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PREFACE This book is compiled for all aspirant scientists and teachers young at heart!!! The CAPS-exam guidelines have been followed. This book is designed to help students to understand Life Sciences and make it their own. To my critical fellow educators: Do not let the simplicity fool you. The academic standard was not lowered!! Compiled by: J Grobler Published by: Tel: 074 278 8623 / 084 808 9606 Fax: 086 596 1071 Email: [email protected] a Warning!!! Copyright applies to this publication. In terms of the South African copyright law any person who copies this publication, or any part thereof, or make copies, commit a crime and run the risk of being fined or put in jail. CONTENTS TERM 1 DIVERSITY, CHANGE AND CONTINUITY 1. BIODIVERSITY AND CLASSIFICATION OF MICRO-ORGANISMS 1.1 Micro-organisms 1 - Viruses - Protista - Bacteria - Immunity - Fungi - Biotechnology - brewing of beer 1.2 9 13 18 29 35 Biodiversity of plants - Bryophytes - Pteridophytes - Gymnosperm - Angiosperm 1.3 37 49 55 62 Biodiversity in the animal kingdom - Classification and phylums - Porifera - Cnidaria - Platyhelminthes - Annelida - Arthropoda - The roll of invertebrates in agriculture and ecosystems - Chordata 72 78 80 84 87 90 93 94 TERM 2 LIFE PROCESSES IN PLANTS AND ANIMALS 1. ENERGY TO SUSTAIN LIFE 1.1 Photosynthesis - Factors essential for photosynthesis - The dorsi ventral leaf - The chemistry of photosynthesis - The importance of photosynthesis - Photosynthesis experiments 102 103 105 107 108 1.2 Nutrition in mammals - Herbivores, carnivores and omnivores - Structure of the human digestive system - Homeostatic control of blood sugar - Mechanical and chemical digestion - Dietary supplements, alcohol and drugs 1.3 117 117 124 125 129 Cellular respiration - Aërobic respiration and Anaërobic respiration - The chemistry of cellular respiration - Cellular respiration experiments 139 141 144 TERM 3 LIFE PROCESSES IN PLANTS AND ANIMALS CONTINUED 2. GASEOUS EXCHANGE 2.1 Gaseous exchange in organisms - In plants and animals - Effective gaseous exchange area 2.2 The human respiratory system - The human lung - Gaseous exchange in the lungs and tissue - Respiratory diseases 3. EXCRETION IN THE HUMAN 3.1 Excretion in different organs - Lungs, kidneys, liver, skin and digestive system 3.2 150 151 152 156 158 165 The human urinary system - Composition of urine - The functioning of the nephron - Homeostatic control of water and salts - Diseases of the kidney 166 170 171 183 ENVIRONMENTAL STUDIES 1. POPULATION ECOLOGY / POPULATION DYNAMICS 1.1 Definitions - Populations, niches, biomass, parameters 1.2 186 Population size - Factors influencing population size - Population growth curves 187 190 1.3 Interaction in the environment - Competition, speciation and predation 192 1.4 Determining population size 198 1.5 Symbioses - Commensalism, mutualism and parasitism 1.6 Social organisation - Social behaviour, polymorfism 1.7 209 Ecological succession - Primary and secondary succession 1.8 205 213 Human population - Population growth, Age and gender 215 TERM 4 ENVIRONMENTAL STUDIES CONTINUED 2. HUMAN IMPACT ON THE ENVIRONMENT 2.1 The atmosphere and climate change - Release of carbon dioxide - Ecological and carbon foot print - Deforestation and the greenhouse effect - Methane release and ozone layer 2.2 Water - Availability of water, dams - Wetlands and poor farming methods - Alien plantations, bore holes, water wastage - Water quality, mining activities and water pollution 2.3 2.5 222 223 224 225 Food security - Poor farming methods, pest control - Soil erosion and fertilizers, alien plants - Genetic manipulation, impact on gene pools - Wastage 2.4 217 217 218 220 228 230 231 232 Loss of bio diversity - Habitat destruction, poaching and indigenous knowledge 233 The processing of solid waste - Control of dumping sites - Recycling - Methane gas, nuclear waste 238 239 239 I GRAPHS TO BE MASTERED A. The line graph 1. 2. Always add a heading to the graph as well as a key to indicate different graphs The axis (X and Y) must be correctly calibrated (e.g. 2,4,6,8 or 3,6,9,12 etc.) - You usually have to start with a 0-value Relative constant values which are called the INDEPENDENT VARIABLES will be found on the X-axis e.g. temperature, years, time etc. These values are not easily influenced by external factors The values on the Y-axis are influenced by external factors such as those found on the xaxis, and are called the DEPENDENT VARIABLES The unit of the axis must always be indicated The data on the graph must be shown clearly 3. 4. 3. 