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DOC/LP/01/28.02.02
LP- BT 2203
LESSON PLAN
LP Rev. No: 01
Sub Code & Name:
Date: 28.06.12
BT 2203
Page 1 of 6
BIOCHEMISTRY I
Unit: I
UNIT: I
Branch: Biotechnology
Semester: III
: INTRODUCTION TO BIOMOLECULES
5
Basic principles of organic chemistry, types of functional groups, biomolecules, chemical
nature, water, pH and biological buffers.
OBJECTIVE: To introduce the Basic concepts and Biomolecules in Biochemistry
Session
No
Time
(min.)
Topics to be covered
Text Book/
Ref Book
Page
No
Teaching
Method
1
Introduction to Biochemistry
50
Ref I
1-13
BB/OHP
2
Introduction to biomolecules
50
Ref I
1-13
BB/OHP
3
Basic principles of Organic chemistry
50
1-13
BB/OHP
4
Types of functional groups
50
Ref 1
13-14
SBC
5
Biomolecules
50
Ref I
45-60
SBC
6
Chemical nature of Water and its importance in
Biochemistry
50
Ref I
45-60
BB/OHP
7
pH and Biological buffers
50
Ref I
61-70
BB/OHP
8
pH calculation
50
Ref I
61-70
BB
1
Ref I
LESSON PLAN
DOC/LP/01/28.02.02
LP- BT 2203
LP Rev. No: 01
Date: 28.06.12
Sub Code & Name: BT 2203 BIOCHEMISTRY I
Unit: II
Branch: Biotechnology
Semester: III
Page 2 of 6
UNIT II STRUCTURE AND PROPERTIES OF IMPORTANT BIOMOLECULES (15)
Carbohydrates:
(Mono,
di,
oligo
&
polysaccharides)
Proteoglycans,
glucosaminoglycans. mutarotation, glycosidic bond, reactions of monosaccharides,
reducing sugars .
Lipids: fatty acids, glycerol, saponification, iodination, hydrogenation, phospholipids,
glycolipids, sphingolipids, cholesterol, steroids, prostaglandins.
Proteins: Amino Acids, Peptides, Proteins, measurement, structures, hierarchy of
organization primary, secondary, tertiary and quaternary structures, glycoproteins,
lipoproteins. Determine of primary structure.
Nucleic acids: purines, pyrimidines, nucleoside, nucleotide, RNA, DNA, reactions,
properties, measurement, nucleoprotein complexes
OBJECTIVE: To discuss in detail the structures and properties of carbohydrates,
lipids, proteins and Nucleic acids
Session
No
9
10
11
12
13
14
Topics
to
be Time
covered
(min)
Mono, di, oligo &
50
polysaccharides
Proteoglycans,
50
glucosaminoglycans.
Mutarotation,
50
glycosidic bond
Reactions
of
monosaccharides,
50
reducing sugars
Fatty acids and
glycerol
Saponification,
iodination,
hydrogenation,
Ref
Ref I
Ref I
Ref I
Ref I
Ref I
50
Ref I
50
Ref I
15
Phospholipids,
glycolipids
50
2
Page No
Page No
239 to 255
Page No
255 to 266
Page No
242 & 245
Page No
244 to 245
Page No
343 to 348
Internet
Sources
Page No
348 to 354
Teaching
Method
BB/OHP
SB
BB/OHP
BB/OHP
SB
BB/OHP
SB
16
Sphingolipids,
17
Cholesterol,
steroids,
prostaglandins
18
19
Peptides, Proteins
and Measurement
Hierarchy of
organization
primary, secondary,
tertiary and
quaternary structures
Ref I
50
Ref I
50
50
50
Ref I
Ref I
20
Glycoproteins,
Lipoproteins
25
Introduction to
proteins
Determination of
primary structure of
proteins
Purines, nucleoside
and nucleotide
Pyrimidines,
nucleoside and
nucleotide
RNA, Reactions
26
DNA, Reactions
27
Properties,
measurement
Nucleoprotein
complexes
21
22
23
24
28
50
Ref I
50
Ref I
50
Ref I
Ref I
50
Ref I
50
50
Ref I
50
Ref I
50
Ref I
50
Ref I
3
Page No
348 to 354
Page No
355 &
Page No
358 to 360
Page No 75
to 89
Page No 96
& Page
No
117 to 152
Page No
258 to 260
Page No
820-824
Page No 96
to 104
Page No 96
to 104
Page No
273 to 279
Page No
273 to 279
Page No
278-279
Page No
278-279
Page No
278-279
Page No
278-279
SB
BB/OHP
SB
BB/OHP
BB/OHP
SB
SB
BB/OHP
BB/OHP
BB/OHP
BB/OHP
BB/OHP
BB/OHP
DOC/LP/01/28.02.02
LP-BT2203
LESSON PLAN
LP Rev. No: 01
Date: 28.06.12
Sub Code & Name: BT 2203 BIOCHEMISTRY I
Unit: III
Branch: Biotechnology
Semester: III
Page 3 of 6
UNIT III: METABOLISM CONCEPTS
5
Functions of Proteins, Enzymes, introduction to biocatalysts, metabolic pathways,
primary and secondary metabolites
OBJECTIVE: To discuss in detail the structures and properties of lipids and nucleic
acids
Session
No
Topics to be covered
Time
(min)
Ref
29
Functions of Proteins
50
Ref I
30
Enzymes
50
Ref I
31
Introduction to biocatalysts
50
Ref I
32
Metabolic pathways
50
33
Primary metabolites
50
34
Secondary metabolites
50
35
Degradation pathway
50
36
Question bank discussion
4
Ref I
Ref I
Ref I
Ref I
Page No
Page No
157
Page No
190
Page No
191-233
Page No
489-494
Page No
507-515
Page No
507-515
Page No
507-515
Teaching
Method
SB
BB/OHP
BB/OHP
BB/OHP
BB/OHP
BB/OHP
BB/OHP
DOC/LP/01/28.02.02
LP-BT2203
LESSON PLAN
LP Rev. No: 01
Sub Code & Name: BT2203 BIOCHEMISTRY I
Unit: IV
UNIT IV
Branch: Biotechnology
Date: 28.06.12
Page 4 of 6
Semester: III
INTERMEDIARY METABOLISM AND REGULATION
15
Glycolysis, TCA cycle, gluconeogenesis, pentose phosphate shunt, glyoxalate shunt, fatty
acid synthesis and oxidation, reactions of amino acids, deamination, transamination and
decarboxylation, urea cycle, interconnection of pathways and metabolic regulation. Case
study on overproduction of glutamic acid, threonine , lysine, methionine, isoleucine and
ethanol.
