Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Common cold wikipedia , lookup
Gastroenteritis wikipedia , lookup
Urinary tract infection wikipedia , lookup
Hygiene hypothesis wikipedia , lookup
Neonatal infection wikipedia , lookup
Traveler's diarrhea wikipedia , lookup
Henipavirus wikipedia , lookup
Transmission (medicine) wikipedia , lookup
Hospital-acquired infection wikipedia , lookup
Cysticercosis wikipedia , lookup
Sociality and disease transmission wikipedia , lookup
Risk factors for a Clostridium difficile colonization in humans. Date: Student: Supervisor: Education: Course: 13-5-2013 M. van Schaik (3185338) E.C. Keessen (IRAS-VPH) Veterinary medicine, University of Utrecht research internship Abstract Clostridium difficile infection (CDI) can occur in a large number of animal species and in humans. An increase in incidence and severity of CDI in humans is reported since the beginning of this century. Symptomless colonization with the bacterium has been described in many animal species, including production animals. This could lead to an increased risk of infection of humans through direct contact with these animals or through indirect contact via the environment. Consumption of contaminated food products could also increase the risk of infection. The finding of overlapping C. difficile ribotypes in humans, animals and food products has led to the hypothesis that transmission of C. difficile via animals to humans contributes to the increasing number of patients with CDI. This study tries to determine the risk factors that lead to a colonization of C. difficile in humans working with pigs or live at a pig farm. To analyze the data a multivariate logistic regression model is used. The software program for the multivariate logistic regression model is SAS 9.2 (SAS institute Inc., Cary, North Carolina, USA). No significant correlation is found between the risk factors and colonization of C. difficile in human using the multivariate logistic regression model with backward elimination. An univariate model is used to select variables for the multivariate logistic regression model. In the univariate model two significant risk factors are determined. One risk factor is a high contact frequency between persons and pigs (p=0.03). The other risk factor is administration of antibiotics to the pigs (p=0.06). Introduction C. difficile is a gram positive anaerobe bacterium. C. difficile produces endospores and toxins. C. difficile infection (CDI) is a growing problem in hospitals in Europe and northern America. The incidence of outbreaks of C. difficile ribotype 027 is rising In Dutch hospitals (3,4). The incidence of infection with C. difficile ribotype 078 rises in hospitals in the Netherlands (15) and in Europe(16). On the basis of geno- and phenotypical analyses it is concluded that strains C. difficile ribotype 078 from piglets were indistinguishable from human strains in the Dutch population. A common origin of human and animal strains of C. difficile should be considered (3). The results of multiple studies suggest that animal reservoirs are possible sources, via food, direct contact, or via the environment of human C. difficile infection (2,8). However there are still many uncertainties about the connection of the specific C. difficile ribotypes in humans and animals. CDI has emerged as a significant economic concern for swine producers, but little is known about transmission patterns in hog operations (5, 6). In neonatal piglets, C. difficile is currently one of the major causes of enteritis (11). In this study the risk factors at pig farms that lead to colonization with C. difficille in humans are determined. Some case control studies about risk factors for CDI are done but the risk factors in these studies concern the use of medicine in human patients and the intervals of visiting healthcare institutions. The questions in the questionnaire used for this study are related to animal contact, use of antibiotics, the management at the pig farm and the working procedures, with a focus on the hygienic measures and contact frequencies between pigs and humans. The risk factors that are selected in this study are derived from other studies with different pathogens, like MRSA and based on epidemiological knowledge. Multiple studies suggest that antibiotic treatment is a main risk factor for developing CDI in humans (9) and in horses (10), that is the reason why antibiotic treatment is a risk factor that is investigated in this study. The protocol of the study was approved by the Medical Ethical Committee of Utrecht University. Methods and Material The colonization with C. difficile was investigated by taking human stool samples of 138 participants. The participants all work or live at a pig farm in the Netherlands. 