4. X-axis ( Temperature in °C) 20 30 35 40 45 50 Y-axis ( Reaction rate in mg/min) 2 3,5 4,5 5,5 5,5 1,2 Reaction rate of enzyme (mg/min) Temperature (°C) B. The Pie graph 1. The total area represents 100% 2. The sum of the segments will equal 100 % II C. The Histogram 1. 2. 3. 4. Data is represented by individual columns for comparison A series of data is shown The columns touch and are of equal width The axis must be correctly calibrated Number of deaths Age at death (years) D. The bar graph 1. 2. The bar graph has spaces between the columns Each column represents something different, and no series is formed Composition (%) KEY: 1 DIVERSITY, CHANGE AND CONTINUITY BIODIVERSITY MICRO-ORGANISMS 1. 2. 3. 4. Micro organisms / microbes are organisms that can only be seen through a microscope The micro organisms contribute to biodiversity in ecosystems, since millions are present in important food chains Micro organisms play a daily role in - Diseases, e.g., tuberculosis, AIDS, cholera, malaria etc. - Food production, e.g., yeasts in baking, production of wine and cheese etc. - Decaying of food by fungi and bacteria - Sewage disposal by bacteria These include: - viruses that range in size from 0,01 um to 0,1 um ( 1 um = 0,001 mm) - bacteria that range between 0,1 um to 5 um - Protista - unicellular animals such as Amoeba and - those causing diseases such as Malaria - fungi - bread mould and mushrooms VIRUSES INTRODUCTION 1. Viruses can be seen as inert or dormant. They only become active when they enter living cells of other organisms. They are therefor not considered as living organisms at all by some scientists! 2. They are all, without exception, parasites or pathogenic, since they cause diseases 3. In 1918, 20 million people died of a flu epidemic in Europe - more than in world war one! 4. Viruses only have one function, and that is to REPRODUCE! - All other metabolic activities are absent! 5. Viruses are transported passively by air, water, physical touch, body fluids and food THE STRUCTURE OF VIRUSES 1. They are extremely small and are only visible under very high magnification of electron microscopes 2. The body consists of - a protein coat / capsid consisting of capsomeres which enclose - a nucleic acid 3. The nucleic acid is - RNA in viruses found in plant cells - DNA in viruses found in animal cells 4. They range from cylindrical to spherical (round) To Contents 2 The influenza (flu) virus The tobacco mosaic virus RNA Capsomere Helix DNA Capsid Typical structure of an animal virus A cubic wart virus Capsomere DNA/RNA A membrane envelope The structure of a bacteriophage = a virus pathogenic on bacteria 3 REPRODUCTION / REPLICATION IN VIRUSES 1. 2. 3. 4. 5. 6. 7. 8. 9. Viruses can only reproduce inside plant or animal cells The virus attaches to the cell and injects its nucleic acid into the cell The protein capsid stays on the surface of the cell Inside the cell, the virus DNA / RNA takes control of the host cell’s DNA / RNA This changes the chromosomes of the cell and can be seen as a form of mutation! The virus-DNA/RNA now controls the cell The cell is controlled to produce new viruses As soon as the cell is exhausted and filled with new viruses, it bursts open and releases the new viruses The newly formed viruses now attack other cells in the organism’s body Replication of bacteriophage viruses The virus attaches to host cell New viruses released Bacterium DNA forced inside - now controls the host DNA Host cell filled with new viruses Host cell produces new viruses BIOLOGICAL IMPORTANCE OF VIRUSES Viruses cause many diseases in plants, animals and humans IN PLANTS 1. 2. Abnormalities in plants cause massive economical losses The tobacco mosaic virus in tobacco leaves cause CHLOROSIS in the leaves - The leaves turn yellow since chlorophyll is absent - This makes the tobacco leaves useless for the market 4 SPREADING OF VIRUSES 1. Viruses are spread by - food and water - contact = coughing, sneezing and sexual contact - vectors (carriers) such as mosquitos, flees, plant lice and ticks - Until now the HIV-virus is not transmitted by mosquitoes! 2. If a person comes in contact with a virus, he builds up an immunity against the virus by - producing anti bodies which “recognise” and help to eliminate the virus - That is why babies are vaccinated against different diseases - The vaccine contains a weakened strain of the virus - the baby is actually infected with the virus - to enable him/her to produce antibodies against the particular virus 3. Plants are cultivated to make them resistant against certain viruses DISEASES CAUSES BY VIRUSES (VIRAL DISEASES) DISEASE 1. Rabies Caused by the Rabdo virus 2. AIDS Caused by the MIV-virus A spherical retrovirus 3. Colds and flu 4. German measles / Rubella TRANSMISSION AND SYMPTOMS The animal is infected by the bite