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Dendritic cells are functionally defective in multiple myeloma: the role of interleukin-6 by Marina Ratta, Francesco Fagnoni, Antonio Curti, Rosanna Vescovini, Paolo Sansoni, Barbara Oliviero, Miriam Fogli, Elisa Ferri, Gioacchino Robustelli Della Cuna, Sante Tura, Michele Baccarani, and Roberto M. Lemoli Blood Volume 100(1):230-237 July 1, 2002 ©2002 by American Society of Hematology Alloreactivity of circulating DCs (PBDCs) and DCs derived from CD14+ monocytes (Mo-DC) from the same MM patients and of PBDCs from healthy subjects.Increasing numbers of DCs were tested for their capacity to stimulate 5 × 104 allogeneic CD3+ cells. Marina Ratta et al. Blood 2002;100:230-237 ©2002 by American Society of Hematology Capability of PBDCs and Mo-DCs from the same MM patients and of PBDCs from healthy subjects to present soluble antigens to autologous T cells.CD3+ cells (1 × 105) were incubated with either a fixed number (3000) of DCs (A) or increasing numbers of DCs (B). Marina Ratta et al. Blood 2002;100:230-237 ©2002 by American Society of Hematology Differentiation of DCs derived from CD34+ cells is inhibited by IL-6.CD34+ progenitor cells were incubated with GM-CSF, TNF-α, SCF, and FLT3-L (G+T+S+F) with or without IL-6. Marina Ratta et al. Blood 2002;100:230-237 ©2002 by American Society of Hematology Addition of IL-6 resulted in maintenance of DC high phagocytic capacity.CD34+ cells were cultured for 12 to 14 days with GM-CSF, TNF-α, SCF, and FLT3-L (G+T+S+F) with or without IL-6. Marina Ratta et al. Blood 2002;100:230-237 ©2002 by American Society of Hematology Alloreactivity of myeloma DCs derived from CD34+ cells generated in the presence of GM-CSF, TNF-α, SCF, and FLT3-L (G+T+S+F) and grown in FCS (A) or autologous serum (B) with and without IL-6.Increasing numbers of DCs were tested for their capacity to stimu... Marina Ratta et al. Blood 2002;100:230-237 ©2002 by American Society of Hematology Effect of IL-6 on the antigen-presenting ability (to autologous cells) of myeloma DCs derived from CD34+progenitors in the presence of GM-CSF, TNF-α, SCF, and FLT3-L (G+T+S+F) and grown in FCS (A) or autologous serum (B).CD3+ cells (1 × 105) were incubated ... Marina Ratta et al. Blood 2002;100:230-237 ©2002 by American Society of Hematology
