Download OM116L Manual

School Microscope
Manual
OM116L
Please Read this Manual thoroughly before use.
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Contents
Safety
2
Parts List
3
Features
3
Operation
4
Compound Microscope Parts
5-7
Maintenance
8
Contact Information
8
SAFETY
Do not disassemble any parts of the microscope, except where noted in the instruction manual.
In case of a problem with the microscope, contact a professional repairman or the manufacturing
factory.
The microscope should be kept at temperatures between 0C – 40C / 32F – 104F, with a maximum
humidity of 85%.
Do not place the instrument in direct sunlight or under direct indoor light. This environment can
influence the quality of the specimen imaging.
Do not place the instrument in a dusty environment. When not in use, cover the microscope with a dust
cover.
Keep the instrument on a level and sturdy surface.
Carry the microscope with two hands, one hand underneath the base unit and the other grasping the
“neck” of the microscope.
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Parts List
•
OM116 Compound Microscope
•
4X, 10X, 40X Objectives
•
One 10X Widefield Eyepiece
•
Three AA Batteries (Included)
•
One Instruction Manual
Features
In addition, the portable, lightweight OM116L can be moved around
the classroom (or house) with ease. A useful feature is a locknut to
secure the eyepiece - and to minimize damage from small hands!
The focus knob is a good size and also includes a slip clutch, also
to minimize the risk of damage.
In a nutshell, this microscope is a good solution for schools and
families when they wish to introduce the concepts of microscopy to
young students without the expense of a more advanced instrument.
The microscope stands 292mm high. The base measures
107mm x 145mm.
The Omano OM116L school microscope fulfills Omano's aim of balancing quality with
affordability. It is a compact, compound microscope that is ideal for customers who
require simplicity and affordability.
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Operation
Familiarization: First, familiarize yourself with all the parts of a microscope so that you
can easily move from one part to another during operation.
Moving and Placement: It is worth remembering that while a good quality microscope
will last a lifetime, it is a sensitive scientific instrument that will suffer damage from sharp
blows or impact. Always, therefore, carry your microscope in both hands. Grasp the arm
with one hand and place the other hand under the base for support. Always place the
microscope on a level and stable surface.
Slide Preparation: Microscope slides should always be prepared with a cover slip or
cover glass over the specimen. This will help protect the objective lenses if they touch
the slide. To hold the slide on the stage fasten it with the stage clips. You can push
down on the back end of the stage clip to open it.
Focusing the Microscope:
1. Start by turning the revolving nosepiece (turret) so that the lowest power
objective lens is "clicked" into position. The lowest power objective is the shortest
one. This objective is the easiest to focus and center the image in the field of
view.
2. Next turn on the light. Top light for, specimens that are visible to the naked eye,
low magnification, i.e. insects, crystals, rocks, leaves, etc. Bottom light for,
specimens not visible with the naked eye, higher magnifications that require
slides, i.e. cellular structures, bacteria, muscle tissue etc.
3. While looking at the objective lens and the stage from the side, turn the coarse
focus knob so that the stage moves upward toward the objectives. Move it as far
as it will go without touching the slide.
4. Now, look through the eyepiece(s) and adjust the illuminator and diaphragm until
you attain the maximum, comfortable level of light.
5. Slowly turn the adjustment so that the stage moves down (away from the slide).
Continue until the image comes into broad focus.
6. Move the microscope slide until the image is in the center of the field of view.
Then readjust the illuminator or diaphragm in order to attain the clearest image.
7. Once you have attained a clear image, you should be able to change to a higher
power objective lens with only minimal use of the focusing adjustment. If you
cannot focus on your specimen, repeat the above steps and work from objective
to objective until the higher power objective lens is in place.
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General Advice
1. Be careful not to allow the objective lens to touch the slide as you may break the
slide.
2. When using a monocular microscope, the correct technique is to look through the
eyepiece with one eye and keep the other eye open. Most new users, tend to
close one eye. While many microscopists do close one eye, you will help avoid
eye strain by keeping both eyes open.
