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Domestication & Agriculture • Paleolithic people (250,000-10,000 yrs. Ago) • hunter-gatherers • move to follow migrating animals or find new game • ate edible plants (i.e. wheat, barley) as they traveled Agrarian Societies Developed • 10,000 years ago • Far East, Europe, Egypt (Fertile Crescent) • no longer nomadic • domesticated plants (wheat, barley, rye) & animals (goats, sheep, cattle, dogs for milk, cheese, butter, meat) • No longer nomadic • permanent settlements now possible • more “leisure” time meant opportunities to develop/invent language, art, technology Origins of Biotechnology • Plants - saved seeds from plants with most desirable traits & planted them the following year • Animals – only the best were allowed to breed • new varieties of plants and animals arose through manipulation of genetic inheritance – known as artificial selection or selective breeding. • Seeds, cuttings, & animals used for barter • increased distribution of desirable genes between farms, communities, & continents • High tech seeds & crops used by farmers. • Wild & ancient varieties being lost, genetic diversity decreasing. • Gene Banks set up to save ancient “germplasm” Present Day • With stationary lifestyle came need for food storage. • Fermentation decreases spoilage, often enhances flavor, texture. Fermented Foods Fermentation of Glucose • Above equation shows cellular respiration when O2 is plentiful. When oxygen is limited, cells that would have turned sugar into CO2, water, and ATP instead convert it into CO2 and other compounds like alcohol or lactic acid. • Unleavened (flat) bread - made with milled flour, water, & salt, then baked Leavened Bread • Probably discovered by accident • Yeast contaminated stored dough, fermented, then was baked. • CO2 bubbles cause fluffy texture • Starter - old, fermented, uncooked dough mixed with fresh batch of dough - no need for chance contamination. • Process brought from Egypt to Greece & Rome, then from Rome to conquered lands. • Baker’s yeast - strain of yeast isolated in 1915. • Now commercially grown, dried, compressed, & packaged. • Starts with whole, heated milk • Acids or enzymes (from bacteria) cause casein (milk protein) to denature & form curds. • Liquid part - whey. • Curds skimmed, dried, pressed, salted, & aged. • Sometimes innoculated with mold to enhance flavor. Cheesemaking Some Other Fermented Foods • • • • • Yogurt Wine vinegar Soy sauce Fermented rice Pickles • Both Beer making and bread making began in Egypt 6,000-5,000 BC • Beer- & bread makers often located next door & shared starters • No one knew microbes responsible for fermentation until Pasteur (late 1800’s) • Malt (germinated barley) & hops made into dough & fermented in water. Fermented Beverages Wine • Probably originated w/ accidental yeast contamination of grapes or juice Vinegar • Vinegar results from oxidation of wine or other fermented fruit juices, such as cider, by acetic acid bacteria.