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SHRAVIDA SHETTY [email protected] / [email protected] Tel: 7203691953 PROFESSIONAL EXPERIENCE Graduate Student – Santiago laboratory, University of Colorado, Aurora (August 2015 – Present) Research Volunteer – Dr. Rytis Prekeris laboratory, University of Colorado, Aurora (April 2013 - May 2014) Research Associate – Piramal Lifesciences Limited, Mumbai, India (January 2011 – December 2012) Medical Laboratory assistant – Kingston Hospital NHS trust, Surrey, United Kingdom (August 2008 – October 2010) SKILLS & WORK EXPERIENCE Graduate Student – Dr. Mario Santiago laboratory, University of Colorado, Aurora Proficient in molecular cloning techniques like mammalian vector design, site directed mutagenesis, DNA and RNA isolation, RT-PCR, quantitative PCR, analyzing gene sequencing results BSL-2 experience - Mammalian cell culture (T-293 and 3T3 cell lines), preparing Friend virus stocks Flow cytometry, optimizing ELISA assays, SDS PAGE and Western blotting, measuring plasma viral loads and proviral DNA Laboratory animal work - Mice genotyping using tail DNA, tissue (spleen and bone marrow) extraction and cardiac punctures Research Volunteer - Dr. Rytis Prekeris laboratory, University of Colorado, Aurora Performed shRNA knockdown techniques, protein expression and purification, mammalian cell culture and immunohistochemistry techniques using FITC and DAPI Soft Agar assays in order to detect metastatic cellular transformation Research Associate – Piramal Lifesciences Limited, Mumbai, India Design and execution of pre-clinical trials to test the anti-tubercular activity of an investigational new drug (IND) molecule Biosafety level 3 laboratory work experience – Preparation of mycobacterium growth media, Acid fast staining, sub – cultured multidrug resistant Mycobacterium.tuberculosis Laboratory animal work – Developed tuberculosis animal infection models, animal tissue extraction, intra-nasal, intra-peritoneal and oral drug administration Laboratory management – Supervision of the animal facility staff, performed QA/QC checks in the BSL-3, managed laboratory inventory and equipment maintenance schedules, maintained SOPs Medical Laboratory Assistant - Kingston Hospital NHS trust, Surrey, United Kingdom Data entry into LIMS (Laboratory Information Management System softwares) Performed blood sample processing and pre-treatment, gram’s staining and patient MRSA nasal swab and High vaginal swab test Prepared microbiological media and practiced aseptic microbiological techniques 1 Detection of bacteremia or fungemia in patient blood samples using blood culture systems EDUCATIONAL QUALIFICATIONS MSc. Biomedical Research, Virology August 2014 - 2016 (expected) University of Colorado, Aurora MSc. Biomedical Science September 2007- 2008 University of Westminster, London, United Kingdom July 2004 – 2007 BSc. Biomedical Science University of Central Lancashire, Preston, United Kingdom ACHIEVEMENTS Piramal Lifesciences – The IND molecule was approved for the bio-analytical testing phase in the trial Abstract presented at the International Conference for the European Iron Club, in St Gallen, Switzerland on September 18, 2008 Co-expression of both, TbpA and TbpB components of the human transferrin binding receptor of Neisseria meningitidis, in E. coli. Shravida Shetty+, Maryam Amini+, Robert W. Evans$, Sebastien Farnaud+. School of Biosciences, University of Westminster, London, UK; $Biosciences, School of Health Sciences and Social Care, Brunel University ADDITIONAL SKILLS & ATTRIBUTES Forming scientific hypotheses and testing them experimentally Problem solving and good communication skills Proficient in MS Office, graph pad prism, Filemaker pro, and digital tools used for molecular cloning like MacVector, Sequencher and Expassy Currently, volunteering as an English tutor at the local county library (Douglas County libraries) REFERENCES 1. Dr. Mario Santiago, Associate Professor, Division of Infectious Diseases, University of Colorado, Denver Tel: 303-724-4946 Email: [email protected] 2. Dr. Rytis Prekeris, Associate Professor, Department of Cell and Developmental Biology, University of Colorado, Denver Tel: 303-724-3410 Email: [email protected] 3. Tiffany Curtin, Adult Literacy Specialist, Douglas County Libraries Tel: 303-688-7646, Email: [email protected] 2