Download catalogue #mlt 210 - Moberly Area Community College

CATALOG# MLT 210
CIP #51.1004
DATE: January 2017
Moberly Area Community College
Common Syllabus
MLT 210: Immunology
Current Term
Instructor:
Office number:
Office hours:
Contact information:
Classroom number:
Class days and time:
Catalog description: MLT 210 Immunology (w/clinicals)
1–4–2
The course covers concepts and applications of immunology, the immune system, and how
to perform and interpret associated tests. The student must satisfactorily perform in a
laboratory setting as well as pass written tests.
Prerequisite:
HSC171, BIO205, BIO209 and MLT150
Text:
Rittenhouse-Olson and De Nardin. (2013). Contemporary Clinical Immunology and
Serology. Upper Saddle River, New Jersey: Pearson. 0-13-510424-6
Other Required Materials:
Handouts, videos, training aids and transparencies as provided by the instructor. Library
assignments, periodicals, computerized modules, and guest speakers may be used as
appropriate.
Purpose of the course:
In this course, students study the basic concepts of immunology, the theory of clinical
assays performed in serology and molecular testing, and disease states involving the
immune system. Course content includes basic elements of immune response but also case
studies and interpretation of test results.
Cognitive Course Objectives:
Upon successful completion of this course, students will be able to:
1. Differentiate the components of the immune system and describe the process of
recognition and disposal of foreign material.
4/30/2017
1
2. Describe the cells of the immune system and discuss their interactions.
3. Describe and compare the functions of mediators of the immune system, for
example complement, cytokines, and interleukins.
4. Describe major methodologies used to diagnose immunological disorders including
the following:
Agglutination
Precipitation
Nephelometry
Turbidimetry
Electrophoretic techniques
Immunoassays (EIA, ELISA, IFA, DFA, RIA)
Molecular Methods
5. Discuss the types of specimens used for serological testing, when they are collected,
how they are handled, specimen quality and sources of error involving specimens.
6. Compare primary and secondary immune responses.
7. Discuss the etiological agents, signs and symptoms, complications of infection, and
diagnostic methods for streptococcal infections.
8. State the etiological agent, discuss the signs, symptoms, and progression of
infection, and describe testing methods used in the diagnosis of syphilis.
9. Describe the etiological agent, discuss the nature of the infections, and describe the
methods used to diagnose the following:
Tick-borne diseases
Infectious mononucleosis
Gp. A strep
Hepatitis
10. Discuss the purpose of testing for cold agglutinins and describe the procedure.
11. Describe the etiological agent, discuss the nature of the infection, and explain the
methods used to diagnose infection with Toxoplasma gondii.
12. Discuss autoimmune disease states and diagnostic testing used in the clinical
laboratory.
Psychomotor Course Objectives:
Students are responsible for knowing how to do the following to minimal competency
standards. In order to develop skills to this level, they will receive instruction and practice
in the student lab and at the affiliate site.
1. Identify correct specimen collection for specified immunological determinations
and correctly process specimens for testing.
2. Follow laboratory policies for record keeping and reporting.
3. Follow written procedures.
4. Evaluate specimen quality and describe corrective action to solve problems with it.
5. Safely handle and dispose of infectious materials.
6. Utilize quality controls in immunological procedures, evaluate performance from
QC results, and initiate corrective action if quality control fails.
7. Perform daily maintenance routines as directed by mentors.
8. Correctly perform specified immunological testing such as agglutination,
precipitation, EIA, etc. and interpret results.
9. Discuss the test principles, sources of error, expected values, biological false
positives or negatives, and clinical significance of immunological procedures not
performed in the laboratory such as FTA-AB, C3, C4, Toxoplasma antibody, and
Lyme Disease antibody.
10. Troubleshoot procedures and take appropriate action as needed.
4/30/2017
2
Affective Course Objectives:
At the end of the course the student will be able to display for following behaviors and
attitudes:
1.
2.
3.
4.
5.
6.
Demonstrate responsibility toward patients and colleagues.
Maintain confidentiality.
Accept instruction and constructive criticism.
