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UCSF Lab Standard Operating Procedure Chemical Process, Name or Hazard Class: Diazomethane Please fill out the form completely. Insure all users have access to this SOP. Refer to instructions for assistance. Enter text. Department: Date SOP was written: Enter date. This lab specific SOP has been reviewed and approved by: Principal Investigator Name: Enter text. Principal Investigator Signature Lab Manager/Supervisor: Type of SOP: Process Enter text. Hazardous Chemical Hazardous Class Purpose: Diazomethane is extremely explosive at room temperature in its pure form. For safety and convenience diazomethane is always prepared as needed as a solution in ether and used as such. It converts carboxylic acids into their methyl esters or into their homologues. Diazomethane is also frequently used as a carbene source and a popular methylating agent. Physical & Chemical Properties/Definition of Chemical Group CAS# 334-88-3 Class: Acute Toxin Molecular Formula: CH2N2 Form (Physical State): Yellow gas with a musty odor Boiling point: -23°C Melting point: -145°C Diazomethane 1 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Potential Hazards/Toxicity Extremely Flammable, Explosive, Harmful by Inhalation, Frostbite by Skin Contact, Possible Carcinogen Engineering Controls Work with this chemical in a certified ducted fume hood. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Personal Protective Equipment (PPE) Respirator Protection If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/) Hand protection Handle with cold-insulating gloves. Gloves should be inspected prior to use. Wash and dry hands after handling. Eye protection Face shield or safety goggles. Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH Skin and body protection Wear long pants, closed toed shoes, and a flame retardant lab coat. Hygiene measures Avoid inhalation and contact with skin. Wash hands before breaks and immediately after handling the product. First Aid Procedures If inhaled Move person into fresh air. If not breathing give artificial respiration Consult a physician. Diazomethane 2 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) In case of skin contact Frostbite can occur if chemical is in its liquid physical form. Carefully rinse with plenty of water and do not remove clothes. Refer for medical attention. Wear protective gloves when administering first aid. In case of eye contact Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. Special Handling and Storage Requirements Precautions for safe handling Handle chemical in a well-ventilated area, away from shock, friction, and open flames. Prevent electric static build-up with a grounding cable. Conditions for safe storage Diazomethane should be stored in a cool, dry, well-ventilated area in tightly sealed containers. Spill and Accident Procedure Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.). Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up. Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance. Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends. Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct Diazomethane 3 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends. Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone) Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours. Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays). Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours. Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC). Decontamination/Waste Disposal Procedure Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste. Safety Data Sheet (SDS) Location Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html Protocol/Procedure Quantities covered by this SOP: ______ (g , ml) to _______ (g, ml) Temperature range covered by this SOP: __ °C – __ °C General Overview and Purpose: Enter the experimental purpose Procedure: Enter experimental procedure. You can copy procedure from your lab notebook or from literature. NOTE Any deviation from this SOP requires approval from the Principal Investigator. Diazomethane 4 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)