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Nephrology Dialysis Transplantation 28 (Supplement 1): i322–i324, 2013 doi:10.1093/ndt/gft127 HORMONES MP074 ENDOGENOUS OUABAIN AND BLOOD PRESSURE VARIABILITY EVOKED BY LOW SALT INTAKE IN ESSENTIAL HYPERTENSION Guido Gatti1, Chiara Lanzani1, Elisabetta Messaggio1, Nunzia Casamassima1, John Hamlyn2, Marco Simonini1 and Paolo Manunta1 1 OU Nephrology and Dialysis / Chair of Nephrology San Raffaele Scientific Institute / Università Vita Salute San Raffaele Milan Italy, 2Physiology Department University of Maryland, School of Medicine Baltimore MD United States Introduction and Aims: A series of experimental and clinical data supports the notion that Endogenous Ouabain (EO) may affect blood pressure (BP), plasma sodium concentration, and renal Na excretion. The plasma sodium concentration directly affects BP but the pressor mechanism for elevated BP following an increased salt intake is unclear. A direct relation between plasma EO and plasma Na has been reported. Further, the BP response to a low salt intake is heterogeneous, with BP rising in some patients. Methods: Here we investigated the response of EO, plasma Na and BP to a low salt intake (<100 mEq/day, 15 days) in a cohort of 79 naïve hypertensive patients (EH). Results: The BP response to the low salt intake showed high systolic and diastolic BP variability (range -62.4 to +40 mmHg, respectively). The changes in mean diastolic BP were directly related to the change in plasma EO (r=0.27, p=0.02). Patients were then segregated into quartile sub-groups according to DBP changes. Those with the greatest BP fall were in the 1st quartile, while those patients that showed an increase in DBP after low salt intake, i.e., reverse salt sensitivity (RSS), were in the 4th quartile (Figure A panel). RSS showed a greater decrease in plasma [Na+] (-2.2±0.6 vs + 0,23±0.54 mEq/ L, B panel) and simultaneous increases in plasma EO (C panel). Circulating EO was inversely related to plasma [Na+] (r=-0.316, p=0.005, n=78). Neither plasma aldosterone nor renin were related to RSS. Conclusions: Our findings show that in EH: 1) a low salt diet lowers BP in the vast majority of patients while raising plasma EO and BP primarily in RSS patients. 2) While EO upregulates renal Na-K ATPase activity,and might be a useful mechanism minimizing sodium loss, the elevation of EO in RSS patients suggests it may account for the paradox that reduced salt intake may increase the risk for cardiovascular and renal disease in some individuals. Introduction and Aims: Adiponectin (ADPN) is an adipokine with anti-atherogenic, anti-inflammatory and insulin-sensitizing properties. To date is reported that the adipocyte is the predominant cell type responsible for secretion of ADPN and that the chronic inflammatory status characterizing obese patients is responsible for a reduction of ADPN circulating levels. In vivo studies demonstrated that the hypoadiponectinemia induces microalbuminuria through a direct podocytes dysfunction. However clinical studies demonstrated that patients with overt proteinuria had higher circulating ADPN levels compared to normoalbuminuric controls. Unexpectedly, similar ADPN levels were observed in obese proteinuric patients. To date, despite all these evidences, the mechanisms linking overt proteinuria and hyperadiponectinemia are not yet clarified. The aim of our study was to investigate whether epithelial tubular renal cells express and secrete adiponectin and, principally, whether renal cells in basal conditions and upon an inflammatory stimulus secrete this adipokine contributing to ADPN circulating levels. Methods: In human proximal tubular epithelial cells, HK-2, ADPN mRNA was evaluated by real time PCR-assay while protein expression levels by Western blot analysis and immunofluorescence assay. Moreover, renal ADPN distribution was assessed by immunohistochemical analysis of kidney biopsies from