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Transcription and Translation Transcription (Synthesize RNA) Translation (Use the RNA to synthesize Proteins) First Step Transcription (Synthesize RNA) A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A| U| C| C| G| U| A| G| A-T G-C G-C C-G U| C-G A-T C| TA TA CG A| C| G| U| A| G| RNA Polymerase AGGCCATTC- T C C G A| U| G T A A G C| C| G| U| A| G| RNA Polymerase A- T G- C G- C CG U| CG AT C| TA TA CG A| C| G| U| A| G| RNA Polymerase A- T G- C G- C CG U| CG AT C| TA TA C - G| G A| C| G| U| A| G| RNA Polymerase A- T G- C G- C CG U| CG AT C| TA T - A| A C - G| G A| C| G| U| A| G| RNA Polymerase A- T G- C G- C CG U| CG AT C| T - A| A T - A| A C - G| G A| C| G| U| A| G| RNA Polymerase A- T G- C G- C CG U| CG A - U| T C| T - A| A T - A| A C - G| G A| C| G| U| A| G| RNA Polymerase A- T G- C G- C CG U| C - G| G A - U| T C| T - A| A T - A| A C - G| G A| C| G| U| A| G| RNA Polymerase A- T G- C G- C C - G| G U| C - G| G A - U| T C| T - A| A T - A| A C - G| G A| C| G| U| A| G| RNA Polymerase A- T G- C G- C CG CG AT TA TA CG A-T G-C G-C C-G C-G A-T T-A T-A C-G Transcription is done. Translation is starting. But First… Questions to answer… 1) Which enzymes are used in Transcription? What is their function? 2) Where in the cell does Transcription take place? 3) During which part of the cell cycle does Transcription take place? … Why? 4) What is the end result of Transcription? 5) Why is Transcription necessary for the cell? 6) Where do the the RNA nucleotides come from? 7) What is different from the DNA nucleotides and the RNA nucleotides? 8) How is DNA structurally different from RNA? 9) What are the different types of RNA that are transcribed? What are they used for? Now… Translation (Protein Synthesis) A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G A-T G-C G-C C-G C-G A-T T-A T-A C-G G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G G-A-A-U-G-G-C-C -U Anti-Codon (tRNA) Codon (mRNA) Glutamic Acid (AA) A-T G-C G-C C-G C-G A-T T-A T-A C-G G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Trp ACC G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Trp ACC G-A-A-U-G-G-C-C -U Anti-Codon (tRNA) Codon (mRNA) Tryptophan (AA) A-T G-C G-C C-G C-G A-T T-A T-A C-G Trp ACC G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Trp ACC G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Trp ACC G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Pro GGA G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Pro GGA G-A-A-U-G-G-C-C -U Anti-Codon (tRNA) Codon (mRNA) Proline (AA) A-T G-C G-C C-G C-G A-T T-A T-A C-G Pro GGA G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Pro GGA G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Pro GGA G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Pro G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Pro GGA G-A-A-U-G-G-C-C -U A-T G-C G-C C-G C-G A-T T-A T-A C-G Pro GGA G-A-A-U-G-G-C-C -U Codons that translate for specific Amino Acids (or Start and Stop codes) Translation is done. Now… Questions to answer… 1) What is the end result of Translation? 2) Where does Translation take place in the cell? 3) During which part of the cell cycle does Translation take place? 4) What is the monomer of proteins? 5) Where are excess protein monomers found in the cell? 6) How are the protein monomers bonded to each other? 7) How many different amino acids do humans need to make proteins? 8) How many of the amino acids are “essential”… we can’t make them, we must get them in our diet? 9) What is a “codon”? 10) What is an “anti-codon”? 11) Where does mRNA come from and what’s its function? 12) Where does tRNA come from and what’s its function? Done