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Pacifici E. CURRICULUM VITAE ET STUDIORUM Personal data Name: Elena Pacifici Education 2015 University of Rome “Sapienza”, Italy. PhD studentship in Genetics and Molecular Biology. 2011 University of Rome “Sapienza”, Italy. Master degree in Genetics and Molecular Biology. Final mark: 110 /110 cum laude. 2009 University of Rome “Sapienza”, Italy. Bachelor’s degree in Biological Sciences, curriculum in Genetics and Molecular Biology. Final mark: 100/110. Research Experience 2015-present: Research work as PostDoc in the laboratory of Prof.ssa Sabrina Sabatini Dept. of Biol. and Biotec. “C. Darwin”-“Sapienza” University of Rome, Italy. Research project: "Meccanismi molecolari coinvolti nella formazione di confini cellulari nella radice di Arabidopsis". Main focus of the work: "To understand which molecules are necessary to regulate the correlation between cell size and cell fate in the Arabidopsis root". 2011 - 2014: PhD Research training in the laboratory of Prof.ssa Sabrina Sabatini Dept. of Biol. and Biotec. “C. Darwin”-“Sapienza” University of Rome, Italy. Title of PhD project thesis: "Molecular mechanisms of interaction between cellular expansion and cytokinin signaling". Main focus of the work: To understand which molecules are necessary to regulate the correlation between cell size and cell division and/or differentiation input. 2009 - 2011: Research training in the laboratory of Dott.ssa Sabrina Sabatini, Dept. of Biol. and Biotec. “C. Darwin”-“Sapienza” University of Rome Italy. Final dissertation: “Molecular mechanisms depending on the interaction between cytokinin and auxin necessary to the maintenance of the Arabidopsis thaliana root stem cell niche. Pacifici E. Main focus of the work: to investigate the molecular mechanisms controlling the stem cell niche differentiation in the scr1-1 mutant after the ARR1 ectopic expression in the root stem cell niche and to understand if ARR1 activates the same molecular circuit acting at the transition zone . 2008 - 2009: Undergraduate training in the Lab of Dott.ssa Sabrina Sabatini, Dept. of Biol. and Biotec. “C. Darwin”-“Sapienza” University of Rome, Italy. Final dissertation: Role of the cytokinin hormone in the maintenance of the Arabidopsis thaliana root stem cell niche. Main focus of the work: to determinate the relationship between SCR and ARR1 genes and if the loss of function mutant of ARR1 gene complements the scr1-1 mutation. Fellowship and Awards 2011: Fellowship for excellence in academic performance of PhD School in Genetics and Molecular Biology - XXVII cycle - “Sapienza” University of Rome, Italy. Research results including publications, teaching and meetings Publications Moubayidin L, Di Mambro R, Sozzani R, Pacifici E, Salvi E, Terpstra I, Bao D, van Dijken A, DelloIoio R, Perilli S, Ljung K, Benfey PN, Heidstra R, Costantino P, Sabatini S. (2013) Spatial coordination between stem cell activity and cell differentiation in the root meristem. Developmental Cell. 26, 405-15. Pacifici E, Polverari L, Sabatini S. (2015) Plant hormones crosstalk: the pivot of root growth. Journal of Experimental Botany, doi:10.1093/jxb/eru534. Teaching 2015 “Genome editing: the CRISPR/Cas9 technology” for the course of Molecular Biology of Model System Development. Tutor: Prof.ssa Sabrina Sabatini, University of Rome “Sapienza”. 2015 “The plant cell wall” for the course of Molecular Biology of Model System Development. Tutor: Prof.ssa Sabrina Sabatini, University of Rome “Sapienza”. 2013 “The plant cell wall” for the course of Molecular Biology of Plant Development. Tutor: Prof.ssa Sabrina Sabatini, University of Rome “Sapienza”. 2012 “The plant cell wall of Arabidopsis thaliana model system” for the course of Molecular Biology of Plant Development. Tutor: Prof.ssa Sabrina Sabatini, University of Rome “Sapienza”. Pacifici E. Meetings and Submitted Posters 2014- The International Symposium on Auxins and Cytokinins in Plant Development ACPD 2014Prague, Czech Republic. PosterI: Elena Pacifici, R. Di Mambro, R. Sozzani, P. Benfey, W. Busch, P. Costantino, and S. Sabatini. (2014) Molecular mechanisms of interaction between cellular expansion and cytokinin signaling. PosterII: Di Mambro R, De Ruvo M, Perilli S, Sozzani R, Pacifici E, Benfey P, Busch W, Costantino P and Sabatini S. (2014) Tissue specific cross-talk between cytokinins and auxin is critical for Arabidopsis root meristem growth and development. 2012- 2012 EMBO Conference Series Plant Development and Environmental interactions Matera, Italy PosterI: Elena Pacifici, P. Costantino, and S. Sabatini. (2012) Molecular mechanisms of interaction between cellular expansion and cytokinin signaling. Poster II: Laila Moubayidin, R. Di Mambro, E. Pacifici, I. Terpstra, S. Perilli, R. Dello Ioio, R. Heidstra, P. Costantino, and S. Sabatini. (2012) SCARECROW sustains stem cell activity inhibiting cytokinin dependent cell differentiation input. Data processing knowledge Proficient in Microsoft Office, including Word, Power Point and Excel. Proficient in ImageJ, Adobe Illustrator and Photoshop CS2, CS5 and CS6. Proficient in online database Arabidopsis eFP Browser, The Arabidopsis Information Resource (TAIR), Salk Institute Genomic Analysis Institute (SiGnAL), The European Arabidopsis Stock Centre (uNASC). Proficient in Confocal microscopy, trained with Zeiss LSM 780. Proficient in Real Time PCR, trained with Applied Biosystems 7500 FAST Real Time PCR. Participation at workshop: Course on “Real Time and Applications”- 28.07.2014 - Dept. Biology and Biotechnology, Via dei Sardi, 70-Rome, Italy. Dr.ssa Serena Grimaldi-qPCR/CE Sr Associated Sales FAS Life Technologies Brand. Date 16.12.2015 Elena Pacifici