healthy subject and from two patients affected by rapidly progressive and membranous glomerulonephrytis respectively. Finally, by ELISA assay, we measured ADPN concentrations in culture media of HK-2 cells treated with lipopolysaccharide (LPS) 10 µg/ml. Results: Our analyses revealed that HK-2 cells express ADPN both in terms of mRNA and protein. These results were confirmed by the observed cytoplasmatic HK-2 intense immunoreactivity for ADPN antibody and by immunohistochemical analysis showing a diffuse ADPN distribution in normal kidney tissue. We also confirmed that HK-2 cells express both best-characterized receptors for ADPN, adipoR1 and adipoR2 although, the results revealed that adipoR1 is the predominant isoform. Furthermore we observed that tubular cells secrete ADPN in basal condition and, more interestingly, this secretion significantly increases ( p < 0.05) upon LPS treatment in a time dependent manner. Finally, immunohistochemical analysis of kidney biopsies obtained from patients affected by membranous and rapidly progressive glomerulonephritis showed a similar pattern of ADPN staining observed in healthy control. Conclusions: Our study demonstrates, for the first time, that tubular renal cells express and secretes ADPN, which concentration increases upon inflammatory stimulus. These results suggest that in renal inflammatory diseases, tubular cells may contribute to the increasing ADPN circulating levels, triggering a feedback response in order to self-mitigate the inflammatory process. MP076 ANTIDIURETIC EFFECTIVENESS OF DESMOPRESSIN ENCAPSULATED IN NEWLY DESIGNED LIPOSOMAL CARRIERS Olga Gawrys1, Katarzyna Gawarecka2, Ewa Swiezewska2, Marek Masnyk3, Marek Chmielewski3 and Elzbieta Kompanowska-Jezierska1 1 Mossakowski Medical Research Centre Polish Academy of Sciences Warsaw Poland, 2Institute of Biochemistry and Biophysics Polish Academy of Sciences Warsaw Poland, 3Institute of Organic Chemistry Polish Academy of Sciences Warsaw Poland MP074 MP075 ADIPONECTIN IS EXPRESSED AND SECRETED BY TUBULAR EPITHELIAL RENAL CELLS Anna Perri1, Donatella Vizza1, Danilo Lofaro1, Paolo Gigliotti1, Francesca Leone1, Teresa Papalia1 and Renzo Bonofiglio1 1 Dept of Nephrology, Annunziata Hospital “Kidney and Transplantation” Research Center Cosenza Italy Introduction and Aims: Recently liposomal formulation lies within the interest of many researchers. Drug carriers based on liposomes are considered to be efficient and convenient enabling pharmacologically active substances better penetration through biological membranes. Augmenting carrier properties by using new components seems to be a very profitable strategy to improve therapy. Previous studies on amino-prenols, new derivatives of polyisoprenoid alcohols, proved their lipofecting properties and showed their lack of toxicity in rats. In this study we investigate how they affect the bioavailability of desmopressin (dDAVP). This agonist of vasopressin acts selectively on V2receptors, responsible for the antidiuretic effect and is deprived of its vasoconstrictory action. Antidiuretic effectiveness of dDAVP encapsulated in new liposomes built of dioleoyl phosphatidylethanolamine and amino-prenols (dDAVP +LP, 60ng/kg of dDAVP in 7.5 mg/kg of lipids) was compared to dDAVP (60ng/kg) given in classical solvent (water).Two control groups received: liposomes without dDAVP (LP,7.5 mg/kg of lipids) and solvent for classical dDAVP (W). Methods: In acute experiments anaesthetized and surgically prepared male Sprague-Dawley rats received intravenous infusion of 2.5% glucose solution (36 ml/h/ kg) to induce water diuresis. After 30 minutes the corresponding solution (dDAVP+LP, dDAVP, LP or W) was given intravenously as a bolus (0.5 ml for 1 min). In the clearance experiment diuresis (V), plasma osmolality and solute excretion were measured. Results: Different kinetics of inhibition of diuresis caused by dDAVP encapsulated in liposomes and for dDAVP given in water was observed. After administration of dDAVP in water decrease in diuresis was visible after 0.5 h, however after 1.5h diuresis slightly started to increase again. dDAVP+LP inhibited diuresis after 1h and after 2h the decrease was still observed. This was probably caused by slower release of © The Author 2013. