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Domain Archaea Prokaryotic Single celled Live in extreme environments Methanococcus janaschii Archaea examples • Halophiles in the Dead Sea. • Thermophiles in Yellowstone Domain Bacteria prokaryotic, single celled • Bacterial classified by shape cocci bacilli spirillum Streptococcus pyogenes Bacillus anthracis Treponema pallidum Bacteriology >10 trillion on/in a human Heterotrophs energy from breaking down organic compounds from environment Autotrophs make own food source example: cyanobacteria Cyanobacteria Oscillatoria Gleocapsa • Some bacteria are bioluminescent Examples of bacteria used in lab – non pathogenic E. coli K12 Bacillus subtilis , soil bacterium Staphlyococcus epidermis Found on skin, not pathogenic Gram stain – the first step in identifying disease-causing bacteria • The stain detects difference in the cell wall – Gram positive – contain peptidoglycan and stain purple – Gram negative - stain pink Results help decide which antibiotic to prescribe Examples: • Gram-positive cocci – Staphylcoccus aureus can cause skin infections and toxic shock syndrome; – Streptococcus pneumoniae can cause pneumonia. • Gram-negative cocci – Neisseria meningitidis causes meningitis – Neisseria gonorrhoeae causes the STD gonorrhea. • Gram-positive bacilli – Bacillus anthracis can cause skin infections or respiratory death – Listeria monocytogenes can cause foodborne illnesses. • Gram-negative bacilli – Escherichia coli is a common cause of urinary tract infections. Anatomy No nucleus May have flagella DNA Cell wall May have pili Binary fission is asexual reproduction • Some bacteria can be killed by antibiotics This antibiotic on a paper disc killed MRSA (vancomycin) This bacterial species is killed by antibiotic 1 and 3 but is resistant to 2 and 4