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2-in-1 Portable LED Microscope Manual OM 115-LD Please Read this Manual thoroughly before use. www.microscope.com Page 1 Contents Safety 3 Parts List 4 Features 5 Operation 6-7 Compound Microscope Parts 8-11 Maintenance 12 Contact Information 13 www.microscope.com Page 2 SAFETY Do not disassemble any parts of the microscope, except where noted in the instruction manual. In case of a problem with the microscope, contact a professional repairman or the manufacturing factory. The microscope should be kept at temperatures between 0C – 40C / 32F – 104F, with a maximum humidity of 85%. Do not place the instrument in direct sunlight or under direct indoor light. This environment can influence the quality of the specimen imaging. Do not place the instrument in a dusty environment. When not in use, cover the microscope with a dust cover. Keep the instrument on a level and sturdy surface. Carry the microscope with two hands, one hand underneath the base unit and the other grasping the “neck” of the microscope. www.microscope.com Page 3 Parts List OM115 Duo Scope Microscope One Eyepiece: Wide field 10x Five blank slides 5 prepared slides Slide labels Five cover slips Fifty sheets of lens paper One plastic test tube One plastic Petri dish Forceps One straight dissecting needle One Eosin Y stain One Methylene Blue stain One Dropper One swab One wooden rod One vinyl dust cover One instruction manual www.microscope.com Page 4 Omano OM115LD Features The Omano OM115L Duo scope offers an innovative solution to the age old question: Stereo or Compound for that first microscope? The OM115L is both! By the simple addition of a top light, this microscope can be used to view cellular structures on the included slides or objects visible to the naked eye: insects, crystals, leaves etc...... AND, over 15 useful accessories are included. The Duo scope is of durable metal construction, designed to handle years of daily use by younger biologists. The viewing head is rotatable 360 degrees and allows students or siblings to easily view the same specimen without having to move the scope. The included 10x eyepiece and 4x, 10x, and 40x objectives enable magnifications of 40x, 100x, and 400x and like all other products we offer, are made with real precision, ground, and optical glass lenses. Good optics and dual focusing knobs make for easy focusing and clear viewing, even for novices. Upper and lower LED lighting make the OM115L Duo scope two microscopes in one (hence, the name Duo-Scope...). It functions as both a compound microscope and a dissecting microscope, meaning both cellular structures on the included slides or objects that are viewable to the naked eye can be observed. Cordless battery powered LED illumination operates on 3 AA batteries and makes this microscope completely portable for field study. Another attractive feature of LED illumination is that it is very efficient on power usage and also remains safely cool to the touch, unlike halogen or other light sources. In our opinion, the adaptability, portability, and overall quality of the OM115L Duo scope is a combination of qualities yet unseen at such a low cost and is sure to not disappoint! www.microscope.com Page 5 Operation Familiarization: First, familiarize yourself with all the parts of a microscope so that you can easily move from one part to another during operation. Moving and Placement: It is worth remembering that a microscope is a sensitive scientific instrument that will suffer damage from sharp blows or impact. Always, therefore, carry your microscope in both hands. Grasp the arm with one hand and place the other hand under the base for support. Always place the microscope on a level and stable surface. Slide Preparation: Microscope slides should always be prepared with a cover slip or cover glass over the specimen. This will help protect the objective lenses if they touch the slide. To hold the slide on the stage fasten it with the stage clips. You can push down on the back end of the stage clip to open it. Focusing the Microscope: 1. Start by turning the revolving nosepiece (turret) so that the lowest power objective lens is "clicked" into position. The lowest power objective is the shortest one. This objective is the easiest to focus and center the image in the field of view. 2. Next turn on the light. Top light for, specimens that are visible to the naked eye, low magnification, i.e. insects, crystals, rocks, leaves, etc. Bottom light for, specimens not visible with the naked eye, higher magnifications that require slides, i.e. cellular structures, bacteria, muscle tissue etc. 3. While looking at the objective lens and the stage from the side, turn the coarse focus knob so that the stage moves upward toward the objectives. Move it as far as it will go without touching the slide. 4. Now, look through the eyepiece(s) and adjust the illuminator and diaphragm until you attain the maximum, comfortable level of light. 5. Slowly turn the adjustment so that the stage moves down (away from the slide). Continue until the image comes into broad focus. 6. Move the microscope slide until the image is in the center of the field of view. Then readjust the illuminator or diaphragm in order to attain the clearest image. 