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please e-mail: [email protected] Abstracts Nephrology Dialysis Transplantation desmopressin from liposomal carriers; in this case dDAVP+LP lasted longer in cardiovascular system and was characterized by prolonged activity. In the end of observation diuresis (dDAVP: 33.1±7.5 µl/min; dDAVP+LP: 38.1±8.7 µl/min) and plasma osmolality (dDAVP: 266±2; dDAVP+LP: 263±2 mosm/l) in both groups was similar. Also we did not observe any negative changes in cardiovascular system caused by intravenous administration of amino-prenols, so we obtained additional confirmation that they are not toxic for living organisms. Conclusions: Slower release of dDAVP+LP from liposomal carriers into systemic circulation caused the delay in its antidiuretic response, but the final effectiveness of the two routes of drug application was comparable. In addition, an inducement of prolonged activity of applied drugs by our liposomes could be beneficial. MP077 LOW TRIIODOTHYRONINE SYNDROME IN DIALYSIS: DOES THE VASCULAR ACCESS PLAYS ANY ROLE? Francesca Apponi1, Valentina Sinibaldi1, Anna Giuliani1, Matteo Baldinelli2, Remo Luciani3, Franco Giordano4, Giancarlo Panzieri4, Giorgio Punzo1, Paolo Mené1 and Nicola Pirozzi1 1 Department of Clinical and Molecular Medicine, UOC Nephrology and Dialysis, AO Sant'Andrea University of Rome Sapienza Rome Italy, 2Department of Clinical Science University of Rome, Sapienza Rome Italy, 3UOC Nefrologia AO G. Rummo Benevento Italy, 4Nephrology Department Casa di Cura Nuova ITOR Rome Italy Introduction and Aims: Low triiodothyronine (fT3) syndrome has been reported with a high prevalence in chronic kidney disease (CKD) and have been considered an independent predictor of mortality in both hemodialysis (HD) and peritoneal dialysis (PD) patients. Several factors, such as malnutrition, inflammation, acute disease, are reported to cause this syndrome in ESRD patients. Our aim was to evaluate whether the incidence of low T3 syndrome in a dialysis patients group was influenced by the vascular access in use: autogenous arteriovenous fistula (AVF) compared to arteriovenous graft and central venous catheter (AVG -CVC). Methods: We studied 43 stable chronic hemodialysis patients (mean age, 60 years; M 30, F 13), divided in group A (AVF, n=21) and group B (AVG-CVC, n=22). The following parameters were measured in every patient: TSH, fT4, fT3, biochemical data related to nutritional status (serum albumin) and markers of inflammation (C-reactive protein, VES, fibrinogen). We excluded from this study any patients with intercurrent illnesses (infectious diseases, cancer, and hospitalization within the previous 60 days) and those treated with drugs known to affect the plasma concentration of thyroid hormones (beta-blockers, amiodarone, levothyroxine). Results: Group B (AVG-CVC) showed lower mean value of fT3 (1,91 pg/ml ± 0.56) compared to Group A (2,34 pg/ml ± 0.31) ( p<0.004). No significant difference was observed between AVG and CVC patients in group B. TSH did not differ between two groups (Group A: 1,19 uIU/ml ± 0.66, Group B: 1,29 uIU/ml ± 1.11), whereas Group B showed higher fT4 (0,91 ng/dl ± 0.28) compared to Group A (0,74 ng/dl ± 0.12) ( p< 0.008). C-reactive protein (CRP) and fibrinogen were higher in Group B (CRP 5,84 mg/dl ± 8.6, fibrinogen 391 mg/dl ± 111) then Group A (CRP 0,70 mg/dl ± 0.53, fibrinogen 302 mg/dl ± 61.4) ( p< 0.004 and p< 0.003 respectively), but no significant correlation was found with fT3 levels. Nutritional status (serum albumin) did not differ between groups, nor any correlation with fT3 levels was