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Transporting Molecules Diffusion http://lhs.lps.org/staff/sputnam/Biology/U3Cell/diffusion_1.png Animatioin from: http://www.biologycorner.com/resources/diffusion-animated.gif Molecules move from “where there’s A LOT” to “where there’s NOT” DIFFUSION across a space Happens anytime there is a _____________ DIFFERENCE in concentration in one place compared to another = Concentration gradient (________________________) DIFFUSION across a space • Molecules move automatically from an area of _______ Higher concentration to an area of ________ Lower concentration • EX: _____________________ Perfume, Koolaid, popcorn, BBQ, Bad smell in room _____________________ http://leighhouse.typepad.com/blog/images/kool_aid.jpg http://www.swapmeetdave.com/Humor/Farts.htm Molecules need to move across membranes in cells Image modiified from: http://www.accessexcellence.org/AB/GG/importProt.html If there is a difference in concentration on two sides of a membrane… Diffusion can happen ACROSS A MEMBRANE too! …as long as membrane will let molecule through SELECTIVELY PERMEABLE Image modified from: http://www.medaille.edu/vmacer/120_graphic_05oxygen.gif EXAMPLE: Oxygen molecules move automatically from lungs into blood DIFFUSION moves them from HIGHER (lungs) concentration to LOWER concentration (blood) What if cell needs to move large or polar molecules? EX: Glucose Amino acids What if cell needs to move a molecule AGAINST the CONCENTRATION GRADIENT? (LOW HIGH) Example : Glucose into mitochondria Image from: http://www.biologyclass.net/mitochondria.jpg What if cell needs to move molecules really FAST? (can’t wait for it to diffuse) Example in cells: Movement of + + Na & K ions sends nerve signals http://www.steve.gb.com/images/science/neuron.png WAYS MOLECULES MOVE ACROSS MEMBRANES • PASSIVE TRANSPORT Does NOT require energy • ACTIVE TRANSPORT Requires energy Kidspiration by: Riedell Kinds of Passive Transport • Diffusion __________________________________ •Osmosis •Facilitated Diffusion ___________________________________ •Ion Channels ___________________________________ ___________________________________ DIFFUSION across a membrane • Moves from: HIGHER concentration LOWER Any kind of molecule that can pass through the membrane can do this. OXYGEN EX: ______________ CARBON DIOXIDE ______________ http://www.lionden.com/cell_animations.htm OSMOSIS • MOVES WATER MOLECULES • Across a SEMI-PERMEABLE MEMBRANE • Moves from HIGHER LOWER http://www.Isbu.ac.uk/water/molecule.html What if there is a difference in concentration but solute molecules can’t move across a membrane? WATER will move until concentration reaches equilibrium Osmosis1 http://www.sidwell.edu/us/science/vlb6/labs/a_osmosis.gif OSMOSIS Osmosis4 HYPERTONIC: Concentration outside cell is ____________________ GREATER THAN inside cell More water leaves cell than enters shrinks so cell ____________ OSMOSIS Osmosis3 HYPOTONIC: Concentration outside cell is ________________ LESS THAN inside the cell More water enters than leaves cell so cell will ___________________ Swell bigger OSMOSIS ISOTONIC: EQUALS Concentration outside cell ____________ concentration inside cell Water entering = water leaving STAYS THE SAME SIZE so cell _____________________ Animal cells = cytolysis = crenation http://www.stchs.org/science/courses/sbioa/metenergy/bloodcells.gif http://www.stchs.org/science/courses/sbioa/metenergy/aplantturgor.gif Plant cells No cytolysis/cell wall keeps it from bursting = Plasmolysis VACUOLES store WATER http://www.biology4kids.com/files/cell_vacuole.html TURGOR PRESSURE = _______________________ Pressure of water molecules pushing against cell wall SO WHAT? SO WHAT? Bath water is HYPOTONIC compared to you Sitting in the bathtub causes your fingers and toes to wrinkle up when water enters your skin cells by osmosis Kinds of Passive Transport • Diffusion __________________________________ •Osmosis •Facilitated Diffusion ___________________________________ •Ion Channels ___________________________________ ___________________________________ Facilitated Diffusion Carrier proteins help diffusion go faster FACILITATED DIFFUSION NO energy required • Passive = ____________________ HIGH LOW • Moves from ________________________ Carrier Proteins • _____________________ bind molecule, change shape, and release it on other side of membrane Integral (Carrier proteins are _______________ proteins) • Molecules that move this way in cells: GLUCOSE _______________________ Facilitated Diffusion Animation from: http://bio.winona.edu/berg/ANIMTNS/facdifan.gif Kinds of Passive Transport • Diffusion __________________________________ •Osmosis •Facilitated Diffusion ___________________________________ •Ion Channels ___________________________________ ___________________________________ ION CHANNEL ION CHANNELS PASSIVE • _____________transport (no energy required) HIGH LOW • Molecules move from ___________________ • Small passageways for _______ IONS to get through membrane • Each ion has own kind of passageway • Examples in cells: Na+ , Cl- , Ca + + , K + ION CHANNELS Some channels always open Some channels have “gates” that open or close in response to signals SIGNALS: •stretching of cell membrane •electrical signals •chemicals Animation from: http://www2.uic.edu/~myilma1/ionchannel.gif Kidspiration by: Riedell Kinds of Active Transport •________________________________________ Sodium-Potassium Pump •Endocytosis ___________________________________ •Exocytosis ____________________________________ SODIUM-POTASSIUM PUMP ATP • ___________ ACTIVE transport (requires energy from ______) • Special just for Na+ and K + ions Carrier Proteins to move molecules • Uses integral ___________________ • Examples in nerve cells: Na+ is pumped out of cells at same time K + is taken into cells + Na and K + Animation from: http://www.lionden.com/cell_animations.htm PUMP ENDOCYTOSIS Takes substances into cell • _____________transport (requires energy from ATP) ACTIVE • Uses small membrane sacs to carry substances = VESICLES ______________ http://www.sirinet.net/~jgjohnso/cell.html 2 KINDS of ENDOCYTOSIS Takes substances into cell If taking in: PINOCYTOSIS fluid, molecules = ____________________ PHAGOCYTOSIS large particles or whole cells = __________________ • Examples in cells: – one celled organisms eat this way – white blood cells get rid of bacteria this way ENDOCYTOSIS Animation from: http://academic.brooklyn.cuny.edu/biology/bio4fv/page/cell-movement.html http://www.accs.net/users/kriel/chapter%20nine/ PHAGOCYTOSIS White blood cell destroying germs EXOCYTOSIS Substances released outside of cell ACTIVE transport (requires energy) • __________ VESICLES • Substances move in____________ • Examples in cells: – Golgi release packaged proteins Exocytosis http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBooktransp.html GOLGI BODIES USE EXOCYTOSIS Animation from: http://www.franklincollege.edu/bioweb/A&Pfiles/week04.html Endocytosis & Exocytosis http://grossmont.gcccd.cc.ca.us/cmilgrim/Bio120/Outline/ Outline2.gif/TransportMedia/EndoExocytosisMovie.htm