Download Screening of Filamentous Fungi Producers of Xylanase

SBBq
XL Annual Meeting of Brazilian Biochemistry and Molecular Biology Society
th
rd
Foz do Iguaçu, PR, Brazil, April 30 to May 3 , 2011
Screening of Filamentous Fungi Producers of Xylanase
Oliveira, A.T.P., Sorgatto, M, Guimarães, N.C.A., Dias, K.J.C., Silva, P.O., Lima,
E.E., Zanoelo, F.F., Possiede, Y.M., Oliveira, C.S., Marques, M.R. and Giannesi,
G.C.
1
Laboratory of Biochemistry, DMF/CCBS/UFMS, Campo Grande-MS;
Xylan is the major constituent of hemicellulose which is the second most abundant
renewable resource on earth. The importance of microbial xylanases has
increased in present scenario due to its immense biotechnological applications in
mainly food, animal feed, paper and pulp industries. Efforts on cost reduction have
directed towards increasing enzyme production by developing better microbial
strains, efficient fermentation techniques and recovery systems. The purpose of
this study was to collect and isolate filamentous fungi from soil and humus of
different regions in Mato Grosso do Sul, and evaluate the production of xylanase.
The results revealed that among the 26 isolated tested, 16 (61,5%) could produce
clear zones as a result of xylan utilization after 48h of incubation. Among these 16
isolated, in SR medium the enzymatic activity was the highest for Aspergillus niger
(9.38U/mg protein) and Aspergillus terreus (8.04U/mg protein); while Aspergillus
flavus (12.43U/mg protein) and Emericella sp. (6.62U/mg protein) showed increase
of enzymatic activity in Khanna medium. M5 and M8 medium didn’t induce xylanase
production. This study contributes to catalogue soil fungi isolated in the state of
Mato Grosso do Sul, and provides additional information to support future research
about the industrial potential of these microorganisms.
Keywords: screening, filamentous fungus, xylanase.
Supported by Fundect and CNPq
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