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Pacifici E.
CURRICULUM VITAE ET STUDIORUM
Personal data
Name:
Elena Pacifici
Education
2015
University of Rome “Sapienza”, Italy.
PhD studentship in Genetics and Molecular Biology.
2011
University of Rome “Sapienza”, Italy.
Master degree in Genetics and Molecular Biology.
Final mark: 110 /110 cum laude.
2009
University of Rome “Sapienza”, Italy.
Bachelor’s degree in Biological Sciences, curriculum in Genetics and
Molecular Biology.
Final mark: 100/110.
Research Experience
2015-present:
Research work as PostDoc in the laboratory of Prof.ssa Sabrina Sabatini
Dept. of Biol. and Biotec. “C. Darwin”-“Sapienza” University of Rome, Italy.
Research project: "Meccanismi molecolari coinvolti nella formazione di confini
cellulari nella radice di Arabidopsis".
Main focus of the work: "To understand which molecules are necessary to regulate
the correlation between cell size and cell fate in the Arabidopsis root".
2011 - 2014:
PhD Research training in the laboratory of Prof.ssa Sabrina Sabatini
Dept. of Biol. and Biotec. “C. Darwin”-“Sapienza” University of Rome, Italy.
Title of PhD project thesis: "Molecular mechanisms of interaction between cellular
expansion and cytokinin signaling".
Main focus of the work: To understand which molecules are necessary to
regulate the correlation between cell size and cell division and/or
differentiation input.
2009 - 2011:
Research training in the laboratory of Dott.ssa Sabrina Sabatini, Dept. of Biol.
and Biotec. “C. Darwin”-“Sapienza” University of Rome Italy.
Final dissertation: “Molecular mechanisms depending on the interaction
between cytokinin and auxin necessary to the maintenance of the Arabidopsis
thaliana root stem cell niche.
Pacifici E.
Main focus of the work: to investigate the molecular mechanisms controlling
the stem cell niche differentiation in the scr1-1 mutant after the ARR1 ectopic
expression in the root stem cell niche and to understand if ARR1 activates the
same molecular circuit acting at the transition zone .
2008 - 2009:
Undergraduate training in the Lab of Dott.ssa Sabrina Sabatini, Dept. of Biol.
and Biotec. “C. Darwin”-“Sapienza” University of Rome, Italy.
Final dissertation: Role of the cytokinin hormone in the maintenance of the
Arabidopsis thaliana root stem cell niche.
Main focus of the work: to determinate the relationship between SCR and
ARR1 genes and if the loss of function mutant of ARR1 gene complements the
scr1-1 mutation.
Fellowship and Awards
2011:
Fellowship for excellence in academic performance of PhD School in Genetics
and Molecular Biology - XXVII cycle - “Sapienza” University of Rome, Italy.
Research results including publications, teaching and meetings
Publications
Moubayidin L, Di Mambro R, Sozzani R, Pacifici E, Salvi E, Terpstra I, Bao D, van Dijken A,
DelloIoio R, Perilli S, Ljung K, Benfey PN, Heidstra R, Costantino P, Sabatini S. (2013) Spatial
coordination between stem cell activity and cell differentiation in the root meristem. Developmental
Cell. 26, 405-15.
Pacifici E, Polverari L, Sabatini S. (2015) Plant hormones crosstalk: the pivot of root growth. Journal
of Experimental Botany, doi:10.1093/jxb/eru534.
Teaching
2015 “Genome editing: the CRISPR/Cas9 technology” for the course of Molecular Biology of Model
System Development. Tutor: Prof.ssa Sabrina Sabatini, University of Rome “Sapienza”.
2015 “The plant cell wall” for the course of Molecular Biology of Model System Development.
Tutor: Prof.ssa Sabrina Sabatini, University of Rome “Sapienza”.
2013 “The plant cell wall” for the course of Molecular Biology of Plant Development. Tutor: Prof.ssa
Sabrina Sabatini, University of Rome “Sapienza”.
2012 “The plant cell wall of Arabidopsis thaliana model system” for the course of Molecular Biology
of Plant Development. Tutor: Prof.ssa Sabrina Sabatini, University of Rome “Sapienza”.
Pacifici E.
Meetings and Submitted Posters
2014- The International Symposium on Auxins and Cytokinins in Plant Development ACPD 2014Prague, Czech Republic.
PosterI: Elena Pacifici, R. Di Mambro, R. Sozzani, P. Benfey, W. Busch, P. Costantino, and S.
Sabatini. (2014) Molecular mechanisms of interaction between cellular expansion and cytokinin
signaling.
PosterII: Di Mambro R, De Ruvo M, Perilli S, Sozzani R, Pacifici E, Benfey P, Busch W, Costantino
P and Sabatini S. (2014) Tissue specific cross-talk between cytokinins and auxin is critical for
Arabidopsis root meristem growth and development.
2012- 2012 EMBO Conference Series Plant Development and Environmental interactions Matera,
Italy
PosterI: Elena Pacifici, P. Costantino, and S. Sabatini. (2012) Molecular mechanisms of interaction
between cellular expansion and cytokinin signaling.
Poster II: Laila Moubayidin, R. Di Mambro, E. Pacifici, I. Terpstra, S. Perilli, R. Dello Ioio, R.
Heidstra, P. Costantino, and S. Sabatini. (2012) SCARECROW sustains stem cell activity inhibiting
cytokinin dependent cell differentiation input.
Data processing knowledge
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Proficient in Microsoft Office, including Word, Power Point and Excel.
Proficient in ImageJ, Adobe Illustrator and Photoshop CS2, CS5 and CS6.
Proficient in online database Arabidopsis eFP Browser, The Arabidopsis Information
Resource (TAIR), Salk Institute Genomic Analysis Institute (SiGnAL), The European
Arabidopsis Stock Centre (uNASC).
Proficient in Confocal microscopy, trained with Zeiss LSM 780.
Proficient in Real Time PCR, trained with Applied Biosystems 7500 FAST Real Time
PCR.
Participation at workshop:
Course on “Real Time and Applications”- 28.07.2014 - Dept. Biology and Biotechnology, Via dei
Sardi, 70-Rome, Italy. Dr.ssa Serena Grimaldi-qPCR/CE Sr Associated Sales FAS Life Technologies
Brand.
Date 16.12.2015
Elena Pacifici