3. Finally, remember! When you view a specimen through a microscope, you are
viewing an image through multiple lenses. As a result, the image is upside down
and back-to-front so when you move the slide to the right, the image goes to the
left and vice versa!
General Compound Microscope Parts and Information
A high power or compound microscope achieves higher levels of magnification than a
stereo or low power microscope. It is used to view smaller specimens such as cell
structures which cannot be seen at lower levels of magnification.
Essentially, a compound microscope consists of structural and optical components.
However, within these two basic systems, there are some essential components that
every microscopist should know and understand.
These key microscope parts are illustrated and explained below.
STRUCTURAL COMPONENTS
The three basic structural components of a compound microscope are the head, base
and arm.
•
•
Head/Body houses the optical parts in the upper part of the microscope
Base of the microscope supports the microscope and houses the illuminator
•
Arm connects to the base and supports the microscope head. It is also used to
carry the microscope.
When carrying a compound microscope always take care to lift it by both the arm and
base, simultaneously.
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(OM136C Shown above)
OPTICAL COMPONENTS
There are two optical systems in a compound microscope: Eyepiece Lenses and Objective
Lenses:
Eyepiece or Ocular is what you look through at the top of the microscope. Typically, standard
eyepieces have a magnifying power of 10x. Optional eyepieces of varying powers are available,
typically from 5x-30x.
Eyepiece tube holds the eyepieces in place above the objective lens. Binocular microscope heads
typically incorporate a diopter adjustment ring that allows for the possible inconsistencies of our
eyesight in one or both eyes. The monocular (single eye usage) microscope does not need a
diopter. Binocular microscopes also swivel (Interpupillary Adjustment) to allow for different
distances between the eyes of different individuals.
Objective Lenses are the primary optical lenses on a microscope. They range from 4x-100x and
typically, include, three, four or five on lens on most microscopes. Objectives can be forward or
rear-facing.
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Nosepiece houses the objectives. The objectives are exposed and are mounted on a rotating turret
so that different objectives can be conveniently selected. Standard objectives include 4x, 10x,
40x and 100x although different power objectives are available.
Coarse and Fine Focus knobs are used to focus the microscope. Increasingly, they are coaxial
knobs - that is to say they are built on the same axis with the fine focus knob on the outside.
Coaxial focus knobs are more convenient since the viewer does not have to grope for a different
knob.
Stage is where the specimen to be viewed is placed. A mechanical stage is used when working at
higher magnifications where delicate movements of the specimen slide are required.
Stage Clips are used when there is no mechanical stage. The viewer is required to move the slide
manually to view different sections of the specimen.
Aperture is the hole in the stage through which the base (transmitted) light reaches the
stage.
Illuminator is the light source for a microscope, typically located in the base of the
microscope. Most light microscopes use low voltage, halogen or LED bulbs with
continuous variable lighting control located within the base.
Condenser (if included) is used to collect and focus the light from the illuminator on to the
specimen. It is located under the stage often in conjunction with an iris diaphragm.
Iris Diaphragm (if included) controls the amount of light reaching the specimen. It is located
above the condenser and below the stage. Most high quality microscopes include an Abbe
condenser with an iris diaphragm. Combined, they control both the focus and quantity of light
applied to the specimen.
Condenser Focus Knob moves the condenser up or down to control the lighting focus on the
specimen.
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Maintenance
Care & Maintenance of Your Microscope
Your microscope will last a lifetime if cared for properly and we recommend that you
observe the following basic steps:
1. When finished viewing, lower the stage, click the low power lens into position and
remove the slide.
2. Switch off the microscope when not using.
3. Avoid touching the glass part of the lenses with your fingers. Use only special
lens paper to clean the lenses.
4. Dust is the number one enemy of a microscope, so always keep your microscope
covered when not in use. When not in use for extended periods, replace the
microscope in its box.
Contact Us:
Website: www.microscope.com
Phone: (877) 409-3556
Email: [email protected]
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