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Where does almost all energy come from for Earth? Do you have energy within your body? Potential Energy – stored energy (ATP molecules, Carbohydrate, Proteins and Lipids) Kinetic Energy – energy in motion (breaking down ATP to ADP, or any other molecule) JQ: Why do you think plants are green for most of the year, instead of red, orange or yellow? You need your textbook today. What’s in Sunlight? A prism, like the one shown below, is an object designed to alter light as it passes through it. *BE EXTREMELY CAREFUL WITH ALL THE EQUIPMENT* 1. Obtain a prism and a spectroscope, 2. Try to get the prism and spectroscope to separate white light into colors (might have to go near window) 3. Make a list of all the colors your prism produces, and take a picture if you can. Use your textbook to read about photosynthesis, the process that supplies all ecosystems with energy. Complete the note sheet as you go. What is photosynthesis? CO2 + H2O + Sunlight O2 + C6H12O6 The process of converting carbon dioxide, water, into oxygen and glucose. Sunlight supplies the energy for this process. The most important biological process on the planet : ) Who does photosynthesis? Plants, some bacteria, some protists, and even some (one known) animal can perform photosynthesis. Where does it occur? For plants, protists (and that one animal) it occurs inside the cells, there are special structures called chloroplasts. They are what make photosynthetic organisms green. Bacteria don’t have organelles, they perform it on their plasma membrane One chloroplast Where does it occur (con’t)? Each chloroplast is covered in 2 bilayers, and contain the following: 1. Granum (stacks of thylakoids) 2. Thylakoids (contain chlorophyll, light reactions occur on the membrane of these) 3. Stroma: liquid outside of the granum Silly way to remember how the chloroplast is organized: One chloroplast If the Chloroplast is the Company, Then the Grana (plural of granum) are the garages, And the Thylakoids are the Trucks How does it occur (overview)? The green pigment inside chloroplasts, called chlorophyll, absorb light energy from the sun, and the cell can use the energy to covert CO2 and H2O into sugar. Plants also have other pigments. How does CO2, O2, and Water get in and out of a plant? 1. Plants have pores on the bottom of their leaves called stomata They open and close to let Oxygen (O2) and Carbon dioxide (CO2) in and out. Water can leave through them as well. Water is taken into plants through roots. How does it occur (detailed)? Stage 1: Light reactions 1. What happens here: H2O + Light energy ATP + NADPH + O2 2. Light energy from the sun provides power for proteins to pump Hydrogen atoms (protons) from the outside of the thylakoid (called the stroma) to the inside of the thylakoid. Electrons are lost. Hydrogens (H+) MOVE AGAINST THEIR CONCENTRATION GRADIENT. WHAT KIND OF DIFFUSION IS THIS? Stroma H H H Thylakoid Membrane Inside thylakoid H H H H H H How does it occur (con’t)? Stage 1: Light reactions 1. Light also gives energy to proteins that convert NADP+ into NADPH, which is a high energy molecule that the cell can use is phase 2. Electrons are lost. Stroma Thylakoid Membrane Inside thylakoid NADPH H H H H H H H How does it occur (con’t)? Stage 1: Light reactions 1. The reason that plants need water is so that water can be split: electrons from water molecules are used to replenish those lost in process (Water is split into 2H+, O, and electrons) 2. There is a high concentration of protons (H+) accumulating inside the thylakoid. The H+ build up inside as they are pumped in during ETC, and from the H’s in H2O. 3. H+ moves down its concentration gradient through ATP synthase, a protein in the thylakoid membrane that makes ATP from ADP. This is called Chemiosmosis ATP, like NADPH, with be used in phase 2. Stroma Thylakoid Membrane Inside thylakoid H H H H H H H ATP Photosynthesis Recap for Phase 1 CO2 + H2O + Sunlight O2 + C6H12O6 We don’t know yet (Phase II) 1. H’s go into Thylakoid to establish Steep gradient 2. O combines with another O and diffuses out (this is why plants make oxygen) 3. Electrons from water replenish those lost phase I 1. Is used to Provide energy to proteins that Are pumping H’s Into thylakoid thru Active transport, And for proteins Making NADPH We don’t know yet (Phase II) 1. Comes from Splitting of water How does it occur (con’t)? Stage 2: Dark Reactions (Calvin Cycle) 1. What happens here: CO2 + ATP + NADPH C6H12O6 (Glucose) 2. CO2 moves through stomata (pores in bottom of leaves) from atmosphere, diffuses through cell membrane and through thylakoid membrane into the Stroma. (what type of diffusion occurs here?) 3. CO2 undergoes