found. Conclusions: Our results would suggest that type of vascular access could influence the incidence of lowT3 syndrome in stable hemodialysis patients, independently from inflammation/nutritional parameters. Autogenous accesses (AVF) seems protective, by a mechanism that need to be clarified. MP078 HEMODIALYSIS-INDUCED CHANGES IN THYROID HORMONE CONCENTRATIONS Sylvie Dusilova Sulkova1, Jiri Horacek2, Roman Safranek1, Marta Kalousova4, Eva Malirova3, Blanka Dlabalova3, Michaela Kubisova1 and Pavel Zak2 1 Hemodialysis Center Medical Faculty and University Hospital Hradec Kralove Czech Republic, 2IV. Department of Internal Medicine Medical Faculty and University Hospital Hradec Kralove Czech Republic, 3Department of Nuclear Medicine Medical Faculty and University Hospital Hradec Kralove Czech Republic, 4 Institute for Clinical Biochemistry Charles University Prague Czech Republic Introduction and Aims: Thyroid function tests are often abnormal in end-stage renal disease patients on maintenance hemodialysis. However, the effect of single hemodialysis (HD) procedure on thyroid hormone serum concentrations is not known. Methods: Hemodialysis patients (n=110, 71 M, median age 65 years) were studied during conventional low-flux HD procedure (spKt/V 1.52; 1.32-1.72). Pre- and post-HD serum concentrations of total and free thyroxine (T4 and fT4), total and free triiodothyronine (T3 and fT3), reverse triiodothyronine (rT3) and thyrotropin (TSH) levels were measured using radioisotope assays. Data are given as median; interquartil range. Results: All thyroid hormones increased significantly during HD (P<0.001, Wilcoxon test): T4 by 18.1%, fT4 by 45.5%, T3 by 14.6%, fT3 by 8.7%, and rT3 by 15.7%. The ratio free/total T3 did not change during HD. On the contrary, the ratio free/total T4 increased significantly from 1.658-4 to 1.973-4 (P<0.0001). Conversely to peripheral Volume 28 | Supplement 1 | May 2013 thyroid hormones, TSH levels significantly decreased during HD from 1.80 to 1.60 mIU/l. Data are shown in the table. Conclusions: HD procedure induces profound changes in serum thyroid hormones concentrations. The concordant increase in all thyroid peripheral hormones (maximum in fT4) suggests an increased release of the hormones from thyroid gland into peripheral circulation and is probably responsible for observed decrease in TSH. We can only hypothesize that removal of an excess of iodide during HD might be responsible for observed changes (Wolff-Chaikoff effect). Removal of other non-specific uremic toxins may also be involved, as well as correction of metabolic acidosis. Finally, the timing of blood sampling (before vs. after HD) is important for proper interpretation of thyroid function tests in HD patients. MP078 TSH (mIU/L) fT3 (pmol/L) T3 (nmol/l) fT4 (pmol/L) T4 (nmol/L) rT3 (nmol/L) MP079 Pre-HD 1.80; 1.21-3.47 3.39; 2.91-3.78 0.98; 0.81-1.19 12.07; 10.50-14.12 70.9; 59.6-83.4 0.265; 0.211-0.363 Post-HD 1.60; 0.90-2.73 3.61; 3.12-4.36 1.12; 0.91-1.33 17.87; 15.91-20.62 91.1; 74.0-108.6 0.311; 0.242-0.430 P< 0.001 0.001 0.001 0.001 0.001 0.001 PROLACTIN EFFECT ON WATER-SOLUTE BALANCE IN THE RAT MODEL OF CHOLESTASIS OF PREGNANCY IS RENAL AQUAPORIN INDEPENDENT Natalia Sirotina1, Yuri Fidchenko1 and Olga Smirnova1 1 Laboratory of Endocrinology, Biological Department Lomonosov Moscow State University Moscow Russian Federation Introduction and Aims: Prolactin regulates lactation in mammals, but in fishes and lower vertebrates it controls water-solute balance. Level of this hormone elevates during pregnancy and additionally elevates under condition of cholestasis of pregnancy. Our goal was to determine whether prolactin has any effect on water-solute homeostasis in female rats in the model of cholestasis of pregnancy. Methods: For the modeling of pregnancy's prolactin level hyperprolactinemia was induced by donor pituitary transplantation under renal capsule of female rat recipient, for the