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Ecological Parasitology Lab 5: Malaria parasites Classification: Domain: Eukarya Kingdom: Kingdom: Chromalveolata (descendents of endosymbiosis of red algae and bi-flagellate) Phylum: Apicomplexa Class: Haemosporidia 3 main genera: Plasmodium, Leucocytozoon, Haemoproteus Life cycle stages: Sporozoites: infective stage, passed from vector to vertebrate host Trophozoite: feeding stage, in cells of vertebrate host Schizont: multi-nucleated stage, asexual replication, in cells of vertebrate host Merozoite: produced from schizont when it divides, one nucleus, invades new vertebrate host cells, develops into trophozoite Gametocyte: sexual cell, male and female, passed from vertebrate host to vector, produces gametes in vector which combine to form zygote Ookinete: mobile stage, penetrates the midgut wall of vector Oocyst: on midgut wall of vector, asexual replication occurs within it, produces thousands of sporozoites Today’s Parasites Species Notes Plasmodium falciparum See back P. malariae See back P. vivax See back P. azurophilium Parasite of Carribean lizards, infects red and white blood cells Parasite of African lizards, huge gametocytes and schizonts Parasites of birds, vectored by black flies and midges, schizogony in liver Parasites of birds, vectored by Hippoboscid fly, schizogony in tissues P. giganteum Leucocytozoon Haemoproteus Label Troph, gametocyte, RBC Troph, schizont, gametocyte, RBC, WBC Describe thick smear, label gametocyte, trophozoite, RBC Life cycle stage of all parasites visible, RBC, WBC Gametocyte, schizont, RBC Gametocyte, normal RBC, infected RBC Gametocyte, RBC