Demonstrate initiative and resourcefulness.
Exhibit the ability to work independently.
Demonstrate attention to detail and quality
Course Content:
I.
Definition of immunity
A. First line of defense
B. Natural immunity
1. Cellular
a. Neutrophils
b. Eosinophils
c. Basophils
d. Mast cells
e. Monocytes
f. Tissue macrophages
g. Dendritic cells
h. Phagocytosis
2. Humoral
a. C-reactive protein
b. Serum amyloid A
c. Complement
d. Mannose-binding protein
e. Alpha-1 antitrypsin
f. Haptoglobin
g. Fibrinogen
h. Ceruloplasmin
C. Adaptive immunity
1. Cellular
2. Humoral
II.
Antibody characteristics
A. Immunoglobin classes
B. Antibody structure
3. Fab, Fc, and hinge molecular components
4. IgM
5. Immunoglobulin variants
C. Antibody synthesis
1. Primary response
2. Secondary response
4/30/2017
3
D. Functions
E. Immune complexes
F. Monoclonal antibody
III.
Antigen characteristics
D. General characteristics
E. Histocompatibility antigens
F. Autoantigens
G. Blood group antigens
H. Chemical nature of antigens
I. Physical nature of antigens
IV.
Cellular Immunity
A. T-cell development
B. Cytokines
a. Interferons
b. Tumor necrosis factor
c. Interleukins
d. Chemokines
e. Interferons
C. Assays for measurement
V.
Complement
A. Classical pathway
B. Alternative pathway
C. Lectin pathway
D. System controls
1. Fluid phase regulators
2. Cell-bound regulators
E. Receptors that amplify the immune response
F. Disease states
G. Laboratory assays and findings
VII.
Laboratory methods
A. Precipitation
B. Radial Immunodiffusion
C. Immunoelectrophoresis
D. Immunofixation electrophoresis
E. Agglutination
1. Direct agglutination
2. Passive agglutination
3. Reverse passive agglutination
4. Agglutination inhibition
F. Labeled immunoassays
1. RIA
2. EIA/ELISA
3. IFA
4. FPIA
G. RAST
H. Molecular Methods
4/30/2017
4
1.
2.
3.
VIII.
IX.
X.
XI.
Nucleic acid structure and organization
Nucleotide structure and sequence
DNA and RNA molecular structure and function
a. Genes and genetic code
b. Chromosomes
c. Non-chromosomal elements of a genome
d. Replication
4.
Nucleic acid-based methods
a. Nucleic acid hybridization methods
b. Solid support
(1) Filter
(2) Southern
(3) Sandwich
5.
Amplification
a. PCR
Autoimmunity
A. Hypersensitivity
B. Diseases of autoimmunity
1. Lupus erythematosis
a. Clinical signs
b. Immunologic findings
c. Laboratory diagnosis
2. Rheumatoid arthritis
a. Clinical signs
b. Immunologic findings
c. Laboratory diagnosis
C. Organ and Tissue specific Autoimmunity
1. Hashimoto’s thyroididis
2. Grave’s disease
3. Type I diabetes mellitus
4. Multiple Sclerosis
5. Myasthenia gravis
6. Goodpasture’s syndrome
Immunoproliferative Diseases
A. Multiple myeloma
B. Leukemias
C. Lymphomas
D. Waldenstrom Mcroglobulinemia
Transplant Immunology
A. Histocompatibility
B. Mechanisms of rejection
C. Graft-Versus-Host Disease
Acquired Immunodeficiency
A. Malnutrition
B. HIV/AIDS
C. Hepatitis
4/30/2017
5
XII.
XIII.
D. Herpesviridae
1. Epstein-barr
2. Cytomegalovirus
3. Herpes Simples 1 & 2
4. Varicella Zoster
5. Rubella
6. Measles
7. Mumps
Bacterial Serology
A. Host defense mechanisms
B. Streptococci
C. Helicobacter Pylori
D. Mycoplasma Pneumoniae
E. Rickettsia
F. Clostridium difficile
G. Spirochetes
Laboratory Safety
A. Personal Protective Equipment
B. Universal Precautions
C. Post-exposure treatment
Connection with Career and Technical Education (CTE) Outcome Statement:
In compliance with MACC’s CTE outcomes, the student who successfully completes this
course will be able to:
Outcome I: Demonstrate effective written and oral communication.
