Download Optimization of Glucose Production from Oil Palm Trunk through

International Conference on Biotechnology, Nanotechnology and its applications (ICBNA'2012) March 17-18, 2012 Bangkok
Optimization of Glucose Production from Oil
Palm Trunk through Acid Hydrolysis
Shervan Babamohammadi1, Ida Idayu Muhamad2
Abstract— There are large amount of biomass residue in
Malaysia that one of the most abundant one is Oil Palm (Elaeis
guineenis) Trunk (OPT). It contains cellulose, hemicellulose and
lignin of almost 41.2%, 34.1% and 24.7% respectively, which lignin
can be removed by pretreatment and then cellulose and hemicellulose
can be converted to reducing sugar by hydrolysis. Subsequently, by
fermentation process, glucose converts to ethanol or lactic acid by
using proper micro-organism. In this study, producing glucose from
OPT through acid hydrolysis was investigated. Respond Surface
Methodology (RSM) was used to evaluate the interactive effect of
acid concentration (1-3%) and the weight ratio of OPT to acid
solution in hydrolysis reaction (5-15% w/v) and type of acid in three
categories (A: pretreated with NaOH and treated with H 2 SO 4 , B:
pretreated with NaOH and treated with HCl and C: only treated with
H 2 SO 4 ) on the yield of glucose. Therefore it would be obtained the
optimum acid concentration and weight ratio in the most proper type
of acid hydrolysis. It is also clarified the effect of NaOH pretreatment
on glucose yield. RSM has been carried out by Design Expert 6.0.4
and optimum conditions were derived: pretreated with NaOH and
hydrolysis by using 1.84% H 2 SO 4 when the weight ratio of OPT to
sulfuric acid solution is 5.59% (w/v). In these conditions the
experimental yield of glucose is 38.07% while the predicted value is
38.44% which shows the model is sufficiently desirable
.
Keywords— Acid hydrolysis, Glucose, Oil Palm Trunk, RSM.
1: Shervan Babamohammadi is with Chemical Engineering Department ,
Faculty of Chemical Engineering Universiti Teknologi Malaysia, 81310
Skudai, Johor, Malaysia (corresponding author to provide phone:
+60177217792; e-mail: [email protected]).
2: Ida Idayu Muhamad is with Bioprocess Engineering Department ,
Faculty of Chemical Engineering Universiti Teknologi Malaysia, 81310
Skudai, Johor, Malaysia (e-mail: [email protected]).
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