Download Diazomethane CAS No.334-88-3

UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
Diazomethane
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Hazardous Chemical
Hazardous Class
Purpose: Diazomethane is extremely explosive at room temperature in its pure form. For safety and
convenience diazomethane is always prepared as needed as a solution in ether and used as such. It converts
carboxylic acids into their methyl esters or into their homologues. Diazomethane is also frequently used as a
carbene source and a popular methylating agent.
Physical & Chemical Properties/Definition of Chemical Group
CAS#
334-88-3
Class:
Acute Toxin
Molecular Formula:
CH2N2
Form (Physical State):
Yellow gas with a musty odor
Boiling point:
-23°C
Melting point:
-145°C
Diazomethane
1
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Potential Hazards/Toxicity
Extremely Flammable, Explosive, Harmful by Inhalation, Frostbite by Skin Contact, Possible Carcinogen
Engineering Controls
Work with this chemical in a certified ducted fume hood. Facilities storing or utilizing this material should be
equipped with an eyewash facility and a safety shower.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand protection
Handle with cold-insulating gloves. Gloves should be inspected prior to use. Wash and dry hands after
handling.
Eye protection
Face shield or safety goggles. Use equipment for eye protection tested and approved under appropriate
government standards such as NIOSH
Skin and body protection
Wear long pants, closed toed shoes, and a flame retardant lab coat.
Hygiene measures
Avoid inhalation and contact with skin. Wash hands before breaks and immediately after handling the
product.
First Aid Procedures
If inhaled
Move person into fresh air. If not breathing give artificial respiration Consult a physician.
Diazomethane
2
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
In case of skin contact
Frostbite can occur if chemical is in its liquid physical form. Carefully rinse with plenty of water and do not
remove clothes. Refer for medical attention. Wear protective gloves when administering first aid.
In case of eye contact
Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
Special Handling and Storage Requirements
Precautions for safe handling
Handle chemical in a well-ventilated area, away from shock, friction, and open flames. Prevent electric static
build-up with a grounding cable.
Conditions for safe storage
Diazomethane should be stored in a cool, dry, well-ventilated area in tightly sealed containers.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
Diazomethane
3
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
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Procedure:
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NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
Diazomethane
4
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)