Download SOP for MPP+ - Environmental Health and Safety

SOP for MPP+ (1-methyl-4-phenylpyridinium)
# Chemical
#2 Personal
Protective
Equipment (PPE)
Standard Operating Procedures for Chemicals or Processes
MPP+ is a hazardous chemical that is a metabolite of the neurotoxin MPTP
(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine). Unlike MPTP, MPP+ is
ionized and does not readily pass the blood-brain barrier. One use of MPP+
is to study Parkinson’s Disease if directly injected into the brain. Also, it
may be used a surrogate for other more hazardous salts. When administered
to mice, the compound is mostly excreted and minimally metabolized. The
most likely route of exposure is via inhalation of the dry powder.
- Laboratory coat, rear closing gown, or apron
- Nitrile or other chemically resistant gloves
- Goggles
#4 Environmental /
Ventilation Controls
#5 Special Handling
Procedures & Storage
Requirements
#6 Spill and Accident
Procedures
Dry MPP+ must be worked with in a chemical fume hood over plastic backed
pads. When reconstituting a dry powder, weigh in the fume hood or tare
weight the container and add the powder in the fume hood. Dilution should
be done in a chemical fume hood. Reconstituted solutions may be handled
outside of the fume hood. If using with other hazardous material (e.g.,
radiological or biological), follow containment requirements when working
with these hazards.
MPP+ should be stored in a cool, dry place. Follow specific instructions in
the MSDS. Ensure that MPP+ is recorded in your online chemical inventory,
MyChem.
When working with the reconstituted liquid, work over plastic backed pads.
MPP+ is more stable and is not easily inactive with chemicals, including
bleach and potassium permanganate. At the conclusion of routine work,
clean surfaces with 10% bleach for 30 minutes.
Provide First Aid Immediately
Sharps injury (needlestick and subcutaneous): Scrub exposed area
thoroughly for 15 minutes using warm water and sudsing soap.
Skin exposure: Use the nearest safety shower for 15 minutes. Stay under the
shower and remove clothing. Use a clean lab coat or spare clothing for coverup.
Eye exposure: Use the eye wash for 15 minutes while holding eyelids open.
Inhalation: Move out of contaminated area. Get medical help.
Contact the Employee Health Center immediately at (206)685-1026. If after
hours, go immediately to the closest emergency room. For all other exposure,
follow instruction outlined on the EH&S Exposure Poster:
http://www.ehs.washington.edu/manuals/posters/ehsexposureresponseposter2
-9-11.pdf
For powder spills in the fume hood, collected in a container for EH&S
waste pick-up. Dispose of plastic backed pad as hazardous waste. Clean up
the spill surface with 10% bleach.
For powder spills outside the fume hood, evacuate the room. Remove and
dispose of any contaminated PPE. Allow the dust to settle for 30 minutes.
To clean-up the spill, pour 10% bleach so that it flows into the spill. Allow it
to sit for 30 minutes. Clean up the spill with paper towel; dispose of the
paper towels as hazardous waste. Clean the spill area again with 10% bleach.
For liquid spills inside or outside the fume hood, wipe up the liquid with
paper towels. Dispose of paper towels as hazardous waste. Clean the surface
with 10% bleach for 30 minutes.
#7 Waste Disposal
#8 Special
Precautions
for Animal Use
(if applicable)
Unused concentrated MPP+ or similar liquid waste is managed as hazardous
waste through EH&S. See
http://www.ehs.washington.edu/epowaste/chemwaste.shtm
Contaminated solids (including gowns and gloves believed to not have
contact with MPP+) can be double bagged and discarded with regular
garbage.
When working with animals, follow safe sharps handling and disposal
 Sharps containers must be in close proximity to where sharps
are being used.
 No re-capping, shearing or bending of needles.
 Used sharps must be disposed of as soon as possible.
 Re-usable sharps must be managed in such a manner to prevent
accidental injury.
 If razor blades are re-used, they must be secured in a highly
visible area.
 Change sharps containers promptly when the fill line is reached.
Particularly hazardous
X YES:
Blocks #9 to #11 are Mandatory
substance involved?
NO:
Blocks #9 to #11 are Optional.
#9 Approval
Training on the SOP and MPP+ MSDS must be done by the PI to all staff
Required
working with MPP+. EH&S Managing Laboratory Chemicals is required
training and the online fume hood training is recommended. More
information may be found at the EH&S website:
http://www.ehs.washington.edu/psotrain/corsdesc.shtm
#10 Decontamination
After any work, decontaminate surfaces with 10% bleach for 30 minutes.
Plastic backed pads and used PPE should be disposed of as garbage.
#11 Designated Area
The fume hood where the MPP+ will be handled must have current
certification.
Name:
Signature:
Environmental Health and Safety, Box 354400
Supervisor
Title:
Date:
*to be filled in by PI or