Organize for priority and efficiency.
Strictly follow written procedures and verbal instructions
Communicate in a professional way with instructors and other students
Outcome II: Demonstrate an understanding of discipline-related math and scientific
principles
Discuss the test principles, sources of error, expected values, biological false
positives or negatives, and clinical significance of immunological
procedures not performed in the laboratory such as FTA-AB, C3, C4,
Toxoplasma antibody, and Lyme Disease antibody
Outcome III: Think critically while systematically assessing problems, identifying issues
and implementing solutions.
Discuss the types of specimens used for serological testing, when they are
collected, how they are handled, specimen quality and sources of error
involving specimens
Outcome IV: Apply principles of professionalism and safety standards when entering the
workforce.
Demonstrate proper hand washing technique.
4/30/2017
6
Safely handle and dispose of infectious materials.
Follow laboratory policies for record keeping and reporting.
Outcome V: Demonstrate skill mastery and will pursue lifelong learning in their filed
while adapting to changing industry standards and following evidence-based practices.
Strictly follow written procedures and verbal instructions
Demonstrate initiative and resourcefulness; learn more than the minimum
Exhibit a professional appearance and attitude
ASSESSMENT OF STUDENT LEARNING:
In both the didactic and the laboratory portions of the course, the student must achieve
78% or greater. Failure to achieve this minimum score will result in dismissal from the
program. In the laboratory portion of the course, the final grade will be recorded as
“Pass” or “Fail” and registered with the didactic portion.
The following grading scale applies to all programs within the Allied Health Division:
100 – 92% = A
83 – 91% = B
78 – 82% = C
66 – 77% = D
65% and below = F
In the lecture portion of the clinical course, the final grade is derived from student
performance on examination(s) and/or assignments.
Grading/student assessment of lecture (didactic) portion of the course:
Final grade will be composed of:
Unit tests averaged 60%
Quizzes, case studies, study 10%
questions, or other assignments,
attendance and punctuality
averaged
Final Exam 30%
Total: 100%
Grading/student assessment of laboratory (student laboratory) portion of the course:
Final PASS/FAIL grade will be composed of:
Laboratory Exercises averaged 30%
Professional Behaviors Evaluation 70%
Total: 100%
Program Outcomes and Assessments:
The Allied Health Department continually strives to meet the needs of the Medical
Laboratory Technician student through program improvements. This is a cooperative
4/30/2017
7
effort that includes input from the faculty, student, Medical Laboratory Technician
Advisory Board, and other appropriate agencies or entities. Students are assessed on
mastery of the course concepts and essential skills throughout the courses of the Medical
Laboratory Technician Program. Other program assessments include clinical performance
criteria, essential skills mastery, the clinical process evaluation, ASCP examination scores,
placement rates, and follow-up surveys.
INSTRUCTOR POLICIES:
Tardiness and Attendance:
Students are expected to prepare for and attend all classes and clinical practice. Regular
attendance improves probability for success in the program. Habitual tardiness and
frequent absences are disruptive to the classroom and cause an unsafe environment in the
student laboratory. Being on time is mandatory.
Instructors carefully plan learning experiences, so it is important as a matter of courtesy
and fairness to the class that all individuals be present. Students absent for reasons
beyond their control, such as verified personal illness or family illness and/or death, can
make up class work. If a student misses so many classes due to extenuating
circumstances that the instructor feels the student cannot catch up, the MLT Program
Coordinator will send a written report to the Director of Allied Health.
Make-up and late work:
See the MLT Student Handbook for guidelines. It is up to instructor’s discretion to
reduce grade by certain percentage should the need arise if student turns in work or
assignments late. Remember that communication, accountability and responsibility are
very important professional behaviors.
Extra-credit work:
Extra-credit work is up to instructor’s discretion and is not a mandatory.