40 farms joined the Bactopath project. The human stool samples are collected by the person itself, the stool sample is put in a plastic air tight container and in an airtight bag. The bag is send by normal mail to the laboratory. At arrival the samples were frozen to -20°C. All humans who work or live on pig farms of which samples are collected completed a personal questionnaire about possible risk factors. This includes the work method on the pig farm, the method of cleaning, (personal) hygienic methods and procedures, and the way the farm is managed. Other questions were related to whether the person administered antibiotics to the pigs and concerned the contact frequency between the person and the pigs. C. difficile detection is performed using standard protocols for samples from human and animal origin as described by Hopman et al. (2011). One sample of each person is used. The samples are defrosted once. In short, 1 gram of faeces is mixed with 9 ml of the CDMN broth and incubated anaerobe at 37°C for 7 days. The broth is alcohol-shocked for spore selection by mixing 2 ml homogenized broth with 96% ethanol in a sterile tube and leaving it for 1 hour at room temperature. After centrifuging this mixture (4000 x g for 10 minutes) the supernatant is discarded, and the pellet is streaked onto a CLO agar plate and incubated anaerobe for 48 hours at 37°C. Suspicious colonies based on gram stain appearance, colony morphology (swarming, non-haemolytic, greyish, rough) and characteristic odour are sent to the Leiden University Medical Center (LUMC) for PCR ribotyping. In some cases Molecular characterization of selected type 078 and 045 isolates is performed to determine genetic relatedness of the isolates. Data analysis A multivariate logistic regression model with backward elimination is performed to investigate the risk factors. Criteria for variables to be included in multivariate analysis are that no more than 10% of the data is missing in relation with the outcome. The next step is to perform an univariate statistical test using the chi² method. For this test SPSS 20 (IBM Corporation Software Group, Somers, NY, USA)software program is used. All results with a p-value < 0.3 are considered for the multivariate model. In the multivariate model P-values <0.05 are considered statistically significant. To perform the logistic regression model the software program SAS 9.2 (SAS institute Inc., Cary, North Carolina, USA) is used. Results 138 persons participated in this study. C. difficile was found in fecal samples of 13 farmers and employees, 5 partners, and in none of the children. Only participants who reported to have direct contact with pigs were colonized with the bacterium, however this was not a significant risk factor (p=0.072). The following variables meet the criteria (p-value < 0.3 ) to be used in the multivariate logistic regression model. Weekly working hours; residence on the pig farm; relocate sows; relocate piglets; relocate weaning pigs; feeding sows; feeding piglets; feeding weaning pigs; cleaning sows; performing healthcare to piglets; contact with fattening pigs; cleaning departments of piglets; use of a facemask; contact with pigs on other farms; contact with own pigs; presence in the departments of own pigs; presence in the departments of pigs on other farms; presence in stables with sheep; administration of antibiotics; cleaning hands before entering pig farm; cleaning hands after entering pig farm; and frequency of contact with own pigs. Univariate results Two risk factors are statistical significant in the univariate analysis. Contact frequency between persons and pigs (p=0.03) and the administration of antibiotics to the pigs (p=0.006). The contact frequency is divided into two variables, contact on a daily/weekly base versus contact on a monthly/yearly base. The administration of antibiotics is divided into two groups. A group who administrated antibiotics to pigs and a group who didn’t. Multivariate results Due to redundancy all effects were removed from the multivariable logistic regression model and no variables in the model are available for display on the X-axis. This means there is no significant risk factor determined in this model. Discussion Due to the fact that there are no previous risk factors documented a number of possible risk factors is selected in order to make this study possible. The selection was made on the base of risk factors described in previous studies about CDI and the knowledge gained from documented risk factors from other bacteria then C. difficile. It is likely that risk factors concerning C. difficile are similar as to other bacteria with the same characteristics. Two results suggest that the contact frequencies between humans and pigs are an important risk factor of C.difficile colonization in humans. The first is that only participants who reported to have direct contact with the pigs were colonized with the bacterium, however this was not a significant risk factor (p=0.072). The second is the contact frequency between persons and pigs (p=0.03). Both results are expected because the transmission of C. difficile between animals and humans can occur due to direct contact. The data used to measure the influence of antibiotic treatment in this model is derived from the questionnaire. The question referred to whether participants personally administrated antibiotics to the animals or not. A significant result was found in the univariate model (p=0.006). Antibiotics are a known risk factor for developing CDI in humans (9). A possible explanation is the direct contact between pigs and the administrator. Another explanation is the direct contact between the antibiotics and the skin of the administrator during administration. Because of this contact there is a disturbance in the micro flora of the administrator that gives C. difficile an opportunity to colonize. In a parallel study in the Bactopath project, quantitative data is available about how much antibiotics a farm uses and what sort of antibiotics they use. In a follow up study it would be interesting to use this data in combination with the colonization of C. difficile in humans. Questionnaires always carry the risk of bias. The questionnaire was intensive, that is why it is possible that people did not fill out the questions as precise as they could due to lack of time or loss of interest. In further studies it would be interesting to focus on the contact structures between humans and pigs, how intense is the contact and how do specific methods of handling pigs differ between humans who work with pigs. In further studies a different study design would be recommended. For example follow participants that are not yet colonized with C. difficile for a period of time. Divide the participants in two groups. One group has frequent contact with pigs or administer antibiotics to the pigs. The other groep does not. One downside to our methodology is that the authors had no previous experience with the softwareprogram SAS. Conclusion None of the risk factors are significant in a logistic regression statistical analysis with backward elimination. However the risk factors contact frequency and antibiotic administration give a significant outcome in a univariate analysis. Only participants who reported to have direct contact with the pigs were colonized with the bacterium, however this was not a significant risk factor. The conclusion in this study is that none of the risk factors have had a significant role in the prevalence of C. difficile in humans. Further research is necessary to explain the role of the univariate results that have a significant outcome. This research should focus on the contact structures between humans and pigs, and on quantitative data concerning antibiotic administration to pigs. Literature 1. Keel, K., Brazier, J. S., Post, K. W., Weese, S. & Songer, J. G. Prevalence of PCR ribotypes among Clostridium difficile isolates from pigs, calves, and other species. J. Clin. Microbiol. 45, 1963-1964 (2007). 2. Indra A., Lassnig H., Baliko N., et al. Clostridium difficile: a new zoonotic agent? Wiener klinischer Wochenschrift( 2009) 121:91-95 3. Kuijper, E.J., Van den Berg, R.J., Debast, S., Visser, C.E., Veenendaal, D., Troelstra, A., et al. (2006b) Clostridium difficile PCR ribotype 027, toxinotype III in The Netherlands. Emerg Infect Dis 12: 827–830. 4. Goorhuis, A. et al. Spread and epidemiology of Clostridium difficile polymerase chain reaction ribotype 027/toxinotype III in The Netherlands. Clin. Infect. Dis. 45, 695-703 (2007). 5. Songer, J., K. Post, D. Larson, B. Jost, and R. Glock. 2000. Infection of neonatal swine with Clostridium difficile. Swine Health Prod. 8:185–189. 6.Waters, E. H., J. P. Orr, E. G. Clark, and C. M. Schaufele. 1998. Typhlocolitis caused by Clostridium difficile in suckling piglets. J. Vet. Diagn. Investig. 10:104–108. 7. Keessen ,E.C., W. Gaastra,, and L.J.A. Lipman. 2010. Clostridium difficile infection in humans and animals, differences and similarities. Veterinary Microbiology VETMIC-5243 8. Marwah M. Bakri, Derek J. Brown, John P. Butcher, and Alistair D. Sutherland, 2009, Clostridium difficile in Ready-to-Eat salads, Scotland Emerging Infectious Diseases, Vol. 15, No. 5, May 2009 9. Kuijper, E.J., Van Disselt, J.T. 2008.Spectrum of Clostridium difficile infections outside health care facilities. Canadian Medical Association journal, journal de l'Association medicale canadienne 179, 747-748. 10.Madewell, B.R., Tang, Y.J., 1995.Apparent outbreaks of Clostridium difficile-associated diarrhea in horses in a veterinary medical teaching hospital. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 7, 343346. 11. Songer, J.G., Anderson, M.A. (2006). Clostridium difficile: an important pathogen of food animals. Anaerobe 12, 1-4. 12. Baverud, V., Gustafson, A. 1997. Clostridium difficile associated with acute colitis in mature horses treated with antibiotics. Equine veterinary journal 29, 279-284. 13.Ruby, R., Magdesian, K.G., Kass, P.H. 2009. Comparison of clinical, microbiologic, and clinicopathologic findings in horses positive and negative for Clostridium difficile infection. Journal of the American Veterinary Medical Association 234, 777-784 14. Lawley, T.D., Clare, S., 2009. Antibiotic treatment of clostridium difficile carrier mice triggers a supershedder state, spore-mediated transmission, and severe disease in immunocompromised hosts. Infection and immunity 77, 3661-3669. 15. Kuijper, E.J., Hensgens, M.P.M. Clostridium difficile infection in an endemic setting in the Netherlands. European journal of clinical microbiology and infectious diseases, Volume: 30 Issue: 4 (April 1, 2011), pp: 587-593 16. Dubberke, E.R., Hoppe-Bauer, J. Impact of Clinical Symptoms on Interpretation of Diagnostic Assays for Clostridium difficile Infections. Journal of clinical microbiology, Volume: 49 Issue: 8 (August 1, 2011), pp: 2887-2893