modeling of cholestasis of pregnancy the combination of induced hyperprolactinemia and bile duct ligation was used. Hyperprolactinemia was confirmed by measure of rat serum prolactin concentration. Surgical procedures were conducted under diethyl ether anesthesia. In these models diurnal diuresis, glomerular filtration rate (GFR) and diurnal sodium excretion were estimated. Aquaporin 1–4 mRNA expression in the renal inner medulla was tested by real-time PCR using 3 housekeeping genes and normalized on GAPDH expression. Results: Persistent hyperprolactinemia combined with obstructive cholestasis led to sharp 2-fold elevation of diurnal diuresis and compensatory water consumption as compared with the control. In spite of this aquaporin 1–4 mRNA expression in the renal inner medulla and glomerular filtration rate were not changed in this model of cholestasis of pregnancy. In this model sufficient elevation of diurnal sodium excretion as compared with control groups was revealed. Alone bile duct obstruction or hyperprolactinemia had no marked influence on these parameters. Conclusions: The data on diuretic and natriuretic effects of prolactin in the model of cholestasis of pregnancy together with the lack of prolactin influence on GFR and aquaporin 1-4 mRNA expression in renal medulla let us to suggest primary prolactin influence on the sodium transporters in the kidney without substantial modulation of aquaporin expression and vasopressin action. Supported by RFBR (grant 11-04-00009-a). MP080 EFFECTS OF A RESISTANCE EXERCISE TRAINING PROGRAM ON ACYL- GHRELIN AND OBESTATIN LEVELS IN HEMODIALYSIS PATIENTS Cristiane Ferreira Moraes2, Sandra Mara Marinho1, Milena Barcza Stockler-Pinto3, Amanda Faria Barros2 and Denise Mafra1,2 1 Graduate Program in Medical Sciences Federal University Fluminense Niteroi Rio de Janeiro Brazil, 2Graduate Program in Cardiovasculare Sciences Federal University Fluminense Niteroi Rio de Janeiro Brazil, 3Institute of Biophysics Carlos Chagas Filho Federal University of Rio de Janeiro Rio de Janeiro Rio de Janeiro Brazil Introduction and Aims: Appetite hormones peptides are altered by exercises in general population; however, no study has evaluated the effects of exercise on these hormones in chronic kidney disease (CKD) patients. The purpose of this study was to assess the effects of an intradialytic resistance exercise training program (RETP) on plasma levels of gut peptides (acyl-ghrelin and obestatin) in hemodialysis (HD) patients. doi:10.1093/ndt/gft127 | i Abstracts Nephrology Dialysis Transplantation Methods: The study enrolled 37 hemodialysis (HD) patients (61.5% men, 45.9±14.1 yrs, 23.5±3.9kg/m2).Acyl- ghrelin and obestatin plasma levels (measured using the enzyme immunometric assay) were performed in the fasted state at baseline and after 6 months of RETP (supervised, 3 days/wk, total 72 sessions). Anthropometric measurement and food intake were assessed. Statistical analyses were performed using SPSS 17.0. Results: After 6 months RETP, there was increase in men fat free mass (from 51.3±10 to 53.2±10kg, p<0.05) and arm muscle area in all patients. The energy and protein intakes were similar before and after exercises, however; there were significant reduction in the anorexigenic hormone levels (obestatin) from 3.0 (2.3-3.4) ng/mL to 1.9 (0.6-3.4ng/mL) and increase in the orexigenic (acyl-ghrelin) from 21.5 (1.3-77.7) ng/mL to 37.2 (16.7-94.1) ng/mL . Conclusions: In conclusion, the resistance exercise during 6 months in HD patients led MP080 Parameters Albumin (mg/dL) Creatinine (mg/dL) Kt/V Potassium (mg/dL) Acyl-ghrelin (pg/mL) Obestatin (ng/mL) Acyl-ghrelin/Obestatin ratio Before 3.7±0.3 12.4±3.5 1.4±0.3 4.9±0.5 21.5 (1.3-77.7) 3.0 (2.3-3.4) 0.07 (0.01-0.06) After 3.9±0.2* 12.3±3.3 1.3±0.4 4.8±0.6 37.2 (16.7-94.1)* 1.9 (0.6-3.4)* 0.2 (0.08-0.7) Introduction and Aims: Irisin, a recently discovered hormone secreted by myocytes induced in exercise, acts as a