Other:
Instructors of this program expect the following from students:
1. Come to class prepared to discuss or apply important concepts by having read the
assigned material or reviewed materials for instrument operation.
2. Participate in class by listening, taking notes, and making contributions to
discussions.
3. Consult with faculty for clarification of difficult material or additional resources
to consult.
4. Respect the learning environment by averting distractions and disturbances such
as ringing cell phones and extraneous conversation in class.
5. Treat instructors and fellow students with consideration, concern, and fairness.
6. Cell phones must be turned off during lectures and laboratories.
7. Students may use a computer ONLY with the instructor’s approval.
8. No cell phones, programmable calculators, MP players, or IPODS may be in the
classroom during tests. Students may not wear hats during tests.
9. Please refer to the MLT handbook for the list of all policies of the department.
4/30/2017
8
COLLEGE POLICIES
Attendance:
Any student who misses two consecutive weeks of class during a regular sixteen-week
semester or the equivalent proportion of class time during a shorter session will be
dropped from the class by the instructor unless acceptable justification is supplied.
Additionally, any student who misses more than one-fourth of the entire number of inseat class meetings in a regular 16-week semester or the equivalent proportion of class
time during a shorter session, may be dropped from that class by the instructor if, in the
opinion of the instructor, the student does not have reasonable opportunity to succeed in
the class. A student’s attendance rate will be calculated based upon the first day of
the semester (not the student’s date of enrollment in the course).
Student attendance must be defined in a different manner for online, hybrid, and virtual
courses. Student attendance in these courses is defined as active participation in the
course. Online, hybrid, and virtual courses will, at a minimum, have weekly mechanisms
for student participation, such as any or all of the following methods:
a. Completion of quizzes or exams
b. Submission of assignments
c. Participation in threaded discussions
d. Communication with the instructor
A student who does not participate in an online, hybrid, or virtual course for two
consecutive weeks will be dropped by the instructor unless acceptable justification is
supplied. As with ground courses, a student’s attendance rate in online courses will
also be calculated based upon the first day of the semester. If a student does not
demonstrate active participation in the online course within the first two weeks (or
the equivalent proportion of class time during a short session), the student will be
dropped as “never attended.” Simply logging into an online class does not constitute
active participation.
Students should be aware that their dropping a course and their last date of
attendance in the course may impact their financial aid.
Academic Dishonesty:
MACC board policy is as follows: “Academic dishonesty by students damages
institutional credibility and unfairly jeopardizes honest students; therefore, it will not be
tolerated in any form.” Forms of academic dishonesty include but are not limited to the
following: violations of copyright law, plagiarism, fabrication, cheating, collusion, and
other academic misconduct. Incidents of dishonesty regarding assignments,
examinations, classroom/laboratory activities, and/or the submission of misleading or
false information to the College will be treated seriously. The procedure for handling
academic dishonesty is outlined in the Student Handbook (Policy Handbook M.010). In
cases of alleged academic dishonesty, the burden of proof is on the student, not on the
instructor.
4/30/2017
9
ADA Statement:
Students who have disabilities that qualify under the Americans with Disabilities Act
may register for assistance through the Office of Access and ADA Services. Students are
invited to contact the Access Office to confidentially discuss disability information,
academic accommodations, appropriate documentation and procedures. For more
information, please call either the Moberly office at (660) 263-4100 x 11240 or the
Columbia office at (573) 234-1067 x 12120, or visit our web page at
http://www.macc.edu/index.php/services/access-office.
Title IX Statement:
MACC maintains a strict policy prohibiting sexual misconduct in any form,
including sexual harassment, sexual discrimination, and sexual violence. All
MACC employees, including faculty members, are considered mandated reporters
of sexual misconduct and as such are expected to contact the Title IX Coordinator
when they become aware, in conversation or in writing, of an incident of sexual
misconduct. For more information on this policy or to learn about support
resources, please see http://www.macc.edu/sexual-misconduct-policy or contact
Dr. Jackie Fischer, MACC’s Title IX Coordinator, at 660-263-4110, ext. 11236 or
[email protected].
4/30/2017
10