of another rabid animal. Viruses are transmitted by saliva of a rabid animal. The virus attacks the central nervous system and other organs The infected animal appears tamed with a foaming mouth. It develops hydrophobia-fear of water. Hallucinations and confusion are found in infected humans. Aids = Acquired immune deficiency syndrome The virus is transmitted through blood, sexual contact and from a mother to her unborn baby. The virus attacks the immune system, by reducing the T-cells that protect the body against infections. The person dies of other opportunistic diseases such as TB and pneumonia etc. Infection occurs during low immunity through direct contact with the virus. E.g.. Contact with infected hands by sneezing, coughing, nose blowing etc. Causes an abnormal baby if a woman is infected in the first four months of her pregnancy. An abortion is performed. TREATMENT Louis Pasteur developed a vaccine against rabies. Pets are inoculated every six months. Immunisation – weakened viruses are injected into the body. Antibodies are produced against the virus to protect the body against the disease in the future Anti-retroviral drugs are used. No treatments in the advanced stages are available. Prevention is the only solution to this disease! Drink lots of water and rest! Only the symptoms are treated Immunisation is possible Immunisation is possible for pregnant women. A girl must be infected to build natural immunity against the virus. 5 EFFECT AND MANAGEMENT OF AIDS IN SOUTH AFRICA A. THE STAGES OF AIDS AFTER INFECTION WITH THE HIV-VIRUS 1. Primary HIV infection - The virus migrates to the lymph nodes and many new HIV viruses are produced Sero-conversion - Antibodies against the HIV virus are produced - Flu-like symptoms, a skin rash and swollen lymph nodes are present - Night sweats can occur - The person will test positive for HIV antibodies - The person is now HIV-positive – all other symptoms disappear - This stage can last between a few weeks and 13 years! Opportunistic infections will be experienced - Immune system decline will now occur - Aids-related diseases such as viral, bacterial and fungal infections will appear - Weight loss will also take place - A lowered T-cell count is found (fewer T-cells) Aids-related diseases become more severe - Organs are attacked and weight loss becomes severe - Diarrhoea, fever and fatigue occurs - The person dies of other diseases such as pneumonia, tuberculosis etc. 2. 3. 4. B. AIDS STATISTICS / NUMBERS WHY ARE AIDS STATISTICS OFTEN INACCURATE? 1. 2. 3. 4. 5. Many cases are not reported, only specific people are tested Many people are not aware that they are HIV positive and they are in the window period Many people that are tested could have emigrated or they could have died Mistakes are made in the composition of statistics Statistics are often outdated and therefore inaccurate FACTORS DECREASING AIDS NUMBERS 1. 2. 3. 4. 5. 6. More condoms are freely available Successful training programs are held and consciousness on HIV-spreading increases Persons are more loyal to their lovers Abstinence (no sex) becomes fashionable More people die of AIDS and are not part of the statistics anymore Fewer mothers transmit the virus to their babies FACTORS INCREASING AIDS NUMBERS 1. 2. 3. 4. 5. Increase in licentiousness - more people become sexual active at a young age Unfaithfulness - having more than one lover People are uninformed - they don’t believe the HIV-virus can kill People in desolated areas - people don’t have access to condoms and clinics Training programs and informing people on the HIV-virus are insufficient 6 SHOULD THE GOVERNMENT TEST ALL PERSONS AND THEN ANNOUNCE THEIR HIVSTATUS? A. ADVANTAGES CONNECTED TO IT 1. 2. 3. 4. 5. Announcing a person’s HIV-status can enable his/her lover to protect her-/himself Planning for medical care can be done by the government and other companies. Budgets can be planned ahead Infected persons can be helped to prevent further infections Couples will be loyal to each other More accurate statistics will be available B. DISADVANTAGES CONNECTED TO IT 1. 2. 3. 4. 