Download Genetic Diversity of ploidy level Miscanthus species in Japan

Genetic Diversity of ploidy level Miscanthus species in
Japan
Marhamah Nadir1 and Aya Nishiwaki2
Dept. of Animal Feed and Forages, Animal Science Faculty, University of Hasanuddin
2 Field Science Centre, Faculty of Agriculture, University of Miyazaki
Corresponding author: [email protected]
Abstract
Miscanthus species are of potential value as bio-resources. Among the available
species, Giant Miscanthus (Miscanthus × giganteus), a highly productive sterile
triploid hybrid grass that was discovered in Japan several decades ago, has
considerable potential as an alternative source of energy. The aim of this study is to
measure DNA content variation of each species using flow cytometry which is more
beneficial and efficient to count large scale genetic diversity in ploidy level and
genome size variation to study genetic resource of Miscanthus species. Samples
collected from 24 localities in Japan and contains of eight species of Miscanthus like
M. intermedius, M. tinctorius, M. oligotachyus, M. florindulus, M. sinensis var
condensatus, M. sinensis, M. sacchariflorus and M. xgiganteus. All samples were
determined DNA content (pg.2C-1) using flowcytometry. The mean DNA contents of
Miscanthus species were 16.53 pg M. intermedius, 5.80 pg M. oligostachyus, 5.69 pg
M. tinctorius, 5.19 pg M. floridulus and 5.38 pg M. sinensis var. condensatus. It is clear
that M. intermedius is a 6x species. I also discovered a 4x M. intermedius plant. There
were clearly different DNA contents between 4x M. intermedius and 6x M.
intermedius.
Keywords : Miscanthus, bioenergy, flow cytometry, M. intermedius, hybrids
Field of presentation: Cellular Science
Type of presentation: Oral
Genetic Diversity of ploidy level Miscanthus species in Japan
Marhamah Nadir1 and Aya Nishiwaki2
1
Dept. of Animal Feed and Forages, Animal Science Faculty, University of Hasanuddin
2
Field Science Centre, Faculty of Agriculture, University of Miyazaki
Miscanthus species are of potential value as bio-resources. Among the available species,
Giant Miscanthus (Miscanthus × giganteus), a highly productive sterile triploid hybrid
grass that was discovered in Japan several decades ago, has considerable potential as an
alternative source of energy. The aim of this study is to measure DNA content variation
of each species using flow cytometry which is more beneficial and efficient to count large
scale genetic diversity in ploidy level and genome size variation to study genetic resource
of Miscanthus species. Samples collected from 24 localities in Japan and contains of
eight species of Miscanthus like M. intermedius, M. tinctorius, M. oligotachyus, M.
florindulus, M. sinensis var condensatus, M. sinensis, M. sacchariflorus and M.
xgiganteus. All samples were determined DNA content (pg.2C-1) using flowcytometry.The
mean DNA contents of Miscanthus species were 16.53 pg M. intermedius, 5.80 pg M.
oligostachyus, 5.69 pg M. tinctorius, 5.19 pg M. floridulus and 5.38 pg M. sinensis var.
condensatus. It is clear that M. intermedius is a 6x species. I also discovered a 4x M.
intermedius plant. There were clearly different DNA contents between 4x M. intermedius
and 6x M. intermedius.