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Mitosis Microscope Activity Objectives: ● To observe the phases of mitosis in real cells. ● To notice differences between plant and animal cells. ● To determine how long cells stay in each phase of the cell cycle. Procedure: 1. Obtain a microscope and 2 prepared slides: ● whitefish blastula (animal cells) and onion root tip (plant cells) 2. First place the onion root tip slide on the stage of a microscope. 3. Look at the cells under low power (10x) focus on about 2050 cells. 4. Count the total number of cells in the field of view. Record that in the chart below. 5. Identify the number of cells in interphase, prophase, metaphase, anaphase, and telophase/cytokinesis. Record the numbers in the chart below. They should add up to the total number of cells on the slide. 6. Calculate the percent of time cells stay in each phase. 7. Graph the results by making a line graph below (connect the points). 7. Next, zoom in (40x) on a cell in each phase and draw them below. In ONE drawing, label the following: cell membrane, centriole, chromosome, nuclear membrane, and spindle. 8. Repeat step 7 with the whitefish blastula slide. Be sure to label ONE drawing. Stage of Cell Cycle Interphase Prophase Metaphase Anaphase Telophase/Cytokinesis Total Number of cells Percentage of Time (#cells/total cell count) Title:________________________________________________________________ Type of cell: ____________________________ Interphase: Prophase: Metaphase: Telophase: Anaphase: How does cytokinesis occur in this type of cell? Type of cell: ____________________________ Interphase: Prophase: Metaphase: Telophase: Anaphase: How does cytokinesis occur in this type of cell? Analysis Questions: 1. Which cell cycle phase takes the longest to complete? ______________________ 2. What were 2 differences you noticed between plant and animal cells? 3. What is happening inside all of those cells in interphase?