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SOP for MPP+ (1-methyl-4-phenylpyridinium) # Chemical #2 Personal Protective Equipment (PPE) Standard Operating Procedures for Chemicals or Processes MPP+ is a hazardous chemical that is a metabolite of the neurotoxin MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine). Unlike MPTP, MPP+ is ionized and does not readily pass the blood-brain barrier. One use of MPP+ is to study Parkinson’s Disease if directly injected into the brain. Also, it may be used a surrogate for other more hazardous salts. When administered to mice, the compound is mostly excreted and minimally metabolized. The most likely route of exposure is via inhalation of the dry powder. - Laboratory coat, rear closing gown, or apron - Nitrile or other chemically resistant gloves - Goggles #4 Environmental / Ventilation Controls #5 Special Handling Procedures & Storage Requirements #6 Spill and Accident Procedures Dry MPP+ must be worked with in a chemical fume hood over plastic backed pads. When reconstituting a dry powder, weigh in the fume hood or tare weight the container and add the powder in the fume hood. Dilution should be done in a chemical fume hood. Reconstituted solutions may be handled outside of the fume hood. If using with other hazardous material (e.g., radiological or biological), follow containment requirements when working with these hazards. MPP+ should be stored in a cool, dry place. Follow specific instructions in the MSDS. Ensure that MPP+ is recorded in your online chemical inventory, MyChem. When working with the reconstituted liquid, work over plastic backed pads. MPP+ is more stable and is not easily inactive with chemicals, including bleach and potassium permanganate. At the conclusion of routine work, clean surfaces with 10% bleach for 30 minutes. Provide First Aid Immediately Sharps injury (needlestick and subcutaneous): Scrub exposed area thoroughly for 15 minutes using warm water and sudsing soap. Skin exposure: Use the nearest safety shower for 15 minutes. Stay under the shower and remove clothing. Use a clean lab coat or spare clothing for coverup. Eye exposure: Use the eye wash for 15 minutes while holding eyelids open. Inhalation: Move out of contaminated area. Get medical help. Contact the Employee Health Center immediately at (206)685-1026. If after hours, go immediately to the closest emergency room. For all other exposure, follow instruction outlined on the EH&S Exposure Poster: http://www.ehs.washington.edu/manuals/posters/ehsexposureresponseposter2 -9-11.pdf For powder spills in the fume hood, collected in a container for EH&S waste pick-up. Dispose of plastic backed pad as hazardous waste. Clean up the spill surface with 10% bleach. For powder spills outside the fume hood, evacuate the room. Remove and dispose of any contaminated PPE. Allow the dust to settle for 30 minutes. To clean-up the spill, pour 10% bleach so that it flows into the spill. Allow it to sit for 30 minutes. Clean up the spill with paper towel; dispose of the paper towels as hazardous waste. Clean the spill area again with 10% bleach. For liquid spills inside or outside the fume hood, wipe up the liquid with paper towels. Dispose of paper towels as hazardous waste. Clean the surface with 10% bleach for 30 minutes. #7 Waste Disposal #8 Special Precautions for Animal Use (if applicable) Unused concentrated MPP+ or similar liquid waste is managed as hazardous waste through EH&S. See http://www.ehs.washington.edu/epowaste/chemwaste.shtm Contaminated solids (including gowns and gloves believed to not have contact with MPP+) can be double bagged and discarded with regular garbage. When working with animals, follow safe sharps handling and disposal Sharps containers must be in close proximity to where sharps are being used. No re-capping, shearing or bending of needles. Used sharps must be disposed of as soon as possible. Re-usable sharps must be managed in such a manner to prevent accidental injury. If razor blades are re-used, they must be secured in a highly visible area. Change sharps containers promptly when the fill line is reached. Particularly hazardous X YES: Blocks #9 to #11 are Mandatory substance involved? NO: Blocks #9 to #11 are Optional. #9 Approval Training on the SOP and MPP+ MSDS must be done by the PI to all staff Required working with MPP+. EH&S Managing Laboratory Chemicals is required training and the online fume hood training is recommended. More information may be found at the EH&S website: http://www.ehs.washington.edu/psotrain/corsdesc.shtm #10 Decontamination After any work, decontaminate surfaces with 10% bleach for 30 minutes. Plastic backed pads and used PPE should be disposed of as garbage. #11 Designated Area The fume hood where the MPP+ will be handled must have current certification. Name: Signature: Environmental Health and Safety, Box 354400 Supervisor Title: Date: *to be filled in by PI or