OBJECTIVE: To impart knowledge of metabolism of biomolecules and its
regulation
Session
No
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
Topics to be covered
Glycolysis
TCA Cycle
Gluconeogenesis
Pentose phosphate shunt
Glyoxalate shunt
Fatty acid synthesis
Fatty acid oxidation
Reactions of amino acids, deamination,
transamination
Reactions of amino acids decarboxylation,
Urea cycle
Interconnection of pathways and metabolic
regulation
Case study on overproduction of glutamic
acid and threonine
Case study on overproduction of lysine
and methionine
Case study on overproduction of
isoleucine and ethanol
Urea cycle degradation
Deamination and transamination
Time
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
5
Ref
Ref I
Ref I
Ref I
Ref I
Ref I
Ref I
Ref I
Ref I
Ref I
Ref I
Ref I
Internet
Sources
Internet
Sources
Internet
Sources
Ref I
Ref I
Page No
522-534
606- 620
543-548
549-554
623--624
787-804
637-648
661
859
665
668
Internet
Sources
Internet
Sources
Internet
Sources
665
661
Teaching
Method
BB/OHP
BB/OHP
SBC
BB/OHP
BB/OHP
SBC
BB/OHP
BB/OHP
SBC
BB/OHP
BB/OHP
BB/OHP
SBC
BB/OHP
BB/OHP
BB/OHP
DOC/LP/01/28.02.02
LP-BT 2203
LESSON PLAN
LP Rev. No: 01
Date: 28.06.12
Sub Code & Name: BT2203 BIOCHEMISTRY I
Unit: V
UNIT V
Branch: Biotechnology
Semester : III
Page 5 of 6
BIOENERGETICS
5
High energy compounds, electronegative potential of compounds, respiratory chain, ATP
cycle, and calculation of ATP yield during oxidation of glucose and fatty acids.
OBJECTIVE: To impart knowledge of intermediary metabolism and bioenergetics.
Session
No
53
54
55
56
57
58
59
60
Topics to be covered
High energy compounds
Electronegative potential of
compounds
Respiratory chain
ATP cycle
Calculation of ATP yield
oxidation of glucose
oxidation of fatty acids
Transamination
6
Time
(min)
50
Ref
Page
No
Teaching
Method
Ref I
496-506
SB
50
Ref I
507-516
SB
50
50
50
50
50
50
Ref I
Ref I
Ref I
Ref I
Ref I
Ref I
734-745
740-744
650-652
650-652
650-652
661
SB
OHP
OHP
OHP
OHP
BB/OHP
DOC/LP/01/28.02.02
LP-BT 2203
LESSON PLAN
LP Rev. No: 01
Date: 28.06.12
Sub Code & Name: BT2203 BIOCHEMISTRY I
Branch: Biotechnology
Page 6 of 6
Semester : III
Course delivery plan:
WEEK
UNITS
1
2
3
4
I
5
6
II
7
8
9
10
11
III
12
13
IV
Text Books:
1. Lehninger’s Principles of Biochemistry by David L.Nelson and Michael M.Cox,
Macmillan Worth publisher.
2. Lubert Stryer, Biochemistry, 4th edition,W.H.Freeman and co.,2000
3. Murray, R.K., Granner, B.K., Mayles, P.A., Rodwell, V.W., Harper’s Biochemistry
Prentice Hall International.
4. Voet and Voet, Biochemistry 2nd Edition, John Wiley and Sons Inc., 1995
5. B.S.Bahl, Arun Bahl., Advanced organic Chemistry 1st Edition, S.Chand & Co.Ltd.,
2000
Prepared by
Approved by
Dr.N.Karpagam
Prof. Sulochana Somasundaram
Assistant Professor (BT)
HOD of Biotechnology
28.06.12
28.06.12
Signature
Name
Designation
Date
7
14
15
V
8