Download Supplementary Figure S1 Supplementary Figure S1: Modulation of

Supplementary Figure S1
Supplementary Figure
S1: Modulation of anticancer drug sensitivity of NRK-52E cells by Harpagophytum
procumbens extract.
A-C: Logarithmical growing NRK-52E cells were pre-treated for 24 hours with H. procumbens
extract (0.02 mg/ml). Afterwards, cisplatin was added at the indicated concentrations. After
further incubation period of 48 hours cell viability (A) and cell cycle distribution (B) were
determined. A, mean ± SEM from n = 1-2 independent experiments each performed in
quadruplicate; B, representative data obtained from FACS analysis. C: Microscopical
examination of cisplatin treated cells that have been pre-treated (+HAP) or not (-HAP) with H.
procumbens extract.
D: Rat tubular kidney cells (NRK-52E) were pre-treated for 24 hours with H. procumbens extract
(0.02 mg/ml) (HAP) before cisplatin (10 µM or 30 µM) (CisPt) was added. After further
incubation period of eight hours, the number of nuclear H2AX foci/cell, which are indicative of
the DDR stimulated by DNA double-strand breaks (DSBs), was analyzed by
immunohistochemistry as described in methods. The upper part of the figure shows a
representative picture. In the histogram below the median as well as the 95 % and 5 %
percentiles and quartiles are shown. * p≤0.05.
E: Logarithmical growing rat tubular kidney cells (NRK-52E) were pre-treated for 24 hours with
crude extract from H. procumbens (0.01 mg/ml). Afterwards doxorubicin was added. After postincubation period of 48 hours cell viability was determined. Data shown are the mean ± SEM
from n = 3-4 independent experiments each performed in quadruplicate.