5. Individuals’ rights to their own privacy are violated Information can be misused and employers can discriminate against HIV-positive workers Discrimination and rejection by friends and colleagues can occur Suicide can increase under HIV-positive individuals The costs for testing the whole population are very high ACTIVITY VIRUSES 1. Why is it illegal to take meat products such as biltong as well as fresh fruit and vegetables through customs on an airport when you leave or enter a foreign country? ________________________________________________________________________ 2. Will it one day be possible for the HIV-virus to be transmitted by mosquitoes? ________________________________________________________________________ 3. Can a virus which is pathogenic on plants, also cause diseases in humans? ___________ 4. Why must you wash your hands after blowing your nose? _________________________ _______________________________________________________________________ 5. Where did the saying “Bless you?!” still used today when someone sneezes, originate? (Use the internet) _________________________________________________________ 6. How are viruses transported inside? a) animals _______________________ b) plants ________________________________ 7. What is meant by “mutations”? Can it be a possible explanation for why you can be infected with influenza every year? ___________________________________________ _______________________________________________________________________ 8. 7 Study the information on AIDS as supplied by the South African Health Department and answer the questions that follow: Table 1: The percentage of HIV-positive women that visited prenatal clinics in South Africa Age < 20 20 – 24 25 – 29 30 – 34 35 – 39 40 – 44 Table 2 1999 16,5 25,6 26,4 21,7 16,2 19,5 8.1 2001 15,4 28,4 31,4 25,6 19,3 26,2 2002 14,8 29,1 34,5 29,5 19,8 26,4 2003 15,8 30,3 35,4 30,9 23,4 25,8 2001 2,5 million 2,2 million 106 109 4.7 million 2002 2,95 million 2,3 million 91 271 5,3 million 2003 3,1 million 2,4 million 96 228 5.6 million Sex 2004 3,3 million 2,8 million 104 963 6,29 million The percentage of HIV-positive individuals that were tested at clinics in South Africa Where is the highest percentage of HIV-positive people found? GROUP Age 2004 16,1 30,8 38,5 34,4 24,5 17,5 The number of HIV-positive individuals that were tested at clinics in South Africa Group Women Men Babies TOTAL Graph 1: 2000 16,1 29,1 30,6 23,3 15,8 23,1 ANSWER POSSIBLE EXPLANATION 8.2 8 Where is the lowest percentage of HIV-positive people found? GROUP Age ANSWER POSSIBLE EXPLANTATION Sex 8.3 Why does the occurrence of AIDS decrease in higher age groups? ________________________________________________________________ ________________________________________________________________ 8.4 What is the best solution to decrease the occurrence of AIDS in South Africa? ________________________________________________________________ ________________________________________________________________ 8.5 Debate the following statement: “Condoms should be freely available in the bathrooms of schools” _____________________________________________ ________________________________________________________________ ________________________________________________________________ 8.6 How is the AIDS virus transmitted to babies? ____________________________ ________________________________________________________________ 8.7 Do you think that antiviral medications should be freely available to everybody? Give a reason for your answer _______________________________________ ________________________________________________________________ 8.8 In 2004 60 million people in the world were HIV-positive. Which percentage of this total was found in South Africa? Show all your calculations! ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ 9. On billboards on the highway is the following slogan: “If you love me, protect me! Use a condom!” Create your own slogan to make people aware of the dangers of AIDS 9 PROTISTA 1. 2. 3. Protista consists of organisms which are eukaryotic, since they have true nuclei and cell organelles inside a cellular membrane The Amoeba is an example of one of the simple Protista and is found in the muddy sediment of clean fresh water ponds Other examples are green algae such as Spirogyra and Euglena that can photosynthesize and which are also found in clean fresh water ponds. The Euglena has flagella to swim with (Is it a plant-like animal or an animal-like plant??) Spirogyra 4. 5. 6. Euglena Many Protists are pathogenic since they cause diseases such as: - Plasmodium sp. that cause Malaria which is transmitted by the Anopheles mosquito - Trypanosoma sp. that cause sleeping sickness and is transmitted by the Tsetse fly The mosquito and Tsetse fly act as vectors since they transmit the parasite The symptoms of Sleeping sickness are a high fever, anemia, sleepiness and then death To Contents MALARIA 1. 2. 3. 4. 5. 6. 7. 