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DGL, active molecules carrier Characteristics of a DGL • • • • • • DGL : Dendri-Graft Poly-Lysine Spherical-shaped nanoparticle Wide range of sizes (1 to 8 nm radius from G1 to G5) 100% composed of essential amino acids (L-Lysine) Many amine functions on surface 80 % of free volume (flexible tree-like generated structure) Added value of Colcom’s DGL Natural biocompatibility Non-immunogenic Water soluble (cationic DGL) Easily customizable High strucural flexibility Reproducibility of production High production capacity Controlled narrow poly-dispersity Capture of metals (Ag+, Co2+, Cu2+, Zn2+, Ni2+) DGL : a tool for various transports Depending on the applications you are looking for, we construct a DGL (size, charge, hydrophobicity) that will lead to specific behaviors and respond precisely to your needs. NH2 DGL bearing a reactive function: maleimide, thiol, acetylenic or azido in order to graft the bioactive molecule. Tagged DGL: by a fluorophore (fluorescein) or a chromophore (erythrosine) for the localization or the detection. Chemical modification of DGL’s surface: conversion of a cationic surface into an anionic, neutral, hydrophilic or hydrophobic surface. Modification of surface amino acids: replacement of lysine by another amino acid. NHS SH HOOC O NH OH N3 NH2 N 2 N3 3) Cibles (compilation) T T T T 1 Tag inside Tags outside Combination of different modifications: grafting on the same DGL of bioeactive molecules, specific ligands, fluorophores and PEG chains for a DDS (Drug Delivery System). Applications Drug delivery: solubilization, transport and targeting of bioactive molecules (drugs, proteins, peptides, ADN, siRNA…). Crossing through the blood-brain barrier. In vivo or in vitro diagnostic : Fluorophores markers: detection and localization of in vivo DGL Capture of MRI contrast agents (increases the contrast and reduces the toxicity) Metal catalysis : solubilization and stabilization of catalysts. Biomolecules encapsulation : the DGL is used as structural architecture for encapsulation of biomolecules under a silica layer in order to stabilize and protect them. Immunization booster: the DGL is used as a hapten carrier in second immunization and allows the production of antibodies specifically directed against a target by significantly reducing the production of antibodies against the carrier protein used in primo immunization. Tech2Market - www.tech2market.fr [email protected] +33 (0)1.46.71.93.34 Gd Gd Gd Gd Gd Gd Gd Gd Gd Gd Gd Gd Gd Gd Gd Gd Gd Gd