a series of chemical reactions with other molecules to form C6H12O6 (Glucose) . Energy from the ATP and NADPH are used to power the chemical reactions. NADP+ and ADP are sent back to light reactions 4. When CO2 is used in chemical reactions in order to form molecules inside our body, like glucose, it is called Carbon Fixation. Summary of Photosynthesis (con’t)? Both the light reactions and “dark” reactions are occurring at the same time within the chloroplast, but there are differences. Complete the chart to quiz yourself on some of the basics of photosynthesis Light Reactions Where does it occur? What are the reactants (chemicals that go into the process) What are the products (chemicals that are made from the process) Key players involved? Does this stage produce energy or use it? “Dark Reactions” / Calvin Cycle Can we capitalize on photosynthesis? For a very long time, nature has influenced mankind’s technology. So then, why are plants green? Absorption Spectrum for Photosynthetic pigments A. Which colors do chlorophyll absorb best? Plants are green because it helps them absorb blue and red light, which are the most energy-efficient colors to absorb from our sun. Alien Plants? Based on the colors of the plants on Pandora, how similar is the sunlight they receive, and their absorption spectrum is to the plants on earth? Wrap-Up Photosynthesis 1. What is it? 2. Who Does it? 3. Where does it happen? 4. Why is photosynthesis so important to life on earth? How do plants manage all this? After all, they are barely living, right? Journal Question What would happen if all of the plants on earth suddenly died today? Explain. JQ The man in the video has been exploring Antarctica alone for 86 days. He left himself food at checkpoints that he could find on his way back from the expedition. Watch the video. Why is he so excited for the goodies he has left? Would you react the same way? Try to imagine yourself in his position. Explain. Essential Question: If producers/autotrophs are responsible for supplying the rest of the world’s organisms with sugar molecules(glucose), then how do organisms use the sugar molecules? (glucose)? How do organisms get the energy from the food (Glucose)? Two ways. Aerobic Respiration – use oxygen to extract energy from glucose Fermentation– oxygen is NOT used to extract energy from glucose. Anaerobic process Look at the equation for Aerobic Cellular respiration. How does it compare to photosynthesis? C6H12O6 + 6O2 6H2O + 6CO2 + Energy (ATP) Photosynthesis & Cellular Respiration In photosynthetic organisms, the processes of photosynthesis and cellular respiration work together. The products of one can be used for the other Who does Respiration? Which of the following organisms need to extract energy from glucose to live? Plants? Bacteria? Fungi? Protists? Algae? Animals? YES! YES! YES! YES! YES! YES! Many organisms can do both aerobic and anaerobic, including humans Who are some of the main players (molecules) in the process? 1. Glucose 2. NAD+ and NADH (electron carriers for the electron transport chain in aerobic respiration) 3. FAD and FADH2 (electron carriers for the electron transport chain in aerobic respiration) a. b. c. d. What are the reactants in the equation for cellular respiration? oxygen and lactic acid carbon dioxide and water glucose and oxygen water and glucose Where does aerobic (cellular) occur? Inside the cells, there are special beanshaped structures called Mitochondria. Inside they are full of folds. How does it occur? Molecules of glucose are chemically “chopped up” in a series of chemical reactions, which produces CO2 and water molecules. Breaking the bonds in glucose is used to make ATP (Adenosine triphosphate), the cell’s energy molecule. ATP is a type of nucleotide. How does cellular respiration occur? Click 1. Glycolysis,, 2. Aerobic Respiration, 3. Fermentation to learn more 1. 2. Aerobic Respiration 3. Step 1: Glycolysis -Glycolysis is a chemical reaction that means “splitting glucose” -produces 2 ATP (net) during the process -occurs in the cytoplasm of all cells -all organisms can perform glycolysis -it is an anaerobic process, meaning no oxygen is required c c c c c c c c c + c c c During glycolysis, 6-C glucose is converted to 2, three-C pyruvate molecules. In the process: -2 ATP are used, but 4 ATP are made -2 NAD+ become 2NADH (used later) Summary: An overall gain of 2 ATP and 2 NADH are made during glycolysis Glycolysis provides a cell with a net gain of a. b. c. d. 2 ATP molecules. 4 ATP molecules. 18 ATP molecules. 