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TB20 Basic Definitions BACTERIA: Any of a group of diverse, ubiquitous, microscopic single-celled microorganisms. SPORE: The inactive/dormant, protected/resistant form that some bacteria can temporarily assume, when conditions are not satisfactory for active metabolism and cell reproduction. ENZYME: (a/k/a non-living chemical catalyst) Any of various complex organic substances originating from living microorganisms, and capable of producing certain chemical changes in organic substances by catalytic action. Enzymes are the chemical catalysts of living cells. NOTE : While bacteria metabolize a wide variety of organic material, enzymes are substrate specific. For example: vP vProtease enzyme catabolizes (“breaks down”) protein v Amylase enzyme breaks down starch and carbohydrate v Lipase enzyme breaks down fat and grease v Xylanase enzyme breaks down plant material vC vCellulase enzyme breaks down cellulose v Urease enzyme breaks down urea AEROBIC BACTERIA: Bacteria that require the presence of oxygen to live and function. ANAEROBIC BACTERIA: Bacteria that do not require the presence of oxygen to survive—they are capable of living and functioning in the absence of oxygen. FACULTATIVE BACTERIA: Bacteria that are capable of living and functioningeither in the presence of oxygen, or in the absence of oxygen. COLONY FORMING UNIT (CFU): The standard microbiological method used to count bacteria. The number of viable cells that give rise to a colony of bacteria on a suitable agar medium. 1048 Stinson Drive, Reading PA 19605 Phone: 1-800-548-4568 www.miscoproducts.com May 2013 Page 2 - TB20 Growth PH range Temperature (ºC) Protease (breaks down protein) Amylase (breaks down starch & carbohydrates) Lipase (breaks down fat & grease) Esterase (breaks down fat) Cellulase (breaks down cellulose) Xylanase (breaks down plant material) Urease (breaks down urea) Basic Definitions BacillusB sBacillusB amyloliquefaciens Faculatitive* 6 - 10 10 - 50 linchenformis Faculatitive*A 5 - 10 10 - 50 sBacillusB subitilism *AerobicA 4 - 10 10 - 50 sBacillusB egaterium**p cAerobic 5 - 10 4 - 45 sBacillus *pumilus Aerobic 6 - 10 10 - 50 ++ ++ ++++ +++ +++ ++ ++ ++++ + + ++ ++ ++++ +++ +++ ++ ++ ++++ +++ +++ ++ + ++++ +++ ++++ ++ + ++++ +++ ++++ +++ ++ ++ ++++ ++ ++++= Excellent +++ = Very Good ++ = Good + = Moderate * Faculative: capable of living and functioningeither in the presence of oxygen (aerobic) or in the absence of oxygen (anaerobic). ** Bacillus megaterium, under laboratory conditions, germinates in one hour or less. MostBacillus strains require a minimum of four hours to germinate. 1048 Stinson Drive, Reading PA 19605 Phone: 1-800-548-4568 www.miscoproducts.com May 2013