10 Malaria is a tropical disease that is found in 90 countries world wide - that is caused by a group of unicellular, parasitic organisms (Protista) - which live on the red blood corpuscles (erythrocytes) - of reptiles and mammals There are four malaria species which are pathogenic on the human namely: - Plasmodium falciparum, P. ovale, P. vivax and P. malariae P. falciparum is the most dangerous and is also very common in Africa Plasmodium is transmitted by the female Anopheles mosquito - which acts as the vector Malaria kills more than one million people every year, from which 90% of the deaths are in Africa Malaria is responsible for one out of four deaths among children The chemical substance Chloroquine is the safest to use against Malaria, but is losing its effectiveness against the parasite rapidly THE LIFE CYCLE OF PLASMODIUM Two stages are found inside the human body namely - in the liver and in the erythrocytes A. The liver stage 1. The human is infected by sporozoites which are present in - the saliva of the female Anopheles mosquito The sporozoites migrate to the human liver Each sporozoite produces up to 40 000 merozoites through mitosis - which are released into the blood stream 2. 3. B. The blood stage 4. 5. Erythrocytes are invaded by the merozoites = Erytrocytic phase The destruction of the erythrocytes causes anaemia in the infected person - who feels very tired and listless Erythrocytes burst open and release merozoites into the blood stream - This produces a high fever of 40°C as well as flu symptoms Malaria can usually be detected in the blood stream during this phase The merozoites invade other liver cells and erythrocytes The kidneys, liver and milt are overloaded with toxins - which can cause death Some merozoites develop into male and female gametocytes Gametocytes are ingested with the blood by the Anopheles female mosquito - when an infected person is bitten The gametocytes develop into gametes in the gut of the mosquito Male gametes fertilize the female gametes and - Zygotes are formed which invade the gut wall The zygote undergoes meiosis and - forms a cyst in the gut wall Many sporozoites are produced from one cyst - which migrate to the salivary glands of the mosquito The mosquito can now infect the next person The parasite does not affect the mosquito in any way Up to 2 million P. falciparum parasites can be present in an infected person - and 40% of the eythrocytes can be destroyed before death occurs 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 15. 16. 17. 11 THE LIFE CYCLE OF PLASMODIUM (MALARIA PARASITE) A. IN THE HUMAN Second mosquito infected Erythrocyte produces gametocytes Male and female gametocytes ingested Merozoites invade erythrocytes and liver cells Erythrocyte produces merozoites First mosquito Start of the cycle Sporozoites injected into bloodstream and invade liver cells and erythrocytes Erythrocyte invaded by merozoites Sporozoites produce merozoites in liver cells B. IN THE MOSQUITO Gametocytes produce gametes inside gut of mosquito Male and female gametocyte ingested Male Zygote Female Sporozoites migrate to salivary glands of mosquito Many Sporozoites are produced in gut wall Migrates to gut wall of mosquito Cyst forms in gut wall ACTIVITY 12 MALARIA Study the following information on Malaria and answer the questions: 1. Malaria is combatted by the following: - the use of drugs such as chloroquine that prevent Malaria - the spraying of water masses with insecticides where mosquitoes hatch - the use of mosquito nets over beds - the spraying of insecticides inside homes - the drainage of water masses where mosquitoes hatch - the use of fish that feed on mosquito larvae - the development of a natural immunity in people that are often infected 2. The graph illustrates the occurrence of malaria in South-Africa between 1989 and 2004, as supplied by the Medical Research Unit of South Africa in an informal settlement. Number of females Number of males 1. Which method of combat is the most harmful to the environment? Explain your answer _______________________________________________________ ______________________________________________________________ 2. Which method of combat is the most environmentally friendly? Explain! ______________________________________________________________ 3. Why are more women infected than men? ____________________________ ______________________________________________________________ 4. What caused the abnormal high infection rate between 1998 and 2000? Keep in mind where mosquitoes breed! ______________________________ ______________________________________________________________ 5. Do you think that a person is more vulnerable to malaria if he is infected with AIDS? Give a reason for your answer _______________________________ ______________________________________________________________ 13 BACTERIA INTRODUCTION 1. 2. 