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SHRAVIDA SHETTY
[email protected] / [email protected]
Tel: 7203691953
PROFESSIONAL EXPERIENCE
Graduate Student – Santiago laboratory, University of Colorado, Aurora (August 2015 – Present)
Research Volunteer – Dr. Rytis Prekeris laboratory, University of Colorado, Aurora (April 2013 - May
2014)
Research Associate – Piramal Lifesciences Limited, Mumbai, India (January 2011 – December 2012)
Medical Laboratory assistant – Kingston Hospital NHS trust, Surrey, United Kingdom (August 2008 –
October 2010)
SKILLS & WORK EXPERIENCE
Graduate Student – Dr. Mario Santiago laboratory, University of Colorado, Aurora

Proficient in molecular cloning techniques like mammalian vector design, site directed mutagenesis,
DNA and RNA isolation, RT-PCR, quantitative PCR, analyzing gene sequencing results

BSL-2 experience - Mammalian cell culture (T-293 and 3T3 cell lines), preparing Friend virus stocks

Flow cytometry, optimizing ELISA assays, SDS PAGE and Western blotting, measuring plasma viral
loads and proviral DNA

Laboratory animal work - Mice genotyping using tail DNA, tissue (spleen and bone marrow) extraction
and cardiac punctures
Research Volunteer - Dr. Rytis Prekeris laboratory, University of Colorado, Aurora

Performed shRNA knockdown techniques, protein expression and purification, mammalian cell culture
and immunohistochemistry techniques using FITC and DAPI

Soft Agar assays in order to detect metastatic cellular transformation
Research Associate – Piramal Lifesciences Limited, Mumbai, India

Design and execution of pre-clinical trials to test the anti-tubercular activity of an investigational new
drug (IND) molecule

Biosafety level 3 laboratory work experience – Preparation of mycobacterium growth media, Acid fast
staining, sub – cultured multidrug resistant Mycobacterium.tuberculosis

Laboratory animal work – Developed tuberculosis animal infection models, animal tissue extraction,
intra-nasal, intra-peritoneal and oral drug administration

Laboratory management – Supervision of the animal facility staff, performed QA/QC checks in the
BSL-3, managed laboratory inventory and equipment maintenance schedules, maintained SOPs
Medical Laboratory Assistant - Kingston Hospital NHS trust, Surrey, United Kingdom

Data entry into LIMS (Laboratory Information Management System softwares)

Performed blood sample processing and pre-treatment, gram’s staining and patient MRSA nasal swab
and High vaginal swab test

Prepared microbiological media and practiced aseptic microbiological techniques
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
Detection of bacteremia or fungemia in patient blood samples using blood culture systems
EDUCATIONAL QUALIFICATIONS
MSc. Biomedical Research, Virology
August 2014 - 2016 (expected)
University of Colorado, Aurora
MSc. Biomedical Science
September 2007- 2008
University of Westminster, London, United Kingdom
July 2004 – 2007
BSc. Biomedical Science
University of Central Lancashire, Preston, United Kingdom
ACHIEVEMENTS

Piramal Lifesciences – The IND molecule was approved for the bio-analytical testing phase in the trial

Abstract presented at the International Conference for the European Iron Club, in St Gallen,
Switzerland on September 18, 2008
Co-expression of both, TbpA and TbpB components of the human transferrin binding
receptor of Neisseria meningitidis, in E. coli. Shravida Shetty+, Maryam Amini+, Robert W. Evans$,
Sebastien Farnaud+. School of Biosciences, University of Westminster, London, UK; $Biosciences,
School of Health Sciences and Social Care, Brunel University
ADDITIONAL SKILLS & ATTRIBUTES

Forming scientific hypotheses and testing them experimentally

Problem solving and good communication skills

Proficient in MS Office, graph pad prism, Filemaker pro, and digital tools used for molecular cloning
like MacVector, Sequencher and Expassy

Currently, volunteering as an English tutor at the local county library (Douglas County libraries)
REFERENCES
1. Dr. Mario Santiago, Associate Professor, Division of Infectious Diseases, University of Colorado, Denver
Tel: 303-724-4946
Email: [email protected]
2. Dr. Rytis Prekeris, Associate Professor, Department of Cell and Developmental Biology, University of
Colorado, Denver
Tel: 303-724-3410
Email: [email protected]
3. Tiffany Curtin, Adult Literacy Specialist, Douglas County Libraries
Tel: 303-688-7646, Email: [email protected]
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