36 ATP molecules. The starting molecule for glycolysis is a. b. c. d. ADP. pyruvic acid. citric acid. glucose. Step 2: Citric Acid (Kreb’s) Cycle -aerobic process: occurs with the use of oxygen (organisms that breathe oxygen prefer to do this process, and con only do anaerobic for a short time) -occurs in the mitochondrial matrix (inner mito) in several steps 1. One pyruvate, from glycolysis, is converted into CO2 and H2O. IN the process, 1 ATP, 3 NADH, and 1 FADH2 are made (per pyruvate) Where does the Carbon Dioxide and Water that we breathe out come from? -The Kreb’s (Citric Acid) cycle can occur 2 times per glucose molecule -for each glucose: The 10 NADH’s (from glycolysis and CAC) along with the 2 FADH2 used in next step Electron transport chain. Step 2 (Con’t): Citric Acid (Kreb’s) Cycle c c c Step 2 (con’t): Electron Transport Chain 1. the electron transport chain is a series of proteins that pass electrons to one another. They are embedded in the inner mitochondrial membrane 2. Electron carriers NADH and FADH2 donate high energy electrons and hydrogen ions (H+) to ETC 3. Energy from the electrons are lost as they are passed through ETC, and energy is used to pump H+ against its concentration gradient using Active Transport 4. As H+ passively diffuses from inter membrane space to matrix, ATP synthase is powered to make ATP. 5. The electrons and H+ combined with oxygen in the matrix to produce water. for each glucose: 22 ATPs are made from the NADH and FADH2 in ETC Where does the oxygen come from? What would happen if you did not have oxygen at the end of the ETC? Step 2 (con’t): ETC Step 2, if no O2 is present: Fermentation -Anaerobic process: occurs without the use of oxygen -begins with glycolysis,producing 2 ATP (net) during the process -occurs in the cytoplasm of all cells -pyruvate is converted to either lactic acid or alcohol (ethanol), depending on the organism that is doing it -Without oxygen, glycolysis would continue to make NADH from NAD+. Once all the NAD+ is used up, glycolysis would stop, ATP would not be made, and organism would die. THE POINT OF LACTIC ACID and ALCOHOLIC FERMENTATION IS TO REGENERATE NAD+ for GLYCOLYSIS Lactic Acid Fermentation: Turns pyruvic acid into lactic Acid – regenerating NAD+ from NADH (so glycolysis can continue) Alcoholic Fermentation: Turns pyruvic acid into ethanol - regenerating NAD+ from NADH (so glycolysis can continue) When there is no/not enough oxygen present, your cells will begin to make lactic acid instead of pyruvate: A. Explain why oxygen is so important for aerobic cellular respiration? B. Explain why Kreb’s cycle is called an “aerobic” process even though it involves no oxygen directly in the cycle C. Explain what exactly happens when there is no oxygen to accept the electrons at the end of the ETC. DID YOU KNOW? The vitamins, like B6, and B12, found in green shakes that we talked about are needed to make the Kreb’s cycle run efficiently. A. Energy B. Light RXN C. Dark RXN X. Oxygen Y. glucose Splitting H2O Summary of Cellular Respiration Complete the chart to quiz yourself on some of the basics of cellular respiration Glycolysis Where does it occur? What are the reactants (chemicals that go into the process) What are the products (chemicals that are made from the process) Does it require oxygen? What type of organisms use this process, and when would they use it? cytoplasm Glucose, NAD+ ATP 2 ATP, 2 NADH, 2 Pyruvate NO ALL ORGANISMS Aerobic Respiration Mitochondria Pyruvate, NAD+, ADP, FAD Fermentation Cytoplasm Pyruvate, NADH Pyruvate in Krebs: ETC: 2 NAD+, and either 1 ATP, 3CO2, ATP, H2O lactic acid or 4 NADH, 1 FADH2 ethanol (alcohol) YES Most: plants, animals, bacteria, fungi, and protists if O2 is present NO Certain bacteria and fungi (yeast), as well as animals for brief time w/o O2 Molecule # of these molecules made from glycolysis # of these molecules made from Kreb’s Cycle # of ATP these molecules are equivalent to 1. ATP 2. NADH 2 2 2 (1 per pyruvate) 8 (4 per pyruvate) 4 30 3. FADH2 0 2 (1 per pyruvate) 4 Journal Question: Respond to the following 1. Most ATP in eukaryotic cells is produced in this organelle 2. Most ATP produced in aerobic respiration occurs in this process 3. Products of glycolysis include: 4. Products of the Krebs cycle include: 5. This is the final electron acceptor at the end of the ETC. 6. In the presence of oxygen, all cells synthesize ATP via the process of glycolysis. Many cells also can metabolize pyruvate if oxygen is not present, via the process of: 7. The net result of the breakdown of glucose in glycolysis and fermentation is the production of this many ATPs 8.This stage of cellular respiration requires ATP to proceed 9. Which stage of aerobic respiration produces ATP and NADH and releases CO2? 10. As H+’s flow through this protein ______ , energy is released and used to combine ADP and a phosphate to form ATP. Khan Academy: Link on Picture