3. Before antibiotics were developed by Alexander Fleming in 1929 - the human race was affected greatly by a vast number of bacterial diseases Thousands of soldiers died of infections in their wounds before antibiotics were used Alexander Fleming experienced world war one as well as the second world war. His antibiotics were only used in the second world war to save thousands of lives (Can you imagine the pain and hardship during world war I ?) Interesting facts Louis Pasteur witnessed an attack by a rabid dog on the inhabitants of his hometown in Southern France when he was only nine years old, which influenced him greatly. He later qualified as a microbiologist. He did valuable experimental work on viruses as well as bacteria. Use the Internet or other resources to find out more about his work PASTEURIZATION 1. 2. 3. 4. Louis Pasteur is the “father” of pasteurization which is still - used today to keep our milk fresh for longer His first experiment was on wine which he boiled and sealed - to prove that the bacteria responsible for the wine to ferment, - were found in the surrounding environment Milk is heated for 15 seconds at 72°C and then cooled - immediately during the pasteurization process This process kills all the bacteria in the milk THE STRUCTURE OF A BACTERIAL CELL 1. 2. 3. 4. 5. 6. Bacteria are unicellular organisms that form colonies Bacteria have the basic structure of a plant cell with: - a cell wall consisting of cellulose = polysaccharide - a slime sheath/capsule that surrounds the cell wall and protects the cell from drying out - a cell membrane which is selectively permeable - a granular protoplasm with vacuoles and dissolved substances NO nucleus is present - only chromatin granules consisting of DNA are present - which makes the cell prokaryotic Food granules are found in the protoplasm 1 to more flagella help with locomotion Hair-like structures / pili are found on the external surface of the cell - which help the bacterial cell to attach to its host - e.g the colon bacillus attaches to the gut of humans by its pili To Contents 14 THE STRUCTURE OF A BACTERIAL CELL Flagellum Slime capsule Mitochondrion Cell wall Cell membrane Chromatin material (DNA) Cytoplasm Vacuole Food granules 5. Bacteria are found in different forms: A. Coccus / spherical B. Bacillus / rod-shaped Monococcus Monobacillus e.g tuberculosis Diplococcus e.g pneumonia Diplobacillus Streptococcus e.g sore throat + food poisoning Staphylococcus e.g boils C. Spirillum / spiral shaped Bacillus with flagella e.g syphilis 15 REPRODUCTION IN BACTERIA 1. 2. 3. 4. 5. Budding : A new bacterial cell grows from the mother cell Endospores: The slime capsule forms a thick-walled structure that is able to survive extremely adverse conditions. This is also a dormant/resting stage Segmentation : The bacterial cell breaks into small segments. Each can survive individually Conjugation : Two bacterial cells exchange genetic material (DNA/RNA) in order to survive - They use their pili to attach to each other Binary fission /Mitosis: This is the most common and fastest means of reproduction. That is why bacterial diseases and infections spread so rapidly! Binary fission / mitosis in a bacterial cell Two identical daughter cells are formed Mother cell divides by mitosis THE ECOLOGICAL ROLE OF BACTERIA A. BENEFICIAL (USEFUL) BACTERIA A1. In food 1. 2. 3. Anaerobic bacteria (Requires no oxygen) convert sugar during fermentation to - alcohol and carbon dioxide This process is used in the production of wine, medicine and other alcoholic beverages such as - brandy and ginger beer! Beneficial bacteria are also used in the production of yoghurt, buttermilk and cheese A2. As Decomposers / saprophytes 1. 2. 3. 4. Dead organisms are decomposed to again become part of the ecosystem Roughage in the colon of the human is digested by millions of bacteria Bacteria help with the digestion of plant material in herbivores E.coli bacteria in the human colon help with the production of vitamin K which is necessary for blood clotting - This symbiosis is seen as mutualism, - since both organisms benefit from it 16 A3. In the nitrogen cycle: 1. Nodular bacteria on legume roots (beans, soya beans and peas) - fix nitrogen from the atmosphere in the soil - for plants to build amino acids and proteins 2. These plants are sources of nitrogen for animals and humans - for the production of amino acids - to build proteins in their own bodies - e.g enzymes, hormones, protoplasm of cells etc. 3. Nitrifying and denitrifying bacteria convert nitrates and - ammonia (from urine and faeces) - to free nitrogen in the atmosphere (Done in grade 10!) B. HARMFUL BACTERIA In the food industry: 1. Bacteria found in spoilt food, can cause serious illnesses 2. The bacteria cause the food to “decompose”! 3. This causes food poisoning which can cause death if not treated Pathogenic bacteria USE THE TABLE ON THE FOLLOWING PAGE!