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Name __________________________________ Bi358 Final 200 points 1. Fill in the blanks below with one of the terms from the list provided. Each term may be used only once. There are more terms than blanks. 32 points (19 minutes) Aggregation Auxin Cysts Defense Developing a sulfide insensitive hemoglobin Dinitrogen Elemental sulfur Flagellae Horizontal gene transfer Hydrophobin Leghemoglobin Legume roots Meiosis Mitochondrial Motile Nitrate Nitrate reductase Nitrogenase Nod D Nod factor Nuclear Organellar gene mutation Oxidizing sulfide Prokaryotes Rhizobium Sessile Specificity Sulfate Symbiotic prokaryotes Transmission _______________________ is the predominant form of nitrogen on the planet. ______________________ is the enzyme that converts this form of nitrogen to a more biologically usable form. This enzyme is only present in ______________________. Flavenoids are compounds secreted by __________________ that diffuse into ___________________ and bind to the protein _____________________. This signaling step during symbiosis onset is one place for determining ____________________ between host and symbiont. The vast majority of proteins that function in the mitochondria are coded for by genes that reside in the _________________________ genome. This is an example of ______________________________that occurred through evolutionary time in the process of a symbiont becoming a true organelle. Zooxanthellae can be ______________________ in the freeliving state, as evidenced by the presence of _________________________. Zooxanthellae have never been observed to undergo ________________________. The clam Solemya offsets the toxic effects of sulfide by ___________________________ in the mitochondria. Subsequently the less toxic ________________________ is transported to the symbionts and used for chemosynthesis. ______________________ is an example of a ectomycorrhizal fungus protein that is upregulated during symbiosis onset. This protein is thought to function in hyphal __________________________ during sheath formation. 1 Name __________________________________ 2. Define the following terms. Answer only 5. 20 points (12 minutes). X-bacteria Leghemoglobin Monotropid plants Tetraselmis Mycetocyte Transposon mutagenesis Nod operon 2 Name __________________________________ 3. List the morphological and behavioral adaptations the giant clam Tradacna has made for symbiosis with zooxanthellae. 8 points (5 minutes.) ________________________________________________________________________ Question 4. 8 points (5 minutes) 4a. Describe (e.g. draw and label) the morphology of Frankia in culture away from its host plant. Describe its morphology in the symbiosis. 4b. List two examples of actinorhizal symbioses that are used in the management of forests and forest ecosystems and explain their usefulness. ________________________________________________________________________ Question 5. 8 points (5 minutes) Part A. According to the endosymbiosis theory, place in chronological order the following series of events that are thought to have given rise to the modern day diversity of organisms . Part B. List an extant (still living today) organism that is thought to have arisen from the organisms that resulted from these events. A. Endosymbiosis of a photosynthetic bacterium that eventually becomes a chloroplast B. Origin of life and formation of ancestral prokaryote C. Endosymbiosis of eubacterium that eventually forms mitochondria D. Ancestral prokaryote forms a nucleus Part A Order (A,B,C,or D) 1.___________________ 2.___________________ 3.___________________ 4.___________________ Question 6. 15 points (9 minutes). Part B Extant example ______________________________________ ______________________________________ ______________________________________ ______________________________________ 3 Name __________________________________ 6a. Draw and label a cross section through a typical crustose and fruticose lichen. Where does photosynthesis take place? Where do pockets of air get trapped? 6b. Draw and label a close-up of the physical interaction between one algal cell and the fungal host. ________________________________________________________________________ 7. Buchnera is thought to provide essential amino acids to its aphid hosts. Describe the aphid growth experiments that support this hypothesis. 15 points (9 minutes) 4 Name __________________________________ 8. Ectomycorrhizae and ericoid mycorrhizae are efficient at extracting nitrogen from organic soils. Briefly describe this process. 7 points (4 minutes) ________________________________________________________________________ 9. Juvenile vestimentiferan worms have been collected from vents. Describe their morphology and how it gives clues to the process of symbiosis onset in vestimentiferans and their chemoautotrophic symbionts. 7 points (4 minutes) ________________________________________________________________________ Question 10. 8 points (5 minutes) 10a. List two proposed functions of secondary metabolites in lichens. 10b. Secondary metabolites often appear as _____________________ on the outside of the algae. 10c. What happens to secondary metabolite production in the fungal partners when it is cultured separately from algae in the laboratory? 5 Name __________________________________ 11. How are the three major types of mycorrhizal associations distributed along a latitudinal and altitudinal gradient? Include in your answer, the major types of plants and fungi involved in each association and in what kinds of soils these associations predominately occur. 30 points (18 minutes) 6 Name __________________________________ 12. The onset of symbiosis between Rhizobium and legumes is a complex series of events that results in the formation of a symbiotic root nodule. Describe the events that occur after Rhizobium proliferates and migrates to the legume root and secretes nod factors. End your description with the formation of a young symbiotic nodule. 30 points (18 minutes) 7 Name __________________________________ 13. Briefly describe the technique you would use to measure the rate of nitrogen fixation of a pea plant grown in the laboratory. 12 points. (7 minutes) ________________________________________________________________________ 8