muscle-derived energy-expenditure signal that binds to undetermined receptors on the white adipose tissue surface, stimulating its browning and uncoupling protein 1 (UCP1) expression. The purposes of this study were to assess the effect of an intradialytic resistance exercise training program (ETP) on circulating concentrations of irisin in hemodialysis (HD) patients and compare irisin plasma levels in these patients and healthy individuals. Methods: This longitudinal study enrolled 26 chronic kidney disease patients (CKD) (50% men, 44.7±14.1 yrs, 23.5±3.9kg/m2). The healthy individuals group consisted of 11 women and 7 men with mean age of 50.9 ± 6.1 yrs and BMI, 24.2 ± 2.7kg/m2. Anthropometric and biochemistry parameters (irisin by Enzyme-Linked Immunosorbent Assay) were measured at the baseline and after 6 months of strength ETP (in both lower limbs) that was performed during the first 3 hours of hemodialysis, three times a week for 72 sessions. Results: There was no difference regarding gender, age and body mass index (BMI) between HD patients and healthy individuals. Baseline plasma levels of irisin in HD patients were significantly lower than in healthy individuals (71.0±41.6 vs 101.3 ± 12.5ng/mL, p<0.05). There was no significant difference in irisin plasma levels between women and men (77.5 ± 44.4 vs 64.6 ± 39.4 ng/mL). Although the muscle mass has increased in consequence of exercise (evaluated by arm muscle area from 27.9 (24.1) to 33.1(19.0)cm2), irisin plasma levels did not differ significantly before and after ETP (71.0 ± 41.6 vs 73.3 ± 36.0 ng/mL). Conclusions: HD patients seem to have lower irisin levels when compared to healthy individuals. Moreover, a resistance exercise training program was unable to augment irisin levels despite increasing muscle mass. MP083 to significant changes in appetite hormones and it seems a so good intervention to modulate appetite in HD patients. MP081 SERUM DEHYDROEPIANDROSTERONE SULFATE IS ASSOCIATED WITH SKELETAL MUSCLE MASS, ARTERIAL STIFFNESS, AND DEPRESSIVE MOOD IN JAPANESE MALE HEMODIALYSIS PATIENTS Makoto Inoue2, Takako Saito1, Koushi Ueno2, Akio Yoshimura2, Hisashi Yamamoto1, Osamu Saito1 and Eiji Kusano1 1 Nephrology JIchi Medical University Shimotsuke, Tochigi Japan, 2Dialysis Unit Kawashima Medical Clinic Chikusei, Ibaraki Japan Introduction and Aims: The dialysis population is aging. There is a clear age-related declined in dehydroepiandrosterone sulfate (DHEAs) and this has suggested that a relative deficiency in this steroid may be causally related to the development of a series of diseases associated with aging including cardiovascular diseases, osteoporosis, and depressive mood. The aim of this study was to examine the effect of serum DHEAs on body composition and aging-associated diseases in hemodialysis patients. Methods: Cross sectional observational study comprising 61 hemodialysis patients (39 men, mean age 65.2±1.2 years). Serum DHEAs levels were measured by radioimmunoassay. The Inbody S20 Body Composition Analyzer was used for bioelectrical impedance analysis. Brachial-ankle pulse wave velocity (baPWV) and quantitative ultrasound of the calcaneus were measured. Depressive symptoms were ascertained with the Patient Health Questionnaire (PHQ-9). Results: The mean DHEAs levels of the male versus the female were 1059 versus 739 ng/mL. DHEAs correlated negatively with age ( p<0.001) and baPWV ( p<0.005), and positively with skeletal muscle mass ( p<0.01) in men, but not in women. In patients with higher scores on the PHQ-9, the levels of DHEAs were significantly lower (640 ng/mL) than the levels in other patients (1197 ng/mL). There were no significant associations between DHEAs and the values of calcaneal speed of sound, which is correlated with bone density. Conclusions: In male dialysis patients, the lower levels of DHEAs were associated with aging process, such as decreased skeletal muscle mass, increased arterial