids are divided into two plates.
Please visit www.addgene.org/zfc to browse zinc finger plasmids by
target DNA sequence and finger amino acid sequence, to obtain a tab
delimited data file, and for detailed plasmid information.
Handling and Storage:
freeze-thaw cycles.
Store glycerol stocks at -80oC and minimize
Note: These plasmid constructs are being distributed to non-profit
institutions for the purpose of basic research. Please contact Keith
Joung or Daniel Voytas to determine proper attribution on any
publications arising from the use of these plasmids.
Please contact Addgene at [email protected] with any questions.
www.addgene.org
[email protected]
Zinc Finger Consortium Expression
Vector and Modular Assembly Kit
Plate 1
A
B
C
D
E
F
G
H
1
ZF1
ZF13
ZF25
ZF37
ZF49
ZF61
ZF73
ZF85
2
ZF2
ZF14
ZF26
ZF38
ZF50
ZF62
ZF74
ZF86
3
ZF3
ZF15
ZF27
ZF39
ZF51
ZF63
ZF75
ZF87
4
ZF4
ZF16
ZF28
ZF40
ZF52
ZF64
ZF76
ZF88
5
ZF5
ZF17
ZF29
ZF41
ZF53
ZF65
ZF77
ZF89
6
ZF6
ZF18
ZF30
ZF42
ZF54
ZF66
ZF78
ZF90
7
ZF7
ZF19
ZF31
ZF43
ZF55
ZF67
ZF79
ZF91
8
ZF8
ZF20
ZF32
ZF44
ZF56
ZF68
ZF80
ZF92
9
ZF9
ZF21
ZF33
ZF45
ZF57
ZF69
ZF81
ZF93
10
ZF10
ZF22
ZF34
ZF46
ZF58
ZF70
ZF82
ZF94
11
ZF11
ZF23
ZF35
ZF47
ZF59
ZF71
ZF83
ZF95
12
ZF12
ZF24
ZF36
ZF48
ZF60
ZF72
ZF84
ZF96
Plate 2
A
B
C
D
E
F
G
H
1
ZF97
ZF109
ZF121
ZF133
2
ZF98
ZF110
ZF122
ZF134
3
ZF99
ZF111
ZF123
ZF135
4
ZF100
ZF112
ZF124
ZF136
5
ZF101
ZF113
ZF125
ZF137
6
ZF102
ZF114
ZF126
ZF138
7
ZF103
ZF115
ZF127
ZF139
8
ZF104
ZF116
ZF128
ZF140
9
10
11
12
ZF105 ZF106 ZF107 ZF108
ZF117 ZF118 ZF119 ZF120
ZF129 ZF130 ZF131 ZF132
ZF141
13480 13420 13421 13422 13423 13424 13426 13456
Expression Vectors in Plate 2, Row F:
Plasmid ID and Name
13480: pGP-FF
13420: pGP-FB-orig BA
13421: pAC-Kan-alphaGal4
13422: pBAC-lacZ
13423: pBAC-BA-lacZ
13424: KJBAC1 strain
13426: pST1374
13456: pDW1775
Antibiotic Resistance
Ampicillin
Ampicillin
Kanamycin
Chloramphenicol
Chloramphenicol
Tetracycline
Ampicillin
Ampicillin
Instructions: To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette
tip, puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar
plate. To patch the hole, use sterile tape or a portion of a fresh aluminum seal.
Please visit www.addgene.org/zfc for plasmid information.