Download phage cycle and bacterial metabolism

L.C.B
PHAGE CYCLE AND BACTERIAL METABOLISM
Group leader: Mireille Ansaldi, Research Director
LABORATOIRE DE CHIMIE BACTERIENNE
[email protected]
04 91 16 45 85
Research in our group focuses on several aspects of
bacteriophage biology and their applications. On one hand, we
aim at elucidating host-phage interactions utilizing up to date
molecular and imaging approaches. We mainly focus on
temperate phage infection and lysogenic conversion to
understand how this shapes bacterial genome evolution and
influences the host physiology using several bacterial models
such as Escherichia coli, Salmonella enterica and Xylella
fastidiosa. The elucidation of the regulatory interplays between
prophage and host genes and the deciphering of how the host
regulatory network influences prophage gene expression and
prophage maintenance are central topics in our work.
On the other hand, we are involved in translational projects
based on the knowledge acquired on phage biology. We
develop phage-based biotechnological tools such as highly
specific biosensors that only detect live bacteria. We are also
interested in the use of virulent phages as therapeutic tools and
on their synergistic effect in combination with existing
therapeutic strategies.
Lysogenic infection
by a temperate bacteriophage
© E. Wolfson & M. Ansaldi
Bacteriophages
Lysogeny
Host-phage interact
Genetic regulation ions
Genome evolution
Biotechnologies
Phage-based tools
PUBLICATIONS
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Puvirajesinghe TM, Elantak L, Lignon S, Franche N, Ilbert M, Ansaldi M. (2012) DnaJ (Hsp40 protein) binding
to folded substrate impacts KplE1 prophage excision efficiency. J Biol Chem. 287(17):14169-77.
Panis G, Franche N, Méjean V, Ansaldi M. (2012) Insights into the functions of a prophage recombination
directionality factor. Viruses. 4(11):2417-31.
Menouni R, Champ S, Espinosa L, Boudvillain M, Ansaldi M. (2013) Transcription termination controls
prophage maintenance in Escherichia coli genomes. Proc Natl Acad Sci U S A. 110(35):14414-9.
Vinay M, Franche N, Grégori G, Fantino JR, Pouillot F, Ansaldi M. (2015) Phage-Based fluorescent biosensor
prototypes to specifically detect enteric bacteria such as E. coli and Salmonella enterica Typhimurium. PLoS
One. 10(7):e0131466.
Franche N, Vinay M, Ansaldi M. (2016) Substrate-independent luminescent phage-based biosensor to
specifically detect enteric bacteria such as E. coli. Environ Sci Pollut Res Int.
Laboratoire de Chimie Bactérienne - UMR 7283
31, Chemin Joseph AIGUIER 13009 MARSEILLE