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SPECIAL SENSES Overview In this exercise you will conduct a brief review of eye and ear anatomy. You will also work with a partner to perform multiple special senses tests. The lab consists of three special senses; 1) Vision 2) Hearing 3) Equilibrium Introductory Notes for Vision The innermost tunic of the eye is the retina. The retina has a neural tissue layer composed of two types of photoreceptors, rods and cones. Photoreceptors convert light energy into a nerve impulse. These nerve impulses will be transmitted to the optic cortex in the brain resulting in visual perception. Theses photoreceptors are distributed over the entire retina except for where the axons of the ganglion cells exit through the optic nerve. This area is called the optic disc and results in a blind spot. At the optic chiasm, fibers from the medial retina from each eye cross over to the opposite side and the lateral fibers of each eye remain on the same side. The fibers then extend as optic tracts and synapse with neurons in the lateral geniculate body of the thalamus and terminate in the visual cortex in the occipital lobe. Each eye receives light from both left and right hemifields. In Figure 1.1 below the yellow represents the left hemifield and the blue represents the right hemifield. Notice that each eye receives light from both hemifields. Figure 1.1 1 When light rays pass from one medium to another, their speed of transmission changes and the rays are bent, or refracted. Light travels faster in air than through other mediums such as liquid therefore; light rays slow down and are refracted when they pass through the cornea, lens, and vitreous humor of the eye to reach the retina. The cornea and the vitreous humor refractive index remains constant however, the lens refractive index, or bending power can be varied based on the lens’ shape. The greater the lens’ convexity, or bulge, the greater the light will refract. The lens does not need to change its convexity for it to focus light from a distant object, greater than twenty feet away. On the other hand, the lens becomes more convex when focusing light from a near object onto the retina. This occurs when the ciliary muscles contract, decreasing the tension on the suspensory ligament attached to the lens and allowing the lens to “plump up”. Making these adjustments is referred to as, accommodation. Both intrinsic and extrinsic muscles are needed for proper eye functioning. The intrinsic muscles of the eyes are smooth muscle and include the ciliary muscles (which alters the lens curvature in focusing), and muscles of the iris (which controls pupil size). The extrinsic muscles are skeletal muscle and attach to the outside of the eye. These muscles control eye movement and make it possible to keep moving objects focused on the fovea centralis. They are also responsible for convergence, or medial eye movement, which is essential for near vision. Visual acuity, or sharpness of vision is tested with a Snellen eye chart, which consists of letters of various sizes printed on white paper. This test is based on the fact that letters of a certain size can be seen clearly by normal vision at a specific distance. Astigmatism is a condition resulting is unequal curvature of either the cornea or lens. This condition prevents light rays from being focused with equal sharpness on the retina. The astigmatism chart is designed to test for defects in the refracting surface of the lens and/ or cornea. The sensation of color vision depends on the degree to which impulses are formed by three types of cones (receptors for red, green, and blue) in the retina. Color blindness is more common in men than in women because the genes for the red and green color receptors are located on the X chromosome, of which males have only one and females have two. The most common type of color blindness is red- green color blindness, which is caused by a deficit in cones stimulated by either red or green light. People with this deficit have difficulty distinguishing between red and green. A completely color blind individual would see everything as shades of gray. Introductory Notes for Hearing The mechanism of hearing begins as sound waves pass through the external auditory canal to the tympanic membrane, through the middle ear, and into the inner ear, where the vibrations reach the spiral organ of Corti. This is where the mechanoreceptors for hearing are located and stimulated by sounds of various frequencies and amplitude. Once they are stimulated they depolarize and begin a chain of nerve impulses by way of the cochlear nerve to the auditory centers of the temporal lobe cortex. Tone or pitch is determined by the particular receptors that are stimulated based on the frequency of the vibrations and the part of the brain that receives it. High frequency waves result in high-pitch sounds detected close to the oval window. Low frequency waves result in low-pitch sounds detected near the apex of the cochlea. The amplitude of the sound waves, which will increase the frequency of the nerve impulses that reach the brain, determines loudness. There are two types of hearing loss. Conduction deafness results from the blockage of sound waves reaching the inner ear. This can be corrected by surgery or hearing aids and is detected with a Rinne test. Nerve deafness (a.k.a sensorineural) is caused by damage to the sounds receptors or neurons that send the impulses to the brain. This usually is a result of loud noise and is not correctable. A Weber test detects if there is difference between the left and right ear simultaneously. If conduction deafness is present in one ear, the sound will be heard more strongly in the ear with hearing loss due to bone conduction of the skull. 