7. Once you have attained a clear image, you should be able to change to a higher power objective lens with only minimal use of the focusing adjustment. If you cannot focus on your specimen, repeat the above steps and work from objective to objective until the higher power objective lens is in place. www.microscope.com Page 6 General Advice 1. Be careful not to allow the objective lens to touch the slide as you may break the slide. 2. When using a monocular microscope, the correct technique is to look through the eyepiece with one eye and keep the other eye open. Most new users, tend to close one eye. While many microscopists do close one eye, you will help avoid eye strain by keeping both eyes open. 3. Finally, remember! When you view a specimen through a microscope, you are viewing an image through multiple lenses. As a result, the image is upside down and back-to-front so when you move the slide to the right, the image goes to the left and vice versa! General Compound Microscope Parts and Information A high power or compound microscope achieves higher levels of magnification than a stereo or low power microscope. It is used to view smaller specimens such as cell structures which cannot be seen at lower levels of magnification. Essentially, a compound microscope consists of structural and optical components. However, within these two basic systems, there are some essential components that every microscopist should know and understand. These key microscope parts are illustrated and explained below. STRUCTURAL COMPONENTS The three basic structural components of a compound microscope are the head, base and arm. Head/Body houses the optical parts in the upper part of the microscope Base of the microscope supports the microscope and houses the illuminator Arm connects to the base and supports the microscope head. It is also used to carry the microscope. When carrying a compound microscope always take care to lift it by both the arm and base, simultaneously. www.microscope.com Page 7 (OM136C Shown above) www.microscope.com Page 8 OPTICAL COMPONENTS There are two optical systems in a compound microscope: Eyepiece Lenses and Objective Lenses: Eyepiece or Ocular is what you look through at the top of the microscope. Typically, standard eyepieces have a magnifying power of 10x. Optional eyepieces of varying powers are available, typically from 5x-30x. Eyepiece tube holds the eyepieces in place above the objective lens. Binocular microscope heads typically incorporate a diopter adjustment ring that allows for the possible inconsistencies of our eyesight in one or both eyes. The monocular (single eye usage) microscope does not need a diopter. Binocular microscopes also swivel (Interpupillary Adjustment) to allow for different distances between the eyes of different individuals. Objective Lenses are the primary optical lenses on a microscope. They range from 4x-100x and typically, include, three, four or five on lens on most microscopes. Objectives can be forward or rear-facing. Nosepiece houses the objectives. The objectives are exposed and are mounted on a rotating turret so that different objectives can be conveniently selected. Standard objectives include 4x, 10x, 40x and 100x although different power objectives are available. Coarse and Fine Focus knobs are used to focus the microscope. Increasingly, they are coaxial knobs - that is to say they are built on the same axis with the fine focus knob on the outside. Coaxial focus knobs are more convenient since the viewer does not have to grope for a different knob. Stage is where the specimen to be viewed is placed. A mechanical stage is used when working at higher magnifications where delicate movements of the specimen slide are required. Stage Clips are used when there is no mechanical stage. The viewer is required to move the slide manually to view different sections of the specimen. www.microscope.com Page 9 Aperture is the hole in the stage through which the base (transmitted) light reaches the stage. Illuminator is the light source for a microscope, typically located in the base of the microscope. Most light microscopes use low voltage, halogen or LED bulbs with continuous variable lighting control located within the base. Condenser (if included) is used to collect and focus the light from the illuminator on to the specimen. It is located under the stage often in conjunction with an iris diaphragm. Iris Diaphragm (if included) controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include an Abbe condenser with an iris diaphragm. Combined, they control both the focus and quantity of light applied to the specimen. Condenser Focus Knob moves the condenser up or down to control the lighting focus on the specimen. Maintenance Care & Maintenance of Your Microscope Your microscope will last a lifetime if cared for properly and we recommend that you observe the following basic steps: 1. When finished viewing, lower the stage, click the low power lens into position and remove the slide. 2. Switch off the microscope when not using. 3. Avoid touching the glass part of the lenses with your fingers. Use only special lens paper to clean the lenses. 4. Dust is the number one enemy of a microscope, so always keep your microscope covered when not in use. When not in use for extended periods, replace the microscope in its box. www.microscope.com Page 10 Contact Us: Website: www.microscope.com Phone: (877) 409-3556 Email: [email protected] www.microscope.com Page 11