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Forensic and Scientific Services Testing for algal blooms and algal toxins Identification and enumeration, toxin gene detection, toxin analysis Why monitor algae? Routine monitoring of water bodies enables water utility operators and managers to ascertain risk, and develop frameworks to respond to the onset and progress of potentially toxic algal blooms in water. The ability to act quickly to changes in algal concentrations, through the instigation of a water treatment intervention to remove toxins and taste and odour products, is vital in water resource management. Our services Organic Chemistry offers a range of analytical tests for surveillance of algae in drinking, recreational and ornamental water bodies. Our expert team provides outstanding service delivery in: identification and enumeration of freshwater algae including cyanobacteria (blue-green algae) early detection of toxic blue green algae by DNA analysis algal toxin analysis (freshwater and marine). In addition our staff have expertise in the interpretation of analytical tests and can provide education and training and method research and development. Sampling requirements Analysis Volume minimum Container Preservation Storage Holding time Algae ID and counts 250mL 1L usual vol. HDPE plastic or glass Lugol’s iodine or as sampled Chilled (not frozen) Max 48hrs if not preserved Toxin genes 100mL HDPE plastic or glass N/A Chilled (not frozen) Max 48hrs Toxins LyngbyatoxinA/DSP ONLY 200mL scum material HDPE plastic or glass Sodium thiosulphate (if chlorinated) or as sampled Chilled 3-5 days >5 days needs to be frozen Algae/Algae toxin testing capability Analytical test Limit of reporting Method Approx. turnaround time *Cyanobacteria ID/counts (including potentially toxic algae) 25 cells/mL Phase contrast microscopy 2-3 working days *Biovolume (cyanobacteria) 0.01mm3/L Phase contrast microscopy 2-3 working days *Total Phytoplankton ID/counts 5 cells/mL Phase contrast microscopy 4 working days Blue green algae gene detection Presence/absence PCR/real time PCR 2 working days Microcystins 0.5µg/L HPLC/Diode Array UV detection 3-5 working days Saxitoxins (not saxitoxin equivalents) 2.0µg/L HPLC/Fluorescence detection 3-5 working days Cylindrospermopsin/ deoxycylindrospermopsin 0.2µg/L HPLC MS/MS 3-5 working days Lyngbyatoxin A/ marine toxins (DSPs) 0.5µg/L HPLC MS/MS 3-5 working days Nodularin 0.5µg/L HPLC MS/MS 3-5 working days 0.5µg/L HPLC MS/MS 3-5 working days Anatoxin-a *NATA accredited tests How can we help? For further information on the services available or to request a quote please contact Public and Environmental Health, Organic Chemistry: Algal identification and enumeration Karen Reardon Phone: (07) 3274 9090 Email: [email protected] Identification of toxin genes Barbara Sendall Phone: (07) 3000 9916 Email: [email protected] Toxin identification and quantitation Steve Carter Phone: (07) 3274 9085 Email: [email protected] 2 Testing for algal blooms and algal toxins