2 Introductory Notes for Equilibrium The equilibrium apparatus of the inner ear is in the vestibule and semicircular canals. The vestibule contains the saccule and utricle, and the semicircular canals contain the semicircular ducts. The three semicircular ducts are involved in the mechanism of dynamic equilibrium and are oriented in a horizontal, frontal, and sagittal plane. The ampulla is located at the base of each duct and houses the receptor region called crista ampullaris, which consists of hair cells covered with a gelatinous cap, or cupula. When your head position changes in an angular direction, the endolymph in the canal lags behind, pushing the cupula in the opposite direction of the movement, bends the hair cells and creates an action potential. Maculae in the saccule and utricle contain the hair cells, receptors involved in the mechanism of static equilibrium. The maculae respond to gravitational pull providing information on which way is up or down, and to linear changes in speed. The hair cells in each macula are embedded in the otolithic membrane which contains calcium carbonate (otoliths). When the head moves, the otoliths move in response to gravitational pull. Tests such as the Barany Test detect nystagmus, which is the trailing of the eyes slowly in one direction, followed by their rapid movement in the opposite direction. If the semicircular canals are operating normally one will demonstrate nystagmus after rotation; abnormal otherwise. Romberg Test is used for the clinical assessment of disequilibrium or ataxia from sensory and motor disorders. Equilibrium is maintained through proprioception, vestibular, and visual sensory input. Experimental Procedures for Vision Blind Spot Test This is a test for the presence of the blind spot or optic disc. The optic disc is the point at the back of the eyeball where the optic nerve exits. To perform this test, hold Figure 1.2 about 18 inches from your face. To test the right eye, close the left eye and stare at the plus sign with the right eye. You should be aware of the dot in the periphery of your vision. While continuing to stare at the plus sign, slowly move the page toward your face. At a certain distance, the dot will disappear from your peripheral vision as it comes into focus on the optic disc. To test the left eye, close the right eye and stare at the dot with the left eye while slowly moving the page toward your face. The plus sign will disappear from your peripheral vision when it falls on the optic disc. Answer the questions on the data sheet. Figure 1.2 3 Test For Near Point Accommodations The closest distance at which one can see an object in sharp focus is called the near point. Close one eye and look at the letter “T” at the beginning of this paragraph. Move the page closer to your eye until the letter becomes blurred; then move it away until you get a sharp image. Have your partner measure the distance from your eye to the page using the provided ruler. This distance is your near point of accommodation. Repeat this procedure with the other eye. Record your near point for each eye. Typical near points are given below. Why does the near point increase with age? Age 20 years 30 40 50 60 Near point 3.5” (9 cm) 4.5” (11.5 cm) 6.5” (16.5 cm) 20.5” (52 cm) to 33” (84 cm) Eye Reflexes Test Focusing for close vision requires that the eye make three adjustments. Two of those adjustments can be easily observed. Look at a distant object, such as a wall across the room (not a light or window). Have your partner note the size of your pupils and the position of your eyeballs. Then, focus on a pencil held about 10 inches from your face. Again, have your partner note the size of your pupils and the position of your eyeballs. Answer the questions on the data sheet. Visual Acuity Test Test your visual acuity with the Snellen eye chart. Stand 20 feet away from the eye chart, cover one eye and attempt to read the line with the smallest letters that you can see. Do not squint. Have your lab partner note the visual acuity value associated with that line on the chart. Repeat with the other eye. If you wear glasses, perform this test with and without your glasses. If you wear contacts, keep them in but note it in your documentation. Normal vision is 20/20. If your vision tested 20/50, you can see at 20 feet what a person with normal vision can see at 50 feet. Record your results in the data sheet. Test for Astigmatism Using the Astigmatism chart, stand 20 feet away from the chart, cover one eye, and focus on the circle in the center. If all the radiating lines appear equally dark and distinct, there’s no astigmatism. If some of the lines are blurred or appear less dark than others, then some degree of astigmatism is present. If this is the case, record the number (I-XII) of the line that appears light or blurred in you data sheet. Repeat the process with the other eye. Color Blindness Test Have your lab partner hold up the color blindness test plates about 30 inches from your eyes. Look at the color blindness test plate (Ishihara plates) for 3 seconds and state which number you see on the plate. Refer to the answer key which accompanies the test plates. If you are unable to read the numbers, this may indicate that you are color blind. Answer the questions in your data sheet. 4 Experimental Procedures for Hearing Rinne Test Produce vibrations in a tuning fork by holding it by the handle and striking the prongs (tines) against the palm of your hand. Do not strike it against a hard object! Do not touch the tines; hold it by the handle only. Place the handle of the tuning fork against the mastoid process of the temporal bone. When the sound is no longer audible, position the tines 1 inch from the external acoustic meatus. Ask your subject where the sound was loudest. If the sound is louder at the meatus, then hearing is not impaired. If it is louder at the mastoid process, then the subject has a degree of conduction deafness in that ear. Repeat with the other ear and record your results on the data sheet. Next, produce vibrations in a tuning fork and hold it outside of the external auditory meatus. When the sound is no longer audible, place the handle of the tuning fork against the mastoid process. If sound is again audible, some conduction deafness if present. If nothing is heard at the meatus or the mastoid process then nerve deafness is present. Weber Test Produce vibrations in the tuning fork and place the handle of the tuning fork against the top of the subject’s head. If it is equally loud in both ears, you have equal hearing or equal loss in both ears. Next, plug one ear to simulate conduction deafness and repeat the test. The sound should appear louder in the plugged ear. Answer the questions in your data sheet. Barany Test (Induction of Nystagmus and Vertigo) This evaluates the semicircular canals and should be conducted as a group effort to protect test subjects from possible injury. Before beginning this experiment, please adhere to the following. Select a subject who is not easily inclined to dizziness during rotational movements. Be sure to stop rotation immediately if the subject feels nauseated and have other group members ready to catch, hold, or support the subject and the end of the rotation as the subject will experience vertigo and loss of balance. Instruct the subject to sit on a rotating chair and to hold onto the seat or chair arms, and have the feet off the ground. The subject’s head should be tilted forward approximately 30 degrees (almost touching the chest). The horizontal (lateral) semicircular canal will be stimulated when the head is in this position. The subject’s eyes are to remain open during the test. Four classmates should position themselves so that the subject is surrounded on all sides. The classmate behind the subject will rotate the chair to the subject’s right for 10 revolutions in 10 seconds, then abruptly stop the rotation. Immediately note the direction of the subject’s nystagmus. Ask the subject to describe the feelings of movement, indicating speed and direction of sensation. Record the information on the data sheet. Role of Vision in Maintaining Equilibrium The Romberg test determines the integrity of the dorsal white column of the spinal cord, which is responsible for transmitting impulses from the proprioceptors involved in posture to the brain. Have the subject stand with their back to the whiteboard. On the whiteboard, draw a series of parallel vertical lines, about an inch apart from each other, on both sides of the subject. The subject should stand straight with their eyes open looking straight ahead. Have one lab partner stand in front and observe the subject’s degree of side-to-side movement and another to act as a spotter incase they lose their balance. Repeat the test and observations with the subject’s eyes closed. Then have the subject raise their left foot approximately 30 cm off the floor and hold there for 1 minute with eyes open. Record the observations on the data sheet. Rest for 1 minute and then repeat the experiment with the same foot raised but with eyes closed. Record the observations on the data sheet. 5 NAME: ___________________________ Lab Section:________________________ Data Collection and Follow Up Questions Visual Pathways of the Brain After examining visual pathways of the brain in figure 1.1, determine what effects lesions in the following areas would have on vision. 1. In the right optic nerve: _________________________________________________________ 2. Through the optic chiasma: ______________________________________________________ 3. In the left optic tract: ___________________________________________________________ 4. In the right visual cortex: ________________________________________________________ Blind Spot Test 1. Explain why the optic disc is a blind spot. _________________________________________________ _____________________________________________________________________________________ 2. Why are you not aware of this blind spot except when performing this test. ___________________________ _________________________________________________________________________________________ Test For Near Point Accommodations 1. Near point for right eye: ______________ inches. Is it close to average for your age? _________________ 2. Near point for left eye: _______________ inches. Is it close to average for your age? _________________ 3. Why does the near point increase with age? ____________________________________________________ _________________________________________________________________________________________ Eye Reflexes 1. List the three adjustments that took place when looking at the pencil held 10 inches away. Explain why each is necessary.____________________________________________________________________________ _________________________________________________________________________________________ _________________________________________________________________________________________ _________________________________________________________________________________________ 6 Visual Acuity Test 1. Visual acuity, right eye without glasses: _20/_______ 2. Visual acuity, right eye with glasses: _20/_______ 3. Visual acuity, left eye without glasses: _20/_______ 4. Visual acuity, left eye with glasses: _20/_______ Test for Astigmatism 1. Is there astigmatism present in the right eye? _______ If yes, what line number(s) was blurring? _________ 2. Is there astigmatism present in the left eye? _______ If yes, what line number(s) was blurring? __________ 3. What does the presence of astigmatism indicate? _______________________________________________ ________________________________________________________________________________________ Color Blindness Test 1. Is there any indication that you have some degree of color blindness? _______ 2. If so, what type? _________________________________________________________________ Rinne Test 1. State and interpret your results for the right ear. _____________________________________________________________________________________ _____________________________________________________________________________________ 2. State and interpret your results for the left ear. _____________________________________________________________________________________ _____________________________________________________________________________________ 3. If you could hear the sound from the mastoid process, but not the external auditory canal, what specifically would that indicate about your hearing?______________________________________________________ _______________________________________________________________________________________ Weber Test 1. Is the sound equal in both ears, or is it louder in one ear? _______________________________________ _______________________________________________________________________________________ 2. What did you notice when you plugged one ear using this test and why did that occur? _______________ _______________________________________________________________________________________ 7 Barany Test 1. Describe the subject’s feelings of movement, indicating speed and direction sensation. _______________ _______________________________________________________________________________________ 2. When the subject is rotated to the right, which direction does the cupula bend? ______________________ 3. Describe the nystagmus observed. Were the subject’s eyes moving more quickly to one side than the other? _______________________________________________________________________________________ Romberg Test & Role of Vision in Maintaining Equilibrium 1. Did you observe any swaying movement when the eyes were open? _______________________________________________________________________________________ _______________________________________________________________________________________ 2. Describe the degree of swaying movement observed when the eyes were closed. Compare observations to when eyes were open. ________________________________________________________________________________________ 3. Does vision play a role in equilibrium? Explain your answer based on your observations. _______________________________________________________________________________________ _______________________________________________________________________________________ 8