stiffness, and depressive mood. Large prospective trials and intervention studies are needed to better assess these benefits of DHEAs in male dialysis patients. MP082 EXERCISE TRAINING DOES NOT AFFECT THE PLASMA IRISIN CONCENTRATION IN HEMODIALYSIS PATIENTS Cristiane Ferreira Moraes1, Viviane Oliveira Leal3, Sandra Mara Marinho2, Sergio Girão Barroso3, Gabrielle S. Rocha3, Gilson Teles Boaventura2 and Denise Mafra1,2 1 Graduate Program in Cardiovasculare Sciences Federal University Fluminense Niteroi Rio de Janeiro Brazil, 2Graduate Program in Medical Sciences Federal University Fluminense Niteroi Rio de Janeiro Brazil, 3Clinical Nutrition Federal University Fluminense Niteroi Rio de Janeiro Brazil i | Abstracts ENDOGENOUS ANABOLIC HORMONES AND HIGH LOAD STRENGTH TRAINING IN MALE PATIENTS UNDERGOING DIALYSIS Stig Molsted1, Jesper L. Andersen2, Inge Eidemak3, Adrian Harrison4 and Niels Jørgensen5 1 Department of Cardiology, Nephrology & Endocrinology Hillerød University Hospital Hillerød Denmark, 2Institute of Sports Medicine Copenhagen Bispebjerg University Hospital Copenhagen Denmark, 3Department of Nephrology P Copenhagen University Hospital, Rigshospitalet Copenhagen Denmark, 4 Department of Basic Animal and Veterinary Sciences Faculty of Health and Medical Sciences, Copenhagen University Copenhagen Denmark, 5University Department of Growth and Reproduction Copenhagen University Hospital, Rigshospitalet Copenhagen Denmark Introduction and Aims: Sufficient anabolic hormone levels and functions are important to avoid muscle atrophy and to induce muscle hypertrophy in relation with resistance training. In male patients undergoing dialysis testosterone levels are in general lower than in healthy persons. IGF-1 expressions may also be impaired. The aim of this study was to investigate circulating testosterone and IGF-1 in a controlled design before and after 16 weeks of high load strength training in male patients undergoing dialysis and to investigate if hormone plasma levels were associated with muscle morphology. Methods: Thirteen male patients aged 56 ± 4 (mean ± SEM) years were tested before and after a 16 weeks control period and before and after 16 weeks of high load resistance training thrice weekly. The training comprised leg press, knee extension, and knee flexion. Samples of plasma were obtained after an over-night fast to analyse testosterone, IGF-1, and LH. Hormone values from the male general population was delivered by the University Department of Growth and Reproduction, Rigshospitalet, Copenhagen University Hospital, Denmark. Muscle fibre size and percentage number were analysed in muscle biopsies from the vastus lateralis muscle. Results: Total testosteron (24.6 ± 3.4 nmol/l), free testosterone (520 ± 73 pmol/l), IGF-1 (262.8 ± 38.5 ng/ml), and IGF-BP3 (5452 ± 485 ng/ml) remained stable during the control and training period and was within normal ranges. All participants had LH values above normal age matched mean values. Age adjusted testosterone was not associated with muscle fibre size at pre test. Age adjusted log IGF-1 was significantly associated with type 1 fibre size (3944 (1170-6717) coefficient (95% CI), p = 0.008) and type 2 fibre size (2348 (586-4110), p = 0.012) at pres test. During the training period total testosterone variation was positively associated with type 2 muscle fibre size variation (r = 0.664, p = 0.026), and negatively associated with type 2 muscle fibre area variation (r = 0.664, p = 0.012). Conclusions: Circulating testosterone and IGF-1 was not changed by 16 weeks resistance training. Mean testosterone values were normal due to markedly increased LH values, which suggest testes dysfunction. Muscle size depend on IGF-1 levels. Variations within total testosterone may be associated with type 2 muscle fibre size and architecture variations in relation with resistance training in male patients undergoing dialysis. Volume 28 | Supplement 1 | May 2013