Download Engineering of E.coli for production of poly(3

Engineering of E.coli for production
of poly(3-hydroxypropionate)
Tallyne Vasconcelos
What are polyhydroxyalkanoates (PHAs)?
 biodegradable and biocompatible thermoplastics used in several
applications, such as medicine, pharmacy and agriculture
 the production was a way of using the glycerol in excess originated from
the biodiesel industry
 poly(3-hydroxypropionate) was the type of PHA used in this experiment
Why to use E.coli?
 E.coli strains are widely used as hosts for microbial production of valuable
compounds, like biofuels, chemicals and polymers
 It is highly adaptable and widely know genetic and metabolically
The process
Results
Results
Conclusion
 the microbial P3HP production from glycerol was improved greatly by
constructing a genetically stable E.coli recombinant strain
 To overcome the instability due to plasmid loss, two strategies were tested
and combined
 Better results were obtained: the recombinant strain Q1738 produced 25.7
g/L of P3HP from glycerol in aerobic fermentation
Thank you!