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Zinc Finger Consortium Expression Vector and Modular Assembly Kit Description: The Zinc Finger Consortium (www.zincfingers.org) was established to promote continued research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas have deposited their Expression Vector Kit and Modular Assembly Kit at Addgene. Detailed information can be found at www.addgene.org/zfc. References: 1. Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Wright et al., Nature Protocols 1, 1637-1652 (2006). 2. Unexpected failure rates for modular assembly of engineered zincfingers. Ramirez, et al., Nature Methods 5, 374-375 (2008). Expression Vector Kit v1.0: * pGP-FF * pGP-FB-orig BA (positive control) * pAC-Kan-alphaGal4 * pBAC-lacZ * pBAC-BA-lacZ (positive control) * KJBAC1 bacterial strain * pST1374 (mammalian expression vector) * pDW1775 (plant expression vector) Modular Assembly Kit v1.0: The 141 pc3XB-based zinc finger plasmids are divided into two plates. Please visit www.addgene.org/zfc to browse zinc finger plasmids by target DNA sequence and finger amino acid sequence, to obtain a tab delimited data file, and for detailed plasmid information. Handling and Storage: freeze-thaw cycles. Store glycerol stocks at -80oC and minimize Note: These plasmid constructs are being distributed to non-profit institutions for the purpose of basic research. Please contact Keith Joung or Daniel Voytas to determine proper attribution on any publications arising from the use of these plasmids. Please contact Addgene at [email protected] with any questions. www.addgene.org [email protected] Zinc Finger Consortium Expression Vector and Modular Assembly Kit Plate 1 A B C D E F G H 1 ZF1 ZF13 ZF25 ZF37 ZF49 ZF61 ZF73 ZF85 2 ZF2 ZF14 ZF26 ZF38 ZF50 ZF62 ZF74 ZF86 3 ZF3 ZF15 ZF27 ZF39 ZF51 ZF63 ZF75 ZF87 4 ZF4 ZF16 ZF28 ZF40 ZF52 ZF64 ZF76 ZF88 5 ZF5 ZF17 ZF29 ZF41 ZF53 ZF65 ZF77 ZF89 6 ZF6 ZF18 ZF30 ZF42 ZF54 ZF66 ZF78 ZF90 7 ZF7 ZF19 ZF31 ZF43 ZF55 ZF67 ZF79 ZF91 8 ZF8 ZF20 ZF32 ZF44 ZF56 ZF68 ZF80 ZF92 9 ZF9 ZF21 ZF33 ZF45 ZF57 ZF69 ZF81 ZF93 10 ZF10 ZF22 ZF34 ZF46 ZF58 ZF70 ZF82 ZF94 11 ZF11 ZF23 ZF35 ZF47 ZF59 ZF71 ZF83 ZF95 12 ZF12 ZF24 ZF36 ZF48 ZF60 ZF72 ZF84 ZF96 Plate 2 A B C D E F G H 1 ZF97 ZF109 ZF121 ZF133 2 ZF98 ZF110 ZF122 ZF134 3 ZF99 ZF111 ZF123 ZF135 4 ZF100 ZF112 ZF124 ZF136 5 ZF101 ZF113 ZF125 ZF137 6 ZF102 ZF114 ZF126 ZF138 7 ZF103 ZF115 ZF127 ZF139 8 ZF104 ZF116 ZF128 ZF140 9 10 11 12 ZF105 ZF106 ZF107 ZF108 ZF117 ZF118 ZF119 ZF120 ZF129 ZF130 ZF131 ZF132 ZF141 13480 13420 13421 13422 13423 13424 13426 13456 Expression Vectors in Plate 2, Row F: Plasmid ID and Name 13480: pGP-FF 13420: pGP-FB-orig BA 13421: pAC-Kan-alphaGal4 13422: pBAC-lacZ 13423: pBAC-BA-lacZ 13424: KJBAC1 strain 13426: pST1374 13456: pDW1775 Antibiotic Resistance Ampicillin Ampicillin Kanamycin Chloramphenicol Chloramphenicol Tetracycline Ampicillin Ampicillin Instructions: To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip, puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate. To patch the hole, use sterile tape or a portion of a fresh aluminum seal. Please